液质联用法分离鉴定甜叶菊叶片中的不同甜菊糖苷

doi:10.11924/j.issn.1000-6850.casb16090028

中国农学通报 ›› 2017, Vol. 33 ›› Issue (30): 43-50.doi: 10.11924/j.issn.1000-6850.casb16090028

液质联用法分离鉴定甜叶菊叶片中的不同甜菊糖苷

朱静雯,郭书巧,束红梅,巩元勇,蒋璐,倪万潮

江苏省农业科学院经济作物研究所,江苏省农业科学院经济作物研究所,江苏省农业科学院经济作物研究所,江苏省农业科学院经济作物研究所,江苏省农业科学院经济作物研究所,江苏省农业科学院经济作物研究所

收稿日期:2016-09-06 修回日期:2016-11-09 接受日期:2016-11-25 出版日期:2017-10-31 发布日期:2017-10-31
通讯作者: 倪万潮
基金资助:
江苏省农业三新工程“甜叶菊新品种高产高效栽培技术示范推广”；江苏省农业科学院科技服务项目“甜叶菊新品种高产高效栽培技术示范推广”

Identification and Analysis of Steviol Glycosides in Stevia Rebaudiana Leaves by HPLC-MS

Received:2016-09-06 Revised:2016-11-09 Accepted:2016-11-25 Online:2017-10-31 Published:2017-10-31

摘要/Abstract

摘要： 为了进一步完善甜叶菊叶片中不同甜菊糖苷的测定方法，采用液质联用技术对多种甜菊糖苷标准品和‘鑫丰3号’甜叶菊叶片粗提物进行分析测定。最终共确定12种甜菊糖苷的出峰时间和顺序，其中甜茶苷、甜菊糖双苷和莱鲍迪苷E的出峰时间和顺序为首次确定。基于莱鲍迪苷A的测定标准曲线和多种甜菊糖苷的高效液相检测图谱结果确定‘鑫丰3号’叶片中的甜菊糖苷组成。‘鑫丰3号’叶片中共含有甜菊苷，莱鲍迪苷A、B、C、D、E和莱鲍迪苷F，甜茶苷，甜菊糖双苷9种甜菊糖苷，其中莱鲍迪苷D和E为痕量存在。本研究不仅为后续甜菊糖苷的测定提供了目前已有报道中最全面的紫外检测图谱依据同时也为新类型甜菊糖苷的发现和鉴定提供了方法参考。

关键词: 地膜覆盖方式, 地膜覆盖方式, 小气候, 黄瓜, 生长, 品质

Abstract: To improve the measurement method of steviol glycosides (SGs) in Stevia leaves, several SGs standards and leaf extracts of‘Xinfeng 3’were analyzed and determined by using high performance liquid chromatography-mass spectrometer (HPLC-MS). And the retention time and order of 12 different SGs were identified, among which three (rubusoside, steviolbioside and rebaudioside E) were first reported. Based on the rebaudiodside A standard curve and HPLC fingerprints map of SGs, the composition of SGs in‘Xinfeng 3’ leaves was determined. Totally nine kinds of SGs including stevioside, rebaudioside A, B, C, D, E and F, rubusoside and steviolbioside were identified. The rebaudioside D and E were presented in trace amount. This study provides not only detailed ultraviolet-map for further detection of SGs, but also a reference method for finding and identifying new SGs.

朱静雯,郭书巧,束红梅,巩元勇,蒋璐,倪万潮. 液质联用法分离鉴定甜叶菊叶片中的不同甜菊糖苷[J]. 中国农学通报, 2017, 33(30): 43-50.

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液质联用法分离鉴定甜叶菊叶片中的不同甜菊糖苷

Identification and Analysis of Steviol Glycosides in Stevia Rebaudiana Leaves by HPLC-MS

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