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中国农学通报

中国农学通报 ›› 2016, Vol. 32 ›› Issue (31): 85-89.doi: 10.11924/j.issn.1000-6850.casb16070066

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

60Co-γ辐射石斛兰组培苗的SRAP分析

任羽,潘丙成,陆顺教,张志群,孔霞   

  1. 中国热带农业科学院热带作物品种资源研究所,中国热带农业科学院热带作物品种资源研究所,中国热带农业科学院热带作物品种资源研究所,中国热带农业科学院热带作物品种资源研究所,中国热带农业科学院热带作物品种资源研究所
  • 收稿日期:2016-07-13 修回日期:2016-10-10 接受日期:2016-09-20 出版日期:2016-11-10 发布日期:2016-11-10
  • 通讯作者: 任羽
  • 基金资助:
    ：国家星火计划项目“石斛兰新品种种苗繁育及配套栽培技术示范推广”(2015GA800008)；中央级公益性科研院所基本科研业务费专项“石 斛兰遗传图谱构建”(1630032015023)；海南省应用技术研发与示范推广专项“海南钻喙兰、密花石斛、竹叶兰高效栽培技术研究与示范” (ZDXM2014110)。

SRAP Analysis of Dendrobium Tissue Culture Seedlings Based on 60Coγ-ray Radiation

  • Received:2016-07-13 Revised:2016-10-10 Accepted:2016-09-20 Online:2016-11-10 Published:2016-11-10

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摘要： 为研究石斛兰辐射诱变后的植株性状遗传变异程度,本研究利用SRAP分子标记对不同剂量60Coγ射线处理后的石斛兰D.Sonia‘166’品种组培苗进行分析,探讨其辐射诱变效应,确定最佳的辐射剂量。结果表明：利用从100对引物中筛选出的12对引物对不同剂量处理的石斛兰基因组DNA进行扩增,共扩增出了216条带,其中多态性带41条,多态率为19.0%。对不同处理及对照扩增的谱带进行聚类分析发现,各个剂量的辐照处理群体都发生了不同程度的变异,20Gy处理整体变异程度最大,5Gy变异最小。因此,认为石斛兰D.Sonia‘166’品种组培苗的最佳辐射诱变剂量为20 Gy。本文为辐射诱变育种和诱变后代的选择提供理论依据。

关键词: 玉米, 玉米, 分期播种, 气候变化, 生长速度, 产量, 辽西地区

Abstract: To study the genetic variation degree of plant characters of Dendrobium tissue culture seedlings treated by radiation mutation, in this study, SRAP molecular markers technique was used to analyze Dendrobium sonia‘166’variety tissue culture seedlings treated by different doses of 60Coγ ray. The radiation mutagenesis effect was discussed, and the optimal irradiation dosage was determined. The results showed that 12 primers screened out from 100 pairs primers were used to amplify the genomic DNA of Dendrobium treated with different doses of 60Coγ ray. The 216 bands were amplified, among them 41 bands were polymorphic with the ratio of 19.0%. The cluster analysis of the bands amplified in treated and controlled plants showed that D. sonia‘166’varieties tissue culture seedlings treated by different doses of 60Coγ ray mutated in different extent. The variation degree was maximum in 20 Gy treated plants, and was minimum in 5 Gy treated plants. Therefore 20 Gy was the best radiation dose of the 60Coγ-ray for variation induction.