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中国农学通报 ›› 2017, Vol. 33 ›› Issue (36): 85-92.doi: 10.11924/j.issn.1000-6850.casb17040121

• 林学 园艺 园林 • 上一篇    下一篇

大花萱草‘黄油花’和‘粉秀客’杂交子代的鉴定与核型分析

李金霞,储博彦,赵玉芬,尹新彦   

  1. 河北省林业科学研究院,河北省林木良种工程技术研究中心;河北省林业科学研究院,河北省林木良种工程技术研究中心;河北省林业科学研究院,河北省林木良种工程技术研究中心;河北省林业科学研究院
  • 收稿日期:2017-04-19 修回日期:2017-12-20 接受日期:2017-07-21 出版日期:2017-12-29 发布日期:2017-12-29
  • 通讯作者: 李金霞
  • 基金资助:
    河北省林业科学技术研究项目“大花萱草杂交育种”(1208432)。

Hemerocallis hybrida; H.‘Gleber’s Top Gream’; H.‘Pink silk ruffle’; hybrids; identification; karyotype analysis

  • Received:2017-04-19 Revised:2017-12-20 Accepted:2017-07-21 Online:2017-12-29 Published:2017-12-29

摘要: 为获得具有优良观赏性状的萱草新品种,以大花萱草‘黄油花’(H.‘Gleber’s Top Gream’)为母本,‘粉秀客’( H.‘Pink silk ruffle’)为父本进行杂交,获得了一些发生性状分离的杂交后代,对后代株系中性状较稳定的4个株系综合利用形态学观察和核型分析进行了杂种鉴定。结果表明:杂交后代遗传了父母本的形态学特征;双亲及其4个杂交后代均为二倍体(2n=2x=22),后代核型特征多介于父母本之间,以2B型为主。从形态学、细胞学方面证明杂交后代整合了亲本的遗传物质和表型特征,确定为真杂种,为利用大花萱草选育新品种提供了新的种质材料。

关键词: N肥, N肥, 硒肥, 裸燕麦, 产量, 品质, 硒肥效率

Abstract: To get new varieties of Hemerocallis with excellent ornamental characteristics,H.‘Gleber’s Top Gream’(as female parent) and H.‘Pink silk ruffle’(as male parent) were crossed and some hybrids were obtained. 4 hybrids with stable characteristics were selected. Hybrid identification was conducted with morphological observation and karyotype analysis. The results showed that the 4 hybrids obtained the morphological characteristics of both female parent and male parent, the parents and the 4 hybrids were all diploids (2n = 2x = 22), the most karyotype parameters of the 4 hybrids were between their parents, the karyotype of most hybrids were 2B. The morphological and cytological characteristics proved that hybrids integrated the genetic material and morphological traits from their parents, and were identified as true hybrids, which provided new germplasm materials for selecting new varieties of Hemerocallis hybrida.