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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (5): 55-58.doi: 10.11924/j.issn.1000-6850.2014-2464

所属专题： 水产渔业

• 水产 渔业 • 上一篇    下一篇

3种长江珍稀鱼类精子超低温冷冻保存的初步研究

王明华1,钟立强1,陈友明1,丁淑燕1,秦 钦1,边文冀1,蔡永祥2,陈校辉1   

  1. (1江苏省淡水水产研究所,南京 210017；2江苏省海洋水产研究所,江苏南通 226007）
  • 收稿日期:2014-09-11 修回日期:2015-02-02 接受日期:2014-10-30 出版日期:2015-03-20 发布日期:2015-03-20
  • 通讯作者: 王明华
  • 基金资助:
    国家星火计划项目“长江洄游珍稀鱼类产业化关键技术集成”（2012GA690001）。

Study on Sperm Cryopreservation of Three Rare Migratory Fishes in the Yangtze River

Wang Minghua1, Zong Liqiang1, Chen Youming1, Ding Shuyan1, Qin Qin1,   

  1. Bian Wenji1, Cai Yongxiang2, Chen Xiaohui1（1Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210017; 2Institute of Oceanology & marine Fisheries, Nantong jiangsu 226007)
  • Received:2014-09-11 Revised:2015-02-02 Accepted:2014-10-30 Online:2015-03-20 Published:2015-03-20

摘要： 试验对西伯利亚鲟、美洲鲥鱼和暗纹东方鲀3种长江珍稀鱼类的精液进行超低温冷冻保存技术研究。以解冻后精子的活力为参数，探讨了稀释液、不同种类不同体积分数的保护剂、以及不同的精液稀释比例对实验鱼类精液进行超低温冷冻保存的保护效果。结果显示，3种长江珍稀鱼类以配方2和体积分数为10%甲醇组成抗冻保护剂，西伯利亚鲟精液按1:2稀释倍数，保存10天后检查精子活力，存活率可达(45±5.00)%。暗纹东方鲀精液按1:2稀释倍数，保存20天后检查精子活力，存活率可达(60.5±2.69)%。美洲鲥鱼精液按1:3稀释倍数，保存60天后检查精子活力，存活率可达(80.3±2.58)%。3种珍稀鱼类的精子保存以美洲鲥鱼效果最好。保存方法均采用三步法，在液氮面上方6 cm处平衡10 min，接着在液氮面上平衡5 min，最后投入液氮中冷冻保存。研究结果为保护长江珍稀鱼类的种质资源提供了理论基础。

关键词: 花生, 花生, 断根, 植株生长, 开花

Abstract: The technique of sperm cryopreservation of three rare migratory fishes in the Yangtze River was studied, including Acipenser baerii, Alosa sapidissima and Takifugu obscurus. The success of sperm cryopreservation was evaluated by post-thaw sperm motility. To explore methods for sperm cryopreservation, this study evaluated the effects of different extenders, cryoprotectants, their volume fractions, equilibration time and dilution ratios. The result showed that the best sperm cryopreservation of motility of Acipenser baerii was (45±5.00)% and Takifugu obscurus (60.5±2.69)% were obtained by following procedure: the ringer solution and the semen were mixed at ratio of 2:1 in volume and added 10% methanol, while the best sperm cryopreservation of motility of Alosa sapidissima (80.3±2.58)% was obtained by following procedure: the ringer solution and the semen were mixed at ratio of 3:1 in volume and added 10% methanol. Sperms were cryopreserved by three-step cooling progam. These results provided the methods to conserve genetic resources in three rare migratory fishes in the Yangtze River.