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中国农学通报

中国农学通报 ›› 2023, Vol. 39 ›› Issue (16): 118-123.doi: 10.11924/j.issn.1000-6850.casb2022-0450

• 畜牧·动物医学·蚕·蜂 • 上一篇    下一篇

牛支原体诊断技术的研究进展

汪梦竹1,2(), 蒲飞洋1,2, 赵泽阳1,2, 冯茜莉1,2, 王慧慧1,2, 李易聪1,2, 李倬1,2, 赵永清1,2()   

  1. 1 西北民族大学生物医学研究中心，兰州 730030
    2 西北民族大学生命科学与工程学院，兰州 730010
  • 收稿日期:2022-06-06 修回日期:2022-10-09 出版日期:2023-06-05 发布日期:2023-05-22
  • 通讯作者: 赵永清，男，1979年出生，甘肃天水人，研究方向：天然小分子肽对动物病毒性疾病的作用研究。通信地址：730124 甘肃省兰州市榆中县夏官营镇三角街 西北民族大学，E-mail：450883800@qq.com。
  • 作者简介:

    汪梦竹，女，1997年出生，硕士，研究方向：病原体遗传进化。通信地址：730030 甘肃省兰州市西北新村1号 生物医学研究中心，E-mail：。

  • 基金资助:
    甘肃省自然科学基金项目“蛙类皮肤中抗菌肽的分离鉴定及活性研究”(21JR1RA221)

Research Progress in Diagnostic Techniques of Mycoplasma bovis

WANG Mengzhu1,2(), PU Feiyang1,2, ZHAO Zeyang1,2, FENG Xili1,2, WANG Huihui1,2, LI Yicong1,2, LI Zhuo1,2, ZHAO Yongqing1,2()   

  1. 1 Biomedical Research Center, Northwest Minzu University, Lanzhou 730030
    2 College of Life Science and Engineering, Northwest Minzu University, Lanzhou 730010
  • Received:2022-06-06 Revised:2022-10-09 Online:2023-06-05 Published:2023-05-22

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摘要：

支原体作为造成牛感染的重要病原体已在全球范围内广泛传播，可引发牛的一系列重大疾病。支原体传染性高且难以根治，因此需要快速和准确的诊断方法来预防和控制疾病的爆发。本研究简述了牛支原体诊断方法的发展和应用。传统的支原体的鉴定和诊断以分离培养为主。近年来，使用聚合酶链式反应从样本中检测支原体的技术方法日益成熟，与传统的诊断方法相比，聚合酶链式反应的诊断更高效，特异性和敏感性更强。目前有病原分离鉴定、分子生物学鉴定、血清学鉴定等方式可用于支原体分离株的分型，便于在疾病暴发调查中确定基因特征。通过间接ELISA进行的血清学诊断，可以检测血清和牛奶中的抗支原体抗体，且已在动物样本和BTM样本上得到应用。这些检测方法的综合应用，将有助于生产实践中病原体的精确检测及对牛群疾病发生状况的科学评估。

关键词: 牛支原体, ELISA, 乳腺炎, PCR, 临床诊断

Abstract:

Mycoplasma, as an important pathogen causing cattle infection, has been spread worldwide and can cause a series of serious diseases in cattle. Mycoplasma is highly contagious and difficult to eradicate, so rapid and accurate diagnostic methods are needed to prevent and control disease outbreaks. This article reviewed the development and application of different diagnostic methods for mycoplasma. The traditional identification and diagnosis of mycoplasma are mainly in isolation and culture. Recently, techniques for mycoplasma detection from samples using polymerase chain reaction (PCR) have increasingly matured. Compared with traditional diagnostic methods, PCR is more efficient, specific and sensitive. At present, pathogen isolation and identification, molecular biology identification, serological identification and other ways can be used for mycoplasma isolates typing, and easy to determine the gene characteristics in the disease outbreak investigation. Serological diagnosis by indirect ELISA can detect anti-mycoplasma antibodies in serum and milk, and has been demonstrated on single animal samples and BTM samples. The comprehensive application of these detection methods will contribute to the accurate detection of pathogens in production practice and the scientific assessment of the disease occurrence status of cattle herds.

Key words: Mycoplasma bovis, ELISA, mastitis, PCR, clinical diagnosis