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中国农学通报 ›› 2018, Vol. 34 ›› Issue (4): 143-146.doi: 10.11924/j.issn.1000-6850.casb17090145

所属专题: 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

三种鸭源细小病毒的抗原相关性研究

程晓霞.,朱小丽.,林锋强.,王劭.,陈仕龙.,肖世峰.,陈少莺.,俞伏松   

  1. 福建省农业科学院畜牧兽医研究所,福建省农业科学院畜牧兽医研究所,福建省农业科学院畜牧兽医研究所,福建省农业科学院畜牧兽医研究所,福建省农业科学院畜牧兽医研究所,福建省农业科学院畜牧兽医研究所,福建省农业科学院畜牧兽医研究所,福建省畜禽疫病防治工程技术研究中心
  • 收稿日期:2017-09-27 修回日期:2018-01-31 接受日期:2017-12-22 出版日期:2018-02-26 发布日期:2018-02-26
  • 通讯作者: 陈少莺.
  • 基金资助:
    国家重点研发计划“鸭源小鹅瘟胶乳检测试剂盒”(2016YFD0500805-4);福州市科技项目“雏番鸭细小病毒病和小鹅瘟病二联活疫苗产业 化关键技术”(2016-G-41);福建省公益类科研院所专项“短嘴型小鹅瘟病毒单克隆抗体的制备及应用研究”(2016R1022-5)。

Study on Antigenic Correlation of Three Duck Origin Parvovirus

  • Received:2017-09-27 Revised:2018-01-31 Accepted:2017-12-22 Online:2018-02-26 Published:2018-02-26

摘要: 为了明确番鸭细小病毒(MPV F株)、番鸭源鹅细小病毒(MD-GPV PT株)和鸭短喙型鹅细小病毒(SBDS-GPV M15株)的抗原相关性,本研究应用微量中和试验(NT)和胶乳凝集抑制试验(LPAI)测定了三种病毒分别与同源和异源血清的中和抗体和LPAI抗体效价,分析三者之间的抗原相关性。结果显示:3种病毒与同源抗血清的中和效价和LPAI效价均明显高于异源血清;MPV与MD-GPV和SBDS-GPV之间的中和抗体效价R值分别为0.01和0.02,LPAI抗体效价R值分别为0.03和0.04;MD-GPV和SBDS-GPV的中和抗体和LPAI抗体效价R值均为0.35。表明MPV与GPV(包括MD-GPV和SBDS-GPV)之间的抗原相关性较低;MD-GPV与SBDS-GPV抗原相关性较高,为同一血清型的不同亚型。研究结果为有效防控不同鸭源细小病毒感染提供了理论依据。

关键词: 高原特色农业, 高原特色农业, 比较优势, 发展空间

Abstract: To define antigenic correlation of muscovy parvovirus (MPV F strains), muscovy duck origin goose parvovirus (MD-GPV PT strain) and duck short beak and dwarfism syndrome goose parvovirus (SBDSV M15 strain), homologous and heterologous neutralization antibody titer and latex agglutination inhibition test (LPAI) antibody titer of the three virus antigen were measured by trace neutralization test (NT) and LPAI, to analyze the antigenic correlation among these three. The results showed that the homologous neutralization titer and LPAI titer of the 3 viruses were significantly higher than that of heterologous titer,R value of the neutralization antibody titer was 0.01 and 0.02 respectively when MPV reacted with MD-GPV and SBDS-GPV antiserum.R values of LPAI antibody titer was 0.03 and 0.04, respectively. However,R value of neutralizing antibodies and LPAI antibody titer between MD-GPV and SBDS-GPV were both 0.35. The results indicated that antigenic correlation was low between MPV and GPV (including MD-GPV and SBDSV), while it was high between MDGPV and SBDSV, which revealed that MD- GPV and SBDSV belonged to different subtypes of the same serotype. The results provide a theory basis for the effective prevention and control of different sources of duck parvovirus infection.