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中国农学通报

中国农学通报 ›› 2023, Vol. 39 ›› Issue (33): 140-146.doi: 10.11924/j.issn.1000-6850.casb2022-0934

• 食品·营养·检测·安全 • 上一篇    下一篇

高效液相色谱法测定黄芩中12种黄酮类成分的含量

李轲(), 田玉洁, 田雨晴, 李美嬉, 郝敬虹, 杨柳()   

  1. 农业应用新技术北京市重点实验室/植物生产国家级实验教学示范中心/北京农学院，北京 102206
  • 收稿日期:2022-11-23 修回日期:2023-03-15 出版日期:2023-11-25 发布日期:2023-11-22
  • 通讯作者: 杨柳，女，1967年出生，北京人，高级实验师，硕士，研究方向：分析化学和色谱仪器分析。通信地址：102206 北京市昌平区回龙观镇北农路7号 北京农学院科技综合楼A503，Tel：010-80799470，E-mail：yangliu600@sina.com。
  • 作者简介:

    李轲，女，1997年出生，河北石家庄人，硕士研究生，研究方向：植物资源利用与分析。通信地址：102206 北京市昌平区回龙观镇北农路7号 北京农学院，Tel：18101351512，E-mail：。

  • 基金资助:
    北京市种质创制和品种选育联合攻关项目(G20220628003)

Determination of Twelve Flavonoid Components in Scutellaria baicalensis by HPLC

LI Ke(), TIAN Yujie, TIAN Yuqing, LI Meixi, HAO Jinghong, YANG Liu()   

  1. Beijing Key Laboratory of New Technology in Agricultural Application/National Demonstration Center for Experimental Plant Production Education/Beijing University of Agriculture, Beijing 102206
  • Received:2022-11-23 Revised:2023-03-15 Published-:2023-11-25 Online:2023-11-22

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摘要：

旨在建立一种测定黄芩中12种黄酮类成分的方法。采用HPLC法，色谱柱为Agilent ZORBAX SB-C18 (4.6 mm×150 mm，5 μm)；检测波长为280 nm；流动相为乙腈-0.1%磷酸水溶液，流速为1.0 m L/min，进样量为10 μL。利用建立的提取条件和色谱条件对黄芩进行定性定量分析。测定黄芩中12种黄酮类物质在质量浓度范围内线性关系良好(r>0.98)，平均加样回收率野黄芩苷、芹菜素-7-O-β-D-吡喃葡萄糖苷、红花素、黄芩苷、木犀草素、白杨素-7-O-葡萄糖醛酸苷、汉黄芩苷、芹菜素、黄芩素、汉黄芩素、白杨素、千层纸素A分别为99.7214%、92.7039%、103.9755%、100.5837%、92.4819%、93.2465%、99.6387%、104.3866%、95.0767%、95.3245%、92.2161%、95.3995%(RSD<5)。本研究建立了一种测定黄芩中12种黄酮类成分的含量方法，此方法操作简便，重复性好，准确度高。

关键词: 黄酮化合物, 黄芩, 高效液相色谱法, 黄芩苷, 野黄芩苷

Abstract:

The aim was to establish a method for the determination of twelve flavonoid components in Scutellaria baicalensis. The HPLC method was performed on an Agilent ZORBAX SB-C18 column (4.6 mm×150 mm, 5μm) with a detection wavelength of 280 nm and a mobile phase of acetonitrile-0.1% phosphoric acid aqueous solution at a flow rate of 1.0 mL/min and a sample volume of 10 μL. The established extraction and chromatographic conditions were used for the qualitative and quantitative analysis of Scutellaria baicalensis. The linearity of the twelve flavonoids in Scutellaria baicalensis was good in the mass concentration range (r>0.98), and the average spiked recoveries of scutellarin, apigenin-7-O-β-D-glucopyranoside, carthamidin, baicalin, luteolin, chrysin-7-O-glucuronide, wogonoside, apigenin, baicalein, wogonin，chrysin and oroxylin A were 99.7214%, 92.7039%, 103.9755%, 100.5837%, 92.4819%, 93.2465%, 99.6387%, 104.3866%, 95.0767%, 95.3245%, 92.2161%, 95.3995% (RSD<5). A method for the determination of twelve flavonoid components in Scutellaria baicalensis was established, which was easy to operate, reproducible and accurate.

Key words: flavonoids, Scutellaria baicalensis, high performance liquid chromatography, baicalin, wild baicalin