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中国农学通报

中国农学通报 ›› 2023, Vol. 39 ›› Issue (13): 64-69.doi: 10.11924/j.issn.1000-6850.casb2022-0435

• 林学·园艺·园林 • 上一篇    下一篇

LED光质对2种石斛兰组培苗增殖、生根及生长的影响

吴瑶1,2(), 王再花1, 李杰1, 刘海林1, 段俊3, 周荣2, 叶广英1()   

  1. 1 广东省农业科学院环境园艺研究所/广东省园林花卉种质创新综合利用重点实验室，广州 510640
    2 佛山科学技术学院食品科学与工程学院，广东佛山 510000
    3 中国科学院华南植物园广东省应用植物学重点实验室/华南农业植物分子分析与遗传改良重点实验室，广州 510650
  • 收稿日期:2022-05-31 修回日期:2022-12-15 出版日期:2023-05-05 发布日期:2023-04-27
  • 通讯作者: 叶广英，男，1988年出生，山东聊城人，助理研究员，博士，主要从事康养花卉及南药资源开发研究。通信地址：510640 广东省广州市天河区金颖东一街1号 广东省农业科学院环境园艺所，E-mail：yeggyy20062009@163.com。
  • 作者简介:

    吴瑶，女，1997年出生，四川宜宾人，在读硕士，研究方向为食药用植物组培及栽培研究。通信地址：510640 广东省广州市天河区金颖东一街1号 广东省农业科学院环境园艺所，E-mail：。

  • 基金资助:
    广州市科技计划项目“纤维素酶处理提高大苞鞘石斛多糖抗氧化活性机理的研究”(202102021090); 广东省农业科学院协同创新中心项目“亚热带特产资源品质评价与精准提质增效加工技术研究与应用”(XT202229); 国家重点研发计划项目“石斛兰轻简高效栽培技术研发与集成示范”(2020YFD1000400); 广东省农业科技发展及资源环境保护管理项目“以农产品为单元的广东省现代农业产业技术体系创新团队建设项目（南药（化橘红））”(2022KJ148)

Influence of LED Light Quality on Proliferation, Rooting and Growth of Tissue Culture Plantets of Two Species of Dendrobium

WU Yao1,2(), WANG Zaihua1, LI Jie1, LIU Hailin1, DUAN Jun3, ZHOU Rong2, YE Guangying1()   

  1. 1 Environmental Horticulture Research Institute, Guangdong Academy of Agricultural Sciences/ Guangdong Provincial Key Lab of Ornamental Plant Germplasm Innovation and Utilization, Guangzhou 510640
    2 School of Food Science and Engineering, Foshan University of Science and Technology, Foshan, Guangdong 510000
    3 Guangdong Provincial Key Laboratory of Applied Botany/ Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement,South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650
  • Received:2022-05-31 Revised:2022-12-15 Online:2023-05-05 Published:2023-04-27

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摘要：

为提高石斛兰的组培效率，以‘甜心’石斛和‘红粉天宫’兜唇石斛为研究对象，研究白光（W，可见全光谱400~700 nm）、红蓝绿组合光(R3B6G0、R3B6G1、R3B6G3、R6B3G0、R6B3G1、R6B3G3、R3B3G3)共8种LED光质对石斛兰增殖与生根的影响，并系统比较增殖过程中2种石斛兰增殖系数、鲜重、干重和干物质含量的差异，以及生根过程中根数、平均根长、茎粗、新芽数、株高、叶数的差异。结果表明，‘甜心’石斛在R6B3G1的光质下增殖效果最好，培养45 d增殖系数达到2.55个/株，鲜重、干重和干物质含量分别为0.54 g/株、0.053 g/株和9.8%。其次为R6B3G0处理，而对照W和R3B6G3效果较差。生根方面，‘甜心’石斛则在R6B3G3组合光质处理下效果最佳，根数和平均根长分别为11.36根/株和2.64 cm，其次为R6B3G1，而对照W和R3B3G3效果最差。‘红粉天宫’兜唇石斛在R6B3G3光质组合增殖效果最好，增殖系数达到1.19个/株，鲜重、干重和干物质含量分别为0.718 g/株、0.0548 g/株和7.91%，其次为对照W，而R6B3G0效果较差。生根方面，‘红粉天宫’兜唇石斛在R6B3G1组合光质处理下效果最好，其次为R6B3G3，而R3B6G3和对照W效果较差。可见，在R6B3红蓝光质下适当补充一定绿光，有利于2种石斛兰增殖和生根壮苗。

关键词: LED, 光质, ‘甜心’石斛, ‘红粉天宫’兜唇石斛, 组织培养

Abstract:

This study compared the effects of white light (W), and red-blue-green combination light (R3B6G0, R3B6G1, R3B6G3, R6B3G0, R6B3G1, R6B3G3, and R3B3G3) on the proliferation and rooting status of Dendrobium Lucky Girls ‘Sweet Heart’ and Dendrobium aphyllum ‘Hongfentiangong’, to improve the production efficiency of their tissue culture. At this developmental stage, the differences of most morphometric parameters were compared, such as proliferation coefficient, fresh weight, dry weight and dry matter content, root number, average root length, stem diameter, new bud number, plant height and leaf number. Under the treatment of R6B3G1 for 45 days, D. Lucky Girls ‘Sweet Heart’ exhibited the best proliferation effects, with a proliferation coefficient of 2.55 per plant, and its fresh weight, dry weight and dry matter content were 0.54 g/plant, 0.053 g/plant and 9.8%, respectively. The effects of R6B3G0 treatment came as the second, while W and R3B6G3 treatment had poor effects. The root number and average root length of D. Lucky Girls ‘Sweet Heart’ were the best, reaching 11.36 per plant and 2.64 cm, under the treatment of R6B3G3, followed by those under R6B3G1 treatment, but the effects of W and R3B3G3 treatment were the worst. D. aphyllum ‘Hongfentiangong’ had the best proliferation effect under R6B3G3 treatment, with the proliferation coefficient reaching 1.19 per plant and its fresh weight, dry weight and dry matter content were 0.718 g/plant, 0.0548 g/plant and 7.91%, respectively; the effects under W were inferior to those under R6B3G3 treatment, and the worst effects were found under R6B3G0 treatment. In terms of rooting, the effect of R6B3G1 treatment was the best for D. aphyllum ‘Hongfentiangong’, followed by R6B3G3, while the effects of R3B6G3 and W were poor. Therefore, the combined application of red, blue and green light has great potential in terms of the proliferation and rooting for the two Dendrobium species.

Key words: LED, light quality, Dendrobium Lucky Girl ‘Sweet Heart’, Dendrobium aphyllum ‘Hongfentiangong’, tissue culture