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中国农学通报

中国农学通报 ›› 2023, Vol. 39 ›› Issue (28): 119-125.doi: 10.11924/j.issn.1000-6850.casb2022-0786

• 食品·营养·检测·安全 • 上一篇    下一篇

梨果皮色素含量的测定方法研究

吴悦菊(), 张晓杰, 贾卓隆, 周伊鑫, 黄平, 王晓明, 彭建营()   

  1. 河北农业大学园艺学院/河北省梨工程技术研究中心，河北保定 071000
  • 收稿日期:2022-09-13 修回日期:2023-04-06 出版日期:2023-10-05 发布日期:2023-09-25
  • 通讯作者: 彭建营，男，1965年出生，河北保定人，教授，主要从事果树种质资源、遗传育种和分子生物学研究。E-mail：pengzhuwen@sina.com。
  • 作者简介:

    吴悦菊，女，1997年出生，河北廊坊人，硕士研究生，研究方向：果树栽培生理及遗传育种。通信地址：071000 河北省保定市莲池区河北农业大学西校区，E-mail：。

  • 基金资助:
    河北省重点研发计划项目“梨优质丰产耐贮运新品种的选育”(20326337D); 河北省重点研发计划项目“梨苹果现代种业科技创新团队”(21326308D)

Study on Determination Method of Pigment Content of Pear Peel

WU Yueju(), ZHANG Xiaojie, JIA Zhuolong, ZHOU Yixin, HUANG Ping, WANG Xiaoming, PENG Jianying()   

  1. College of Horticulture, Hebei Agricultural University/ Pear Engineering and Technology Center of Hebei Province, Baoding, Hebei 071000
  • Received:2022-09-13 Revised:2023-04-06 Published-:2023-10-05 Online:2023-09-25

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摘要：

为找到适用于不同梨果皮颜色色素含量的测定方法，采用2种测定方法对‘新梨7号’‘秋月’‘鸭梨’‘玉露香’‘香红梨’5个不同果皮颜色的梨品种进行叶绿素和总类胡萝卜素含量的测定。结果表明，对于‘新梨7号’和‘玉露香’，80%丙酮浸提法和95%乙醇浸提法测得的总叶绿素含量、叶绿素a含量、叶绿素b含量和总类胡萝卜素含量均差异不显著。对于‘鸭梨’‘香红梨’‘秋月’，80%丙酮浸提法和95%乙醇浸提法测得的总类胡萝卜素含量差异不显著，但80%丙酮浸提法测得的总叶绿素含量、叶绿素a含量和叶绿素b含量显著高于95%乙醇浸提法测得的含量。80%丙酮浸提法适用于测定所有品种梨果皮的叶绿素含量和总类胡萝卜素含量。测定果皮颜色为绿色和绿色为底色有红晕的梨果皮叶绿素和总类胡萝卜素时，可优先选择95%乙醇浸提法。若只需测定总类胡萝卜素含量，所有果皮颜色的梨均可选择95%乙醇浸提法。

关键词: 梨, 果皮, 叶绿素, 类胡萝卜素, 测定方法

Abstract:

The paper aims to find a method suitable for determining the color pigment content of different pear peels. The chlorophyll content and total carotenoid content were determined for five pear varieties with different peel colors, namely ‘Xinli No.7’, ‘Akizuki’, ‘Yali’, ‘Yuluxiang’ and ‘Xianghongli’ by two methods. The results showed that for ‘Xinli No.7’ and ‘Yuluxiang’, the total chlorophyll content, chlorophyll a content, chlorophyll b content and total carotenoid content measured by the 80% acetone extraction method and the 95% ethanol extraction method weren’t significantly different. For ‘Yali’, ‘Xianghongli’ and ‘Akizuki’, the total carotenoid content measured by the 80% acetone extraction method and the 95% ethanol extraction method wasn’t significantly different, but the total chlorophyll content, chlorophyll a content and chlorophyll b content measured by the 80% acetone extraction method were significantly higher than the content measured by the 95% ethanol extraction method. The chlorophyll content and total carotenoid content of pear peels of all varieties can be determined by 80% acetone extraction. When determining the chlorophyll and total carotenoids of pear peel with green color and green as the background color with red halo, the 95% ethanol extraction method can be preferred. If the total carotenoid content is only required, all peel colors can be extracted with 95% ethanol.

Key words: pear, pericarp, chlorophyll, carotenoids, test methods