欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2016, Vol. 32 ›› Issue (34): 187-192.doi: 10.11924/j.issn.1000-6850.casb16080148

• 食品 营养 检测 安全 • 上一篇    下一篇

高效液相色谱法测定黄皮果实中的有机酸

朱 敏,何书强,罗石荣,麦贤家,陈业渊   

  1. (中国热带农业科学院热带作物品种资源研究所/国家热带果树品种改良中心/海南省热带果树工程技术研究中心,海南儋州 571737）
  • 收稿日期:2016-08-31 修回日期:2016-11-23 接受日期:2016-10-25 出版日期:2016-12-15 发布日期:2016-12-15
  • 通讯作者: 朱 敏
  • 基金资助:
    国家国际科技合作专项项目“中南美洲热带作物种质资源收集与合作研究”(2011dfb31690)。

Determination of Organic Acids in Wampee [Clausena lansium (Lour.) Skeels] Fruits by High Performance Liquid Chromatography (HPLC)

Zhu Min, He Shuqiang, Luo Shirong, Mai Xianjia, Chen Yeyuan   

  1. (Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/ National Cultivar Improvement Center of Tropical Fruit Tree/Hainan Tropical Fruit Engineering Technology Research Center, Danzhou Hainan 571737)
  • Received:2016-08-31 Revised:2016-11-23 Accepted:2016-10-25 Online:2016-12-15 Published:2016-12-15

PDF (PC)

382

摘要： 为深入了解黄皮果实中有机酸，采用水浴超声提取果实中的有机酸、高效液相色谱法测定的方法，对12份黄皮种质果实中有机酸的组分及含量进行研究。结果表明：在色谱柱Venusil XBP C18(T) (4.6 mm×250 mm，5 μm)、流动相为0.01 mol/L (NH4)2HPO4（H3PO4调pH至2.7）、流速1 mL/min、柱温27℃、检测波长210 nm的条件下，黄皮果实中柠檬酸、草酸、乳酸、苹果酸、抗坏血酸、酒石酸得到良好分离，且测定精确灵敏、回收率高、重复性好，标准曲线相关系数为0.9975~0.9996，相对标准偏差为1.02%~1.84%，回收率为95.60%~101.78%。黄皮果实中有机酸以柠檬酸、草酸和苹果酸为主，乳酸在少部分种质中有测出，不同种质果实中柠檬酸、草酸、苹果酸含量分别为0.780~8.090、0.773~4.205、0.478~ 3.212 mg/(g.FW)，分别占总酸的22%~65%、16%~40%、11%~20%。

关键词: 蜡样芽孢杆菌, 蜡样芽孢杆菌, 猕猴桃溃疡病, 丁香假单胞菌猕猴桃致病变种, 生物防治

Abstract: To study the organic acids in wampee fruits, the constituent and content of organic acid in 12 wampee germplasms were analyzed by ultrasonic-assisted water extraction and high performance liquid chromatography (HPLC). The results show that six organic acids can be successfully separated and all the calibration curves for these organic acids display a good linear relationship with correlation coefficients of 0.9975-0.9996, RSD is 1.02%-1.84% (n=5) and recovery rate is 95.60%-101.78%, on the condition that Venusil XBP C18(T) column (4.6 mm×250 mm, 5 μm) is used at 27℃, mobile phase is 0.01 mol/L (NH4)2HPO4 (pH 2.6) with flow rate of 1 mL/min and detection wavelength of 210 nm. The major parts of organic acids in wampee fruits are citric acid, oxalix acid and malic acid, lactic acid is detected only in a few samples; in different samples, the content of citric acid, oxalic acid and malic acid is 0.780-8.090 mg/(g.FW), 0.773-4.205 mg/(g.FW), 0.478-3.212 mg/(g.FW), respectively, and accounts for 22%-65%, 16%-40% and 11%-20% of the total acid.

中图分类号: