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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (20): 6-10.doi: 10.11924/j.issn.1000-6850.2014-0562

所属专题： 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

酉州乌羊精液品质评定及稀释液配方研究

赵金红   

  • 收稿日期:2014-03-06 修回日期:2014-04-09 出版日期:2014-07-15 发布日期:2014-07-15
  • 基金资助:
    重庆市科委重点攻关项目“酉州乌羊幼畜胚胎体外生产技术研究与示”（CSTC,2011AB1084）；重庆市基本科研业务费专项“山羊精液冷冻保存研究”（12604）；重庆市工程技术研究中心建设项目“重庆市山羊工程技术研究中心”（cstc2011pt-gc80014）。

Study on The Quality of Ram Semen and Extender for Youzhou Black-skinned Goat Semen

  • Received:2014-03-06 Revised:2014-04-09 Online:2014-07-15 Published:2014-07-15

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摘要： 为了保护和利用酉州乌羊种质资源，作者对酉州乌羊种公羊的精液品质进行了评定，并试验筛选适合酉州乌羊精液的优良稀释液配方。研究结果表明，酉州乌羊射精量为0.85 mL、活力为81.56%、精子畸形率为7.27%、顶体完整率为91.48%、精子密度为23.8亿/mL，均属正常范围，符合GB20557- 2006山羊精液标准；Ⅲ号精液稀释液保存效果显著优于其它稀释液配方（P＜0.05），其低温环境保存精液效果明显好于常温和高温。结论：保护场内酉州乌羊种公羊的精液品质较好，可以进行正常的配种和使用，稀释液Ⅲ号适用于精液保存。

关键词: 现状, 现状

Abstract: To protect and utilize a precious genetic resource of Youzhou black- skinned Goat, the author conducted experiments to investigate the quality of ram semen. Several semen extenders were studied in order to find out which is the best extender. The results showed that he average ejaculate volume was 0.85 mL, vitality was 81.56% and the rate of terato sperm was 7.27 %, intact acrosome rate was 91.48 %, sperm density was 23.8 billion/mL, all the index met the requirements of GB20557- 2006. The effects of the ram’s spermatozoa conserved inⅢdiluent was better than other diluents，(P＜0.05)．and the effects of the ram’s spermatozoa conserved in low temperature environment is better than in room temperature environment and high temperature. In conclusion, the collected fresh semen of rams in Conservation field of Chongqing city Youyang was found to be of good quality, the rams are capable of hybridization, the diluentⅢcould be used to conserve ram semen .