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中国农学通报

中国农学通报 ›› 2016, Vol. 32 ›› Issue (11): 39-42.doi: 10.11924/j.issn.1000-6850.casb15110107

所属专题： 食用菌； 食用菌

• 食品 营养 检测 安全 • 上一篇    下一篇

香菇多糖提取工艺优化及其抗氧化与抑菌功效研究

张敏瑜,齐延林,杨弘华,严章雪,汪波   

  1. 北京师范大学珠海分校,北京师范大学珠海分校,北京师范大学珠海分校,北京师范大学珠海分校,北京师范大学珠海分校
  • 收稿日期:2015-11-19 修回日期:2016-01-12 接受日期:2016-01-22 出版日期:2016-04-20 发布日期:2016-04-20
  • 通讯作者: 汪波
  • 基金资助:
    广东省大学生创新训练项目“食用菇多糖超声提取及抑菌效果研究”(201413177033)。

Optimized Extraction Condition of Lentinan and Its Antibacterial and Antioxidant Activity

张敏瑜,齐延林,杨弘华,严章雪 and 汪波   

  • Received:2015-11-19 Revised:2016-01-12 Accepted:2016-01-22 Online:2016-04-20 Published:2016-04-20

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摘要： 为提高香菇多糖提取效率、研究其抗氧化性和抑菌效果,采用超声波辅助热水浸提法,设计L9(33)正交试验在料液比、浸提温度和超声时间三个因素优化香菇多糖提取工艺,检测其提取率,抗氧化性和抑菌效果。结果表明：提取多糖效率最佳工艺为料液比1：40(g：mL),浸提温度90℃,超声时间40min,最高提取率达到6.47%。抑菌功效最佳工艺为料液比1：40(g：mL),浸提温度80℃,超声时间20min,大肠杆菌最大抑菌圈直径为9.95±0.86mm,枯草芽孢杆菌最大抑菌圈直径为8.73±0.57mm。清除羟基最佳工艺料液比1：30(g：mL),浸提温度90℃,超声时间30min,清除率为22.04%。还原力最佳工艺料液比1：30(g：mL),浸提温度90℃,超声时间40min,其还原力最大。在提取和抗氧化试验中的三个因素影响程度相同即料液比＞浸提温度＞超声时间。提取条件进行优化后,提高了提取效率,试验结果还表明,香菇多糖有一定的抑菌和抗氧化性功效。

关键词: 脱硫石膏, 脱硫石膏, 养分, 重金属, 盐碱地

Abstract: To improve the extraction yield of lentinan and study its antioxidant and antibacterial activity, lentinan was extracted using hot water assisted by ultrasonic. The L9(33) orthogonal test was designed to optimized the extraction condition and the influencing factors were solid-liquid ratio, extraction temperature and ultrasonic time. The results showed that the optimum extraction condition was extracting using 90℃ water assisted by ultrasonic for 40 min with the solid-liquid ratio being 1:40. Under this condition, the extraction yield of lentinan was 6.47%. When extracting lentinan using 80℃ water assisted by ultrasonic for 20 min with the solid-liquid ratio being 1:40, the antibacterial activity was the strongest, the inhibition zone diameter of Escherichia coli was (9.95± 0.86) mm, and that of Bacillus subtilis was (8.73± 0.57) mm. When extracting lentinan using 90℃ water assisted by ultrasonic for 30 min with the solid-liquid ratio being 1:30, scavenging activity of hydroxyl radicals reached the maximum of 22.04%. When extracting lentinan using 90℃ water assisted by ultrasonic for 40 min with the solid- liquid ratio being 1:30, the antioxidant activity was the strongest. The influencing degree of the three factors was in an order of solid- liquid ratio ＞ extraction temperature ＞ ultrasonic time. The results indicated that the optimal extraction condition could improve the extraction yield of lentinan, and lentinan had antibacterial and antioxidant activity to some extent.