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中国农学通报

中国农学通报 ›› 2017, Vol. 33 ›› Issue (35): 46-49.doi: 10.11924/j.issn.1000-6850.casb16120149

• 生物技术科学 • 上一篇    下一篇

香茅精油不同接触方式对体外白假丝酵母菌抑制效果的研究

李珊珊,林霜霜,杨 敏,黄惠明,吴维坚   

  1. 福建省农科院亚热带农业研究所,福建省农科院亚热带农业研究所,福建省农科院亚热带农业研究所,福建省农科院亚热带农业研究所,福建省农科院亚热带农业研究所
  • 收稿日期:2016-12-30 修回日期:2017-11-24 接受日期:2017-04-19 出版日期:2017-12-14 发布日期:2017-12-14
  • 通讯作者: 吴维坚
  • 基金资助:
    ：福建省科技厅公益类科研院所专项“香茅精油对蚊虫的趋避效果极其配方研究”(2016R1012-7)；福建省科技厅公益类科研院所专项“芳香 植物天然产物提取工艺及成分分析”(2015R1013-4)。

Inhibitory Effect of Different Contact Methods of Citronella Oil on Candida albicans

黄惠明 and   

  • Received:2016-12-30 Revised:2017-11-24 Accepted:2017-04-19 Online:2017-12-14 Published:2017-12-14

摘要： 采用漳州地区种植的柠檬香茅草，提取其精油分别用不同物态定量抑制白假丝酵母菌，以此筛选出该精油抑制该菌种的最佳物态及最低浓度；采用固态直接接触法、气态熏蒸法和液态混合药剂法筛选出柠檬香茅精油对供试菌种的最佳抑制物态，并在此基础上筛选出该精油抑制供试菌种的最低浓度。结果表明用固态直接接触法的抑菌效果最佳，气态熏蒸法次之，液态混合药剂法的抑菌效果最差，但仍显著优于5%氟康唑阳性对照(P＜0.01)，柠檬香茅精油固态直接接触法抑制白假丝酵母菌的最低浓度为0.15 μL/mL。本实验可证明柠檬香茅精油在三种物态下都有良好的抑菌效果。

关键词: 添加物, 添加物, 干旱, 高粱, 种子萌发

Abstract: Using the lemongrass (Cymbopogon citratus) which grown in Zhangzhou area, the essential oil is extracted to quantitatively inhibit Candida albicans in different forms, in order to screen the optimum form and the minimum concentration of this essential oil against C.albicans. In the study, the optimum form of inhibition were carried out by three forms of solid, gas and liquid, and the minimum concentration of the essential oil was tested. The qualitative experiment result showed that the essential oil of lemongrass had the best antimicrobial ability for C. albicans in solid state, and liquid state had the worst activity but was still significantly better than 5% fluconazole positive control (P＜0.01). In the quantitative experiment, the minimum inhibitory concentration (MIC) of Candida albicans was 0. 15μL/mL in solid state. This experiment proved that the essential oil of lemongrass had the antimicrobial ability for Candida albicans in all three forms.