油菜田看麦娘对精喹禾灵抗性监测及与烯草酮交互抗性

doi:10.11924/j.issn.1000-6850.casb15120093

中国农学通报 ›› 2016, Vol. 32 ›› Issue (18): 170-175.doi: 10.11924/j.issn.1000-6850.casb15120093

所属专题：油料作物；园艺；农业生态

油菜田看麦娘对精喹禾灵抗性监测及与烯草酮交互抗性

唐子慧¹,周小毛²

(¹湖南农业大学农药研究所,长沙 410128；²湖南省农业生物技术研究中心,长沙 410125）

收稿日期:2015-12-15 修回日期:2016-06-13 接受日期:2016-02-25 出版日期:2016-06-28 发布日期:2016-06-28
通讯作者: 周小毛
基金资助:
国家公益性行业（农业）科研专项“杂草抗药性监测及治理技术研究与示范”(201303031)。

Resistance Monitoring of Alopecurus aequalis Sobol. to Quizalofop-P-ethyl and Cross-resistance with Clethodim in Rape Field

Tang Zihui¹, Zhou Xiaomao²

(¹Institute of Pesticides, Hunan Agricultural University, Changsha 410128;²Hunan Agricultural Biotechnology Research Center, Changsha 410125)

Received:2015-12-15 Revised:2016-06-13 Accepted:2016-02-25 Online:2016-06-28 Published:2016-06-28

摘要/Abstract

摘要： 通过对看麦娘抗药性水平的检测以及抗性机理的研究，以期为油菜田看麦娘的抗性治理和科学合理用药提供理论依据。采用整株法测定了长江中下游地区的4个省13个市17个地区的油菜田看麦娘生物型对精喹禾灵的抗性水平及与烯草酮的交互抗性，分别测定了精喹禾灵与烯草酮对抗性看麦娘(R-TY)、敏感看麦娘(S-NJ)体内GST_S活性。抗精喹禾灵监测结果显示：TY生物型相对抗性倍数最高，为28.92，GR₅₀为90.183。其次为YZ生物型和BLD生物型，GR₅₀分别为34.480和45.472，相对抗性倍数分别为11.06和14.58。其余生物型相对抗性倍数在1.62~9.16之间。与烯草酮的交互抗性结果显示：对精喹禾灵产生抗性的TY、BLD生物型对烯草酮也产生了抗性，存在交互抗性，而对精喹禾灵敏感的DYX生物型看麦娘却对烯草酮产生了抗性，说明看麦娘生物型中存在不同的抗性模式。GST_S活性测定结果显示：经药剂处理后，R-TY生物型GST_S活性始终比敏感生物型S-NJ高，表明TY生物型产生抗性的原因可能是GST_S对药剂的代谢作用增强。

关键词: 石漠化, 石漠化, 功能群, 灌木层

Abstract: By detecting the resistance level of Alopecurus aequalis Sobol., and studying its resistance mechanism, a theoretical basis for rational drug use can be provided for A.aequalis resistance management in rape field. The resistance levels of Alopecurus aequalis Sobol. biotypes to quizalofop-P-ethyl and the cross-resistance with clethodim in 17 areas in the middle and lower reaches of Yangtze River were determined using the whole-plant assay method. The glutathione-s-transferase (GST_S) enzyme activity was studied in resistant biotype (R-TY) and susceptible biotype (S-NJ) with quizalofop-P-ethyl and clethodim. The anti-quizalofop monitoring results showed that: TY biotype had the highest relative resistance multiple, which was 28.92, GR₅₀ was 90.183. And then were YZ and BLD biotype, of which, GR₅₀ was 34.480 and 45.472, respectively, and the relative resistance multiple was 11.06 and 14.58, respectively. The relative resistance multiples of other biotypes were ranged from 1.62 to 9.16. The cross-resistance with clethodim showed that there was a cross-resistance between quizalofop and clethodim in TY and BLD biotype, while DYX, the quizalofop sensitive biotype, was resistant to clethodim, indicating A. aequalis biotypes might have other resistance patterns. GST_S activity assay showed that: after drug treatment, the GST_S activity in R-TY biotype was always higher than that in the sensitive biotype S-NJ, indicating that TY biotype’s resistance to drug might be due to the GST_S enhancing effect on drug metabolism.

唐子慧,周小毛. 油菜田看麦娘对精喹禾灵抗性监测及与烯草酮交互抗性[J]. 中国农学通报, 2016, 32(18): 170-175.

Tang Zihui and Zhou Xiaomao. Resistance Monitoring of Alopecurus aequalis Sobol. to Quizalofop-P-ethyl and Cross-resistance with Clethodim in Rape Field[J]. Chinese Agricultural Science Bulletin, 2016, 32(18): 170-175.

参考文献

[24] 祝玮玮,刘艺妩,刘祥英,等.油菜田看麦娘对精喹禾灵的抗性水平及抗性机理研究[J].植物保护,2014,40(5):80-85.
[25] Herbicide Resistance Action Committee [EB/OL]. [2013-08-03]. http://www.hracglobal.com.
[26] 马晓渊.农田杂草抗药性的发生为害、原因与治理[J].杂草科学,2002(1):5-9.
[27] Devine M D. Mechanisms of resistance to acetyl-coenzyme A carboxylase inhibitors: a review [J]. Pesticide Science, 1997,51(3):259-264.
[28] Christophye D. Weed resistance to Acetyl-coenzyme A carboxylase inhibitors: an update [J]. Weed Science, 2005, 53: 728-746.
[29] Cummins I, Wortley D J, Sabbadin F, et al. Key role for a glutathione transferase in multiple-herbicide resistance in grass weeds[J]. Proceedings of the National Academy of Sciences, 2013, 110(15): 5812-5817.
[30] Cummins I, Moss S, Cole D J, et al. Glutathione transferases in herbicide‐resistant and herbicide‐susceptible black-grass (Alopecurus myosuroides)[J]. Pesticide science, 1997, 51(3):244-250.[1] 王汉中,官春云,张春雷.中国的油菜生产与栽培科学技术研究[A].第十二届国际油菜大会论文集[C].2007:2-7.
[2] 苏少泉,宋顺祖.中国农田杂草化学防治[M].北京:中国农业出版社,1996.
[3] 孙炳耀.麦田看麦娘属杂草种群消长原因及其抗药性鉴定的研究[D]. 南京:南京农业大学,1996,
[4] 赵玉信,杨惠敏.物格局、土壤耕作和水肥管理对农田杂草发生的影响及其调控机制[J].草业学报,2015,24(8):199-210.
[5] 韩敏,李粉华,孙国俊,等.江苏金坛地区小麦田杂草种群生态位研究[J].江西农业学报,2015,27(9):45-49.
[6] 鲁军雄,陈社员,官春云,等.长江中下游油菜田杂草发生规律及实用防治策略[J].作物研究,2012,26(6):639-642.
[7] 赵虎基,王国英.植物乙酷辅酶A羧化酶的分子生物学与基因工程[J].中国生物工程杂志,2003,23(2):12-16.
[8] 黄世霞,王庆亚,董立尧,等.乙酰辅酶Ａ羧化酶抑制剂类除草剂与杂草的抗药性[J].杂草科学,2003(2):1-5.
[9] Zhang X Q, Powles B S. The Molecular Bases for Resistance to Acetyl-coenzyme A Carboxylase (ACCase) Inhibiting Herbicides in Two Target-based Resistant Biotypes of Annual Ryegrass(Lolium rigidum)[J]. Planta, 2006, 223: 550-557.
[10] Marles M A S, Devine M D, Hall J C. Herbicide resistance in Setaria viridis conferred by a less sensitive form of acetyl coenzyme A carboxylase[J]. Pesticide Biochemistry and Physiology, 1993, 46(1): 7-14.
[11] Devine M D. Mechanisms of resistance to acetyl-coenzyme A carboxylase inhibitors: a review[J]. Pesticide Science, 1997, 51(3): 259-264.
[12] Moss S R, Cussans G W. The development of herbicide resistant populations of Alopecurus myosuroides (black-grass) in England[J]. Herbicide resistance in weeds and crops, 1991: 45-55.
[13] 黄世霞,王庆亚,张守栋.油菜田看麦娘对精喹禾灵和烯禾啶交互抗性[J].农药,2008,47(9):679-681.
[14] Guo W, Liu W, Li L, et al. Molecular Basis for Resistance to Fenoxaprop in Shortawn Foxtail (Alopecurus aequalis) from China[J]. Weed Science, 2015, 63(2): 416-424.
[15] Marrs K A. The functions and regulation of glutathione S-transferases in plants[J]. Annual review of plant biology, 1996, 47(1): 127-158.
[16] Neuefeind T, Reinemer P, Bieseler B. Plant glutathione S-transferases and herbicide detoxification[J]. Biological chemistry, 1996, 378(3-4): 199-205.
[17] Timmerman K P. Molecular characterization of corn glutathione S-transferase isozymes involved in herbicide detoxication[J]. Physiologia Plantarum, 1989, 77(3): 465-471.
[18] Hall L M, Moss S R, Powles S B. Mechanisms of resistance to aryloxyphenoxypropionate herbicides in two resistance biotypes of Alopecurus mysouroides (black grass): herbicide metabolism as a cross-resistance mechanism [J]. Pesticide Biochemistry and Physiology, 1997,57(2):87-98.
[19] Czarnota M A, Rimando A M, Weston L A. Evaluation of root exudates of seven sorghum accessions. Journal of Chemical Ecology,2003, 29(9):2073-2083.
[20] 王庆亚,董立尧,娄远来,等.田杂草抗药性及其检测鉴定方法[J].杂草科学,2002(2):1-5.
[21] 张朝贤,倪汉文,魏守辉,等.杂草抗药性研究进展[J].中国农业科学,2009,42(4):1274-1289.
[22] 吴进才,徐俊峰,冯绪猛,等.稻田常用农药对水稻3个品种生理生化的影响[J].中国农业科学,2003,36(5):536-541.
[23] 韩瑞娟.日本看麦娘对高效氟吡甲禾灵抗性机理的研究[D].南京:南京农业大学,2010.
[24] 祝玮玮,刘艺妩,刘祥英,等.油菜田看麦娘对精喹禾灵的抗性水平及抗性机理研究[J].植物保护,2014,40(5):80-85.
[25] Herbicide Resistance Action Committee [EB/OL]. [2013-08-03]. http://www.hracglobal.com.
[26] 马晓渊.农田杂草抗药性的发生为害、原因与治理[J].杂草科学,2002(1):5-9.
[27] Devine M D. Mechanisms of resistance to acetyl-coenzyme A carboxylase inhibitors: a review [J]. Pesticide Science, 1997,51(3):259-264.
[28] Christophye D. Weed resistance to Acetyl-coenzyme A carboxylase inhibitors: an update [J]. Weed Science, 2005, 53: 728-746.
[29] Cummins I, Wortley D J, Sabbadin F, et al. Key role for a glutathione transferase in multiple-herbicide resistance in grass weeds[J]. Proceedings of the National Academy of Sciences, 2013, 110(15): 5812-5817.
[30] Cummins I, Moss S, Cole D J, et al. Glutathione transferases in herbicide resistant and herbicide susceptible black-grass (Alopecurus myosuroides)[J]. Pesticide science, 1997, 51(3):244-250.

油菜田看麦娘对精喹禾灵抗性监测及与烯草酮交互抗性

Resistance Monitoring of Alopecurus aequalis Sobol. to Quizalofop-P-ethyl and Cross-resistance with Clethodim in Rape Field

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