关键词: 西伯利亚鲟, 西伯利亚鲟, 美洲鲥鱼, 暗纹东方鲀, 精液, 超低温保存

Abstract: The application status of fertilizer and pesticide of open field, greenhouse and solar greenhouse in eight agricultural counties of Xi’an (total 422 samples) was investigated. The results showed that the average application amounts of nitrogen, phosphorus and potassium fertilizer were N 327.0 kg/hm2, P2O5 186.0 kg/hm2 and K2O 138.0 kg/hm2 respectively in open vegetable field, the dosage of nitrogen and phosphorus fertilizer was too much and the dosage of potassium fertilizer was suitable. The average application amounts of nitrogen, phosphorus and potassium fertilizer were N 868.5 kg/hm2, P2O5 544.5 kg/hm2 and K2O 496.5 kg/hm2 respectively in greenhouse vegetable field, and that of solar greenhouse were N 883.5 kg/hm2, P2O5 684.0 kg/hm2 and K2O 724.5 kg/hm2 respectively, the chemical fertilizer application amount in greenhouse vegetable field was too much, especially the application amount of phosphate fertilizer. The application amount of chemical fertilizer should be controlled strictly according to soil testing and formulated fertilization in vegetable field, especially in greenhouse vegetable field, the application ration of nitrogen, phosphorus and potassium fertilizer should be coordinated. The application amount of nitrogen, phosphorus and potassium fertilizer in open vegetable field increased with the increase of planting years, while the relationship was not significant in greenhouse and solar greenhouse; the dosage of vegetable field pesticide and open filed pesticide was suitable, but the dosage of pesticide in greenhouse vegetable field, especially solar greenhouse was too much. The dosage of pesticide showed an increasing trend with the increase of planting years. The increasing trend of pesticide application amount should be restrained by the scientific application of pesticide, thereby to solve the problem of agroecological environment pollution caused by abuse application of pesticide.