Abstract: To screen out good varieties which are suitable for planting in Guizhou Province, 8 new materials of Coix lacryma- jobi including Y10, ZY, Y42, QL, Y43, Y8, Y160 and ZF, were used as materials. ‘Xingrenxiaobaike’was taken as the control. The genetic relationship among the 8 materials was identified by SSR markers. Variety comparison test was conducted with randomized block design. Results showed that the variation range of genetic similarity coefficients was between 0.467 and 0.922 by cluster analysis on amplified sequence by 20 pairs of combined SSR primers, and there were differences among test materials which demonstrated they were different test materials. Based on the variety comparison analysis between 2015 and 2016, yields of Y10, ZY, Y42, QL, Y160 reached very significant level compare to the control, and the yield of Y10 reached the highest in both the two years. The test demonstrates that the new materials Y10, ZY, Y42, QL and Y160 have significant advantages, and lays a foundation for further research.