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中国农学通报 ›› 2017, Vol. 33 ›› Issue (28): 26-31.doi: 10.11924/j.issn.1000-6850.casb17060053

所属专题: 烟草种植与生产 农业生态

• 农学 农业基础科学 • 上一篇    下一篇

喷施亚油酸对烟草胁迫抗性的影响研究

孙慧,韩小斌,夏志林,王小彦,石永春   

  1. 河南农业大学生命科学学院,贵州省烟草公司遵义市公司,贵州省烟草公司遵义市公司,贵州省烟草公司遵义市公司,河南农业大学生命科学学院
  • 收稿日期:2017-06-08 修回日期:2017-09-27 接受日期:2017-08-09 出版日期:2017-10-24 发布日期:2017-10-24
  • 通讯作者: 王小彦
  • 基金资助:
    贵州省烟草公司遵义市公司项目(201506):烟叶抗病剂对烟草普通花叶病抗性影响研究与应用。

Effects of Spraying Linoleic Acid on Stress Resistance of Tobacco

  • Received:2017-06-08 Revised:2017-09-27 Accepted:2017-08-09 Online:2017-10-24 Published:2017-10-24

摘要: 为了探讨亚油酸能否提高烟草的胁迫抗性,检测了不同浓度的亚油酸喷施烟草后,叶片中抗氧化酶活性,活性氧、MDA、渗透调节物质的含量,以及抗逆和抗病相关基因的表达水平。结果显示,亚油酸处理降低了烟叶中的MDA含量,提高了脯氨酸、可溶性糖、H2O2含量和POD活性,增强了rbohD、rbohF、WRKY57、PR-1a、JAZ1和APX基因的表达水平,且在10mM时值最高。表明亚油酸能增强烟叶对胁迫的耐受性,且在10 mM亚油酸处理时效果最佳。

关键词: 监测, 监测, 部署, 苹果园, 微气候, 冠层

Abstract: To investigate whether linoleic acid could enhance the stress resistance of tobacco, different concentrations of linoleic acid were sprayed to tobacco. The activities of antioxidant enzymes and the contents of proline, soluble sugar, H2O2 and MDA were detected. The expression levels of genes related to stress resistance were also detected by qPCR. Results showed that linoleic acid reduced the contents of MDA in tobacco leaves, but increased the content of proline, soluble sugar, H2O2 and POD activity. The expression levels of rbohD, rbohF, WRKY57, PR-1a, JAZ1 and APX were also increased, especially at 10 mM concentration. Suggesting that linoleic acid enhanced stress tolerance of tobacco. Moreover, the best effects were got at 10 mM linoleic acid treatment.