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中国农学通报

中国农学通报 ›› 2023, Vol. 39 ›› Issue (30): 114-122.doi: 10.11924/j.issn.1000-6850.casb2022-0886

• 生物科学 • 上一篇    下一篇

江西铅山红芽芋芋病毒种类lncRNA测序鉴定及其序列分析

尹明华1,2,3,4(), 江玲1, 姚慧婷1, 宋粤涛1, 王梦晴1, 王沛棋1, 张艺欣1,2,3,4()   

  1. 1 上饶师范学院生命科学学院，江西上饶 334001
    2 上饶农业技术创新研究院，江西上饶 334001
    3 上饶市药食同源植物资源保护与利用重点实验室，江西上饶 334001
    4 上饶市薯芋类作物种质保存与利用重点实验室，江西上饶 334001
  • 收稿日期:2022-11-23 修回日期:2023-03-15 出版日期:2023-10-25 发布日期:2023-10-19
  • 通讯作者: 张艺欣，女，1991年出生，江西上饶人，讲师，研究生，硕士，主要从事植物组织培养的研究。通信地址：334001 江西省上饶市信州区志敏大道401号 上饶师范学院生命科学学院，Tel：0793-8153721，E-mail：474303981@qq.com。
  • 基金资助:
    国家自然科学基金资助项目“江西铅山红芽芋超低温疗法脱毒抑制细胞凋亡抗早衰的机理研究”(32060092); “江西铅山红芽芋“超低温疗法脱毒”响应机制的研究”(31960079); 江西省教育厅科学技术研究项目“江西铅山红芽芋WUSCHEL-related homeobox、CLAVATA和AGAMOUS基因cDNA全长克隆、序列信息和功能分析”(GJJ211729); 上饶市科技局平台载体建设项目“上饶市薯芋类作物种质保存与利用重点实验室”(2020I001); “上饶市药食同源植物资源保护与利用重点实验室”(2019I017)

LncRNA Sequencing Identification of Virus Species and the Sequence Analysis of Jiangxi Yanshan Red Bud Taro

YIN Minghua1,2,3,4(), JIANG Ling1, YAO Huiting1, SONG Yuetao1, WANG Mengqing1, WANG Peiqi1, ZHANG Yixin1,2,3,4()   

  1. 1 College of Life Sciences, Shangrao Normal University, Shangrao, Jiangxi 334001
    2 Shangrao Agricultural Technology Innovation Research Institute, Shangrao, Jiangxi 334001
    3 Key Laboratory of Protection and Utilization of Medicinal and Edible Plant Resources in Shangrao City, Shangrao, Jiangxi 334001
    4 Shangrao Key Laboratory of Germplasm Conservation and Utilization of Potato and Taro Crops, Shangrao, Jiangxi 334001
  • Received:2022-11-23 Revised:2023-03-15 Published-:2023-10-25 Online:2023-10-19

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摘要：

探究江西铅山红芽芋的主要病毒种类及其序列信息，为其脱毒苗培育提供依据。采用lncRNA测序技术确定江西铅山红芽芋的病毒种类，并利用RT-PCR技术、生物信息学分析软件和qRT-PCR技术进行病毒验证、序列分析和组织表达分析。江西铅山红芽芋检测到芋花叶病毒（DsMV）和花椰菜花叶病毒（CaMV）两种病毒；DsMV和CaMV基因cDNA总长度分别为1780 bp和5412 bp，分别编码592和1803个氨基酸；DsMV和CaMV多聚蛋白二级结构均由α螺旋、β-片层、无规则卷曲构成，其三级结构均为单体蛋白；DsMV和CaMV在江西铅山红芽芋根、茎、叶中均有侵染，但均在叶中表达量最高。明确了侵染江西铅山红芽芋的主要病毒种类，并首次在江西铅山红芽芋上检测到CaMV。

关键词: 江西铅山红芽芋, 病毒种类鉴定, lncRNA测序, 芋花叶病毒, 花椰菜花叶病毒, 序列分析

Abstract:

The aims were to explore the main virus types and sequence information of Jiangxi Yanshan red bud taro, and provide a basis for its virus-free plantlet cultivation. LncRNA sequencing technology was used to determine the virus species of Jiangxi Yanshan red bud taro, and RT-PCR technology, bioinformatics analysis software, and qRT-PCR technology were used for virus validation, sequence analysis, and tissue expression analysis. Two viruses, taro mosaic virus (DsMV) and cauliflower mosaic virus (CaMV), were detected in red bud taro from Yanshan, Jiangxi. The total cDNA lengths of DsMV and CaMV genes were 1780 bp and 5412 bp, respectively, encoding 592 and 1803 amino acids. The protein secondary structure of DsMV and CaMV poly protein was composed of alpha helix, extended strand and random coil, and its tertiary structure was composed of monomeric proteins. DsMV and CaMV were both infected in the roots, stems, and leaves of Jiangxi Yanshan red bud taro, but both had the highest expression levels in the leaves. The main types of viruses infecting Jiangxi Yanshan red bud taro had been identified, and CaMV had been detected for the first time in Jiangxi Yanshan red bud taro.

Key words: Jiangxi Yanshan red bud taro, identification of virus species, lncRNA sequencing, DsMV, CaMV, sequence analysis