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中国农学通报 ›› 2022, Vol. 38 ›› Issue (36): 112-119.doi: 10.11924/j.issn.1000-6850.casb2022-0069

• 生物科学 • 上一篇    下一篇

基于SSR标记的芋头种质资源遗传多样性分析及抗疫病鉴定

郭巨先1(), 吴廷全2, 李沐蓉3, 唐康3, 姚春鹏1, 李桂花1, 罗文龙1, 王瑞2()   

  1. 1广东省农业科学院蔬菜研究所/广东省蔬菜新技术研究重点实验室,广州 510640
    2广东省农业科学院设施农业研究所,广州 510640
    3华南农业大学,广州 510642
  • 收稿日期:2022-02-11 修回日期:2022-05-02 出版日期:2022-12-25 发布日期:2023-01-17
  • 作者简介:

    郭巨先,女,1970年出生,湖南桃源人,研究员,硕士,主要从事特色蔬菜资源评价和利用研究。Tel:020-38469456,E-mail:

  • 基金资助:
    广东省科技计划项目“粤港澳大湾区国际科技创新中心建设”(2021A0505050004); 广东省农村科技特派员项目“特色蔬菜新品种引进与绿色栽培技术示范推广”(KTP20210007); 广东省科技计划项目“华南抗疫病蔬菜资源的收集、筛选、鉴评及应用技术开发”(2019B030316015); 广东省自然科学基金项目“CsPUB54与pmRXLR1互作负向调控黄瓜对疫病抗性的分子机理研究”(2019A1515011310)

Taro Germplasm Resources: Genetic Diversity Analysis and Resistance Identification to Blight Based on SSR Markers

GUO Juxian1(), WU Tingquan2, LI Murong3, TANG Kang3, YAO Chunpeng1, LI Guihua1, LUO Wenlong1, WANG Rui2()   

  1. 1Vegetable Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory for New Technology Research of Vegetables, Guangzhou 510640
    2Institute of Facility Agriculture, Guangdong Academy of Agricultural Sciences, Guangzhou 510640
    3South China Agricultural University, Guangzhou 510642
  • Received:2022-02-11 Revised:2022-05-02 Online:2022-12-25 Published:2023-01-17

摘要:

为了了解不同来源芋头种质资源的遗传多样性及其对疫病的抗性,本研究从100对SSR引物中筛选出21对多态丰富的SSR引物,并将其用于109份芋头资源的遗传多样性分析,同时采用人工接种芋头疫霉菌的方法对这些资源进性行了抗性评价。结果表明,21对SSR引物在109份芋头资源中共扩增出71个等位基因,变幅在2~4个,平均有效等位基因数3.38;观测杂合度(Ho)变化范围为0.0481~0.8900,平均为0.3483;预期杂合度(He)的变化范围为0.0841~0.6709,平均为0.3794;种群平均Shannon遗传多样性指数为0.6733;遗传距离在0~1之间,平均遗传距离为0.6014。在分支距离为0.49处,可将供试芋头资源分为3个类群。60%以上的抗性资源集中于I和II类群。本研究可为筛选、鉴定优良芋头种质资源及遗传育种工作提供科学依据。

关键词: 芋, 种质资源, SSR标记, 遗传多样性, 抗病评价

Abstract:

In order to investigate the genetic diversity of taro germplasm resources from different sources and detect their resistance to blight, 21 pairs of SSR marker primers with high polymorphism were screened out from 100 pairs of SSR marker primers, and were used to analyze the genetic diversity of taro resources. Meanwhile, the resistance of 109 germplasm resources was evaluated by inoculating taro Phytophthora artificially. Results showed that a total of 71 polymorphic alleles were detected and the number of alleles per locus ranged from 2 to 4 with an average of 3.38 alleles. The observed heterozygosity (Ho) ranged from 0.0481 to 0.8900 with an average of 0.3483. The expected heterozygosity (He) ranged from 0.0841 to 0.6709 with an average of 0.3794. The average Shannon genetic diversity index (I) was 0.6733, the genetic distance was between 0 and 1, and the average genetic distance was 0.6014. The taro germplasm resources were divided into three groups at the genetic distance of 0.49. More than 60% of resistant germplasm resources belonged to I and II groups. This study could provide a scientific basis for screening and identification of excellent taro germplasm resources and genetic breeding.

Key words: taro, germplasm resources, SSR marker, genetic diversity, resistance identification