不同产地滇重楼光合作用能力探究

doi:10.11924/j.issn.1000-6850.casb2020-0828

摘要/Abstract

摘要：

通过对比不同产地滇重楼与相同产地不同来源滇重楼的光合作用能力,研究其生理适应性,以期为滇重楼引种种植及资源保护提供理论基础。利用LI-6800光合作用仪,检测分析不同产地相同品种与相同产地不同来源滇重楼的光合生理的变化模式。结果表明,采自滇西产地滇重楼的光合能力均高于滇东南产地,但差异较大;滇东南产地滇重楼的光合速率差异不大,但其数值低于滇西产地。而不同产地的滇重楼移栽到相同环境下,其叶片气孔导度(Gs)、蒸腾速率(Tr)以及水分利用效率(WUE)值均低于当地品种,而胞间CO₂浓度与环境CO₂浓度的比值(Ci/Ca)则高于当地品种,移栽后的品种光合能力低于当地品种。研究认为,滇西地区所产滇重楼比滇东南地区光合作用能力更强,以大理州永平县滇重楼尤为突出,而移栽后的品种光合能力以及水分利用率低于当地品种。因此在滇重楼的栽培中,应筛选栽培地产的品种进行人工扩大化种植。

关键词: 滇重楼, 产地, 种源, 资源保护, 光合作用, 光响应曲线

Abstract:

To study the adaptability of Pairs polyphylla var. yunnanensis and provide a theoretical basis for its introduction, planting and resource protection, we investigated the photosynthetic capacity of P. polyphylla var. yunnanensis from different growth areas and different sources from the same area. LI-6800 photosynthesis instrument was used to detect the photosynthetic capacity of the same variety from different areas and different sources from the same area. The results show that the photosynthetic capacity of P. polyphylla var. yunnanensis collected from western Yunnan is higher than that from southeastern Yunnan, and the difference is great. There is no significant difference in photosynthetic rate of P. polyphylla var. yunnanensis among different sources in southeastern Yunnan, but the value is lower than that in western Yunnan. However, the leaf stomatal conductance (Gs), transpiration rate (Tr) and water use efficiency (WUE) of P. polyphylla var. yunnanensis from different habitats are lower than those of local varieties, while the ratio of intercellular CO₂ concentration to environmental CO₂ concentration (Ci/Ca) was higher than that of local varieties, and the photosynthetic capacity of transplanted varieties is lower than that of local varieties. The study shows that the photosynthetic capacity of P. polyphylla var. yunnanensis in western Yunnan is stronger than that in southeastern Yunnan, especially in Yongping County of Dali prefecture. However, the photosynthetic capacity and water use efficiency of the transplanted varieties are lower than those of the local varieties. So in the cultivation of Pairs polyphylla var. yunnanensis, the cultivated local varieties should be selected for artificial expansion.

Key words: Pairs polyphylla var. yunnanensis, geographical origin, resource protection, provenance, photosynthesis, light response curve

中图分类号:

R282.2

王翊豪, 李世昌, 刘春路, 赵艳丽, 郑国伟, 徐福荣. 不同产地滇重楼光合作用能力探究[J]. 中国农学通报, 2021, 37(16): 59-64.

Wang Yihao, Li Shichang, Liu Chunlu, Zhao Yanli, Zheng Guowei, Xu Furong. Research on the Photosynthesis Ability of Pairs polyphylla var. yunnanensis from Different Areas[J]. Chinese Agricultural Science Bulletin, 2021, 37(16): 59-64.

图/表 5

参考文献 28

[1]	李恒. 重楼属植物[M]. 北京: 科学出版社, 1998: 36.
[2]	国家药典委员会. 中国药典[M]. 北京: 中国医药科技出版社, 2020: 272.
[3]	高成杰, 刘方炎, 杨文云, 等. 不同海拔下滇重楼叶片与花萼光合特性[J]. 生态学杂志, 2015,34(1).
[4]	陆辉, 许继宏, 陈锐平, 等. 云南重楼属植物资源现状与保护对策[J]. 云南大学学报:自然科学版, 2006(S1):317-320.
[5]	冼康华, 付传明, 苏江, 等. 重楼种子萌发因素分析[J]. 广西科学院学报, 2020,36(1):83-89.
[6]	Liao D, Zhu J, Zhang M, et al. Comparative transcriptomic analysis reveals genes regulating the germination of morphophysiologically dormant Paris polyphylla seeds during a warm stratification[J]. PLoS One, 2019,14(2).
[7]	黄艳萍, 张杰, 杨敏, 等. 接种时期对丛枝菌根真菌侵染的滇重楼幼苗生长发育及甾体皂苷含量的影响[J]. 中草药, 2019(18).
[8]	王骞, 丁媛, 杨敏, 等. 滇重楼品质与产地和表型性状相关性分析[J]. 中国中药杂志, 2019(15):3203-3212.
[9]	Kang Li-ping, Yu Kate, Zhao Yang, et al. Characterization of steroidal glycosides from the extract of Paris polyphylla var. yunnanensis by UPLC/Q-TOF MSE[J]. Journal of Pharmaceutical & Biomedical Analysis, 2012,62:235-249.
[10]	Li-Zhen L, Shu-Dong Z, Fan Z, et al. Transcriptome-Wide Identification and Prediction of miRNAs and Their Targets in Paris polyphylla var. yunnanensis by High-Throughput Sequencing Analysis[J]. International Journal of Molecularences, 2017,18(1):219.
[11]	夏方山, 毛培胜, 闫慧芳, 等. 植物花后光合性能与物质转运的研究进展[J]. 草地学报, 2013,21(3):420-427.
[12]	高成杰, 刘方炎, 王海林, 等. 不同生育期滇重楼光合日变化及光响应特征[J]. 东北林业大学学报, 2015(7).
[13]	Qiang Q, Gao Y, Yu B, et al. Elevated CO₂ enhances growth and differentially affects saponin content in Paris polyphylla var. yunnanensis[J]. Industrial Crops and Products, 2020,147:112-124.
[14]	沈昱翔, 陈朝儒, 张雪妹, 等. 丛枝菌根对滇重楼幼苗生长及光合特性的影响[J]. 作物杂志, 2020(4).
[15]	曹嘉芮, 梁社往, 张勤涛, 等. 不同光强LED蓝光对滇重楼生长、光合特性及皂苷累积的影响[J]. 中国农学通报, 2019,35(14):83-87.
[16]	侯秀丽, 赵峥, 王斌, 等. 滇重楼光合速率日变化及其对生态因子的响应[J]. 江苏农业科学, 2015(7):265-267,356.
[17]	刘杰, 何忠俊. 叶面肥对滇重楼光合的影响[J]. 天津农业科学, 2008,14(5):49-52.
[18]	何忠俊, 杨威, 韦建荣, 等. 氮素形态及其组合对滇重楼产量及有效成分的影响[J]. 云南农业大学学报, 2010,25(1):107-112.
[19]	苏文华, 张光飞. 滇重楼光合作用与环境因子的关系[J]. 云南大学学报:自然科学版, 2003,25(6):545-548.
[20]	张世华, 梁社往, 何忠俊, 等. 10个地理种源滇重楼光合特性的比较研究[J]. 云南农业大学学报:自然科学, 2012(5).
[21]	陈展宇, 张治安, 崔喜艳, 等. 改变源-库关系对人参叶片光合作用日变化的影响[J]. 南京农业大学学报, 2006,29(1):27-30.
[22]	周可金, 肖文娜, 官春云. 不同油菜品种角果光合特性及叶绿素荧光参数的差异[J]. 中国油料作物学报, 2009,31(3):316-321.
[23]	林平, 李吉跃, 陈崇. 银杏光合生理生态特性研究[J]. 北京林业大学学报, 2008,30(6):22-29.
[24]	Qi J, Zheng N, Zhang B, et al. Mining genes involved in the stratification of Paris polyphylla seeds using high-throughput embryo Transcriptome sequencing[J]. BMC Genomics, 2013,14(1):1-14. doi: 10.1186/1471-2164-14-1 URL
[25]	Yang L, Yang Z, Liu C, et al. Chloroplast phylogenomic analysis provides insights into the evolution of the largest eukaryotic genome holder, Paris japonica (Melanthiaceae)[J]. BMC Plant Biology, 2019,19.
[26]	Yang Z, Yang L, Liu C, et al. Transcriptome analyses of Paris polyphylla var. chinensis, Ypsilandra thibetica, and Polygonatum kingianum characterize their steroidal saponin biosynjournal pathway[J]. Fitoterapia, 2019,135:52-63. doi: 10.1016/j.fitote.2019.04.008 URL
[27]	张勤涛, 梁社往, 曹嘉芮, 等. LED不同光质对滇重楼生长,光合特性,皂苷含量及产量的影响[J]. 照明工程学报, 2018,29(4):71-75.
[28]	王旭军, 李琪, 张玉荣, 等. 郁闭林分下华重楼光合特性研究[J]. 湖南林业科技, 2018.

种源编号	样品名称	种源地	北纬	东经	海拔/m	年降雨量/mm	最高温度/℃	最低温度/℃
1	鲁甸高杆	丽江市玉龙县	26°49'24''	100°03'41''	2490	955.80	19.39	7.85
2	华坪高杆	丽江市华坪县	26°43'17''	101°27'29''	2570	1040.33	27.32	13.88
3	永平矮杆	大理州永平县	25°29'21''	99°37'26''	2290	962.64	23.34	10.09
4	马龙高杆	曲靖市马龙区	25°33'20''	103°29'50''	2140	975.35	19.71	9.61
5	曲靖高杆	曲靖市麒麟区	25°15'31''	103°47'22''	2410	961.72	20.89	10.21
6	师宗矮杆	曲靖市师宗县	24°45'03''	103°59'24''	1890	1174.34	20.02	10.05
7	宣威矮杆	曲靖市师宗县	24°45'03''	103°59'24''	1890	1174.34	20.02	10.05
8	罗平高杆	曲靖市师宗县	24°45'03''	103°59'24''	1890	1174.34	20.02	10.05
9	罗平矮杆	曲靖市师宗县	24°45'03''	103°59'24''	1890	1174.34	20.02	10.05
10	泸西矮杆	曲靖市师宗县	24°33'32''	103°47'44''	1810	910.44	21.64	10.71

种源编号	样品名称	种源地	北纬	东经	海拔/m	年降雨量/mm	最高温度/℃	最低温度/℃
1	鲁甸高杆	丽江市玉龙县	26°49'24''	100°03'41''	2490	955.80	19.39	7.85
2	华坪高杆	丽江市华坪县	26°43'17''	101°27'29''	2570	1040.33	27.32	13.88
3	永平矮杆	大理州永平县	25°29'21''	99°37'26''	2290	962.64	23.34	10.09
4	马龙高杆	曲靖市马龙区	25°33'20''	103°29'50''	2140	975.35	19.71	9.61
5	曲靖高杆	曲靖市麒麟区	25°15'31''	103°47'22''	2410	961.72	20.89	10.21
6	师宗矮杆	曲靖市师宗县	24°45'03''	103°59'24''	1890	1174.34	20.02	10.05
7	宣威矮杆	曲靖市师宗县	24°45'03''	103°59'24''	1890	1174.34	20.02	10.05
8	罗平高杆	曲靖市师宗县	24°45'03''	103°59'24''	1890	1174.34	20.02	10.05
9	罗平矮杆	曲靖市师宗县	24°45'03''	103°59'24''	1890	1174.34	20.02	10.05
10	泸西矮杆	曲靖市师宗县	24°33'32''	103°47'44''	1810	910.44	21.64	10.71

产地	净光合速率/[μmol/(m²·s)]	气孔导度/[μmol/(m²·s)]	蒸腾速率/[mmolH₂O/(m²·s)]	水分利用效率/(μmolCO₂/mmolH₂O)	Ci/Ca
丽江	3.27±0.60b	0.04±0.01ab	1.78±0.84b	2.12±1.07b	0.56±0.14a
华坪	6.97±0.49a	0.10±0.03d	5.72±1.38a	1.24±0.21b	0.64±0.05a
永平	7.32±0.27a	0.04±0.00a	1.52±0.19c	4.88±0.63a	0.21±0.05b
马龙	1.97±1.00c	0.02±0.00c	1.05±0.18b	1.81±0.69b	0.62±0.10a
曲靖	1.49±0.60c	0.02±0.00bc	1.10±0.19b	1.32±0.42b	0.69±0.08a
师宗	1.38±0.06c	0.02±0.00c	0.86±0.08b	1.63±0.22b	0.67±0.03a

产地	净光合速率/[μmol/(m²·s)]	气孔导度/[μmol/(m²·s)]	蒸腾速率/[mmolH₂O/(m²·s)]	水分利用效率/(μmolCO₂/mmolH₂O)	Ci/Ca
丽江	3.27±0.60b	0.04±0.01ab	1.78±0.84b	2.12±1.07b	0.56±0.14a
华坪	6.97±0.49a	0.10±0.03d	5.72±1.38a	1.24±0.21b	0.64±0.05a
永平	7.32±0.27a	0.04±0.00a	1.52±0.19c	4.88±0.63a	0.21±0.05b
马龙	1.97±1.00c	0.02±0.00c	1.05±0.18b	1.81±0.69b	0.62±0.10a
曲靖	1.49±0.60c	0.02±0.00bc	1.10±0.19b	1.32±0.42b	0.69±0.08a
师宗	1.38±0.06c	0.02±0.00c	0.86±0.08b	1.63±0.22b	0.67±0.03a

种源	净光合速率/[μmol/(m²·s)]	气孔导度/[μmol/(m²·s)]	蒸腾速率/[mmolH₂O/(m²·s)]	水分利用效率/(μmolCO₂/mmolH₂O)	Ci/Ca
师宗矮杆	4.41±2.51a	0.05±0.00a	2.17±0.12a	2.06±1.26a	0.55±0.24a
宣威矮杆	2.88±0.81a	0.04±0.02a	1.51±0.58b	1.97±0.28a	0.64±0.05a
罗平高杆	1.38±0.06b	0.02±0.00b	0.86±0.08c	1.63±0.22a	0.67±0.03a
罗平矮杆	2.22±0.68a	0.03±0.01ab	1.33±0.44b	1.69±0.19a	0.63±0.03a
泸西矮杆	2.43±0.56a	0.04±0.00ab	1.66±0.26ab	1.45±0.11a	0.67±0.03a