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中国农学通报 ›› 2018, Vol. 34 ›› Issue (24): 64-70.doi: 10.11924/j.issn.1000-6850.casb18040087

• 食品 营养 检测 安全 • 上一篇    下一篇

QuEChERS 结合HPLC-MS/MS 测定 柑橘中5种杀螨剂残留

郭境颖,胡彧娴,赖添财,潘裕添   

  1. 闽南师范大学菌物产业工程技术中心,漳州市农业检验监测中心,漳州市农业检验监测中心,闽南师范大学菌物产业工程技术中心
  • 收稿日期:2018-04-18 修回日期:2018-08-05 接受日期:2018-06-22 出版日期:2018-08-28 发布日期:2018-08-28
  • 通讯作者: 潘裕添
  • 基金资助:
    2018 年漳州市自然科学基金项目“琯溪蜜柚中多残留QuEChERS-LC/MS/MS检测方法的建立及套袋控残技术研究”(2018N02020057)。

Determining Five Acaricide Residues in Citrus by QuEChERS Combining with HPLC-MS/MS

  • Received:2018-04-18 Revised:2018-08-05 Accepted:2018-06-22 Online:2018-08-28 Published:2018-08-28

摘要: 为了监测柑橘中阿维菌素、哒螨灵、螺螨酯、噻螨酮、乙螨唑残留量,应用QuEChERS处理及高液质联用检测技术,研究建立柑橘中上述5 种杀螨剂多残留分析方法。结果表明,琯溪蜜柚、芦柑、脐橙3种柑橘样品经QuEChERS方法处理,净化效果良好。5 种杀螨剂在0.5~500.0 μg/L 范围内线性关系良好(r2均大于0.999),仪器检出限(LOD)为0.01~0.30 μg/kg、定量限(LOQ)为0.05~0.90 μg/kg。加标回收试验,添加阿维菌素、哒螨灵、噻螨酮、乙螨唑为2.0~200.0 μg/kg,螺螨酯为5.0~200.0 μg/kg 时,符合农药残留分析要求。该方法简便快速、灵敏度高、重现性好、检测浓度低,能够满足中国限量标准及多残留检测的要求。

关键词: 烤烟, 烤烟, 气候变化, 敏感性, 脆弱性, 攀西地区

Abstract: To monitor avermectin, pyridaben, spirodiclofen, hexythiazox and etoxazole residues in citrus, a multi- residue analysis method for five acaricide residues in citrus was developed and validated by using QuEChERS processing with HPLC-MS/MS. The results showed that the purification by QuEChERS treatment method was ideal in the three citrus samples of Guanxi pomelo, ponkan and navel orange. The five kinds of acaricides showed a good linear relationship in the range of 0.5-500.0 μg/L (r 2>0.999), the LOD was 0.01- 0.30 μg/kg and the LOQ was 0.05-0.90 μg/kg. The additive recovery test results suggested that the additive concentrations of avermectin, pyridaben, hexythiazox and etoxazole of 2.0-200.0 μg/kg and spirodiclofen of 5.0-200.0 μg/kg were suitable to the requirement of pesticide residue analysis. The method is simple, fast, and has high sensitivity, good reproducibility and low detection concentration, so it can meet the requirements of maximum residue limit (MRL) in China and multi-residue detection.