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中国农学通报

中国农学通报 ›› 2024, Vol. 40 ›› Issue (17): 49-55.doi: 10.11924/j.issn.1000-6850.casb2023-0525

• 资源·环境·生态·土壤·气象 • 上一篇    下一篇

砷对蚯蚓的滤纸接触毒性及硒的调节作用

杨莹1,2(), 王志敏1,2, 杜晶晶2, 周子涵2, 周升志2, 符晓雯2, 王瑞萍1, 岳士忠1()   

  1. 1 德州学院生物物理研究院/山东省生物物理重点实验室，山东德州 253023
    2 德州学院生命科学学院，山东德州 253023
  • 收稿日期:2023-07-14 修回日期:2023-11-28 出版日期:2024-06-15 发布日期:2024-06-11
  • 通讯作者:
    岳士忠，男，1988年出生，河南周口人，副教授，博士，研究方向：生态毒理学。E-mail：。
  • 作者简介:

    杨莹，女，2003年出生，山东临沂人，本科，研究方向：生物科学。通信地址：253023 山东省德州市德城区大学西路566号，E-mail：。

  • 基金资助:
    德州学院人才引进项目“硒抑制蚯蚓对砷的生物富集及其作用机理”(2019XJRC328); 山东省高等学校（省级）大学生创新创业训练计划项目“土壤镉污染对蚯蚓的生理胁迫及硒的缓解作用”(S202310448127); 山东省高等学校（校级）大学生创新创业训练计划项目“硒调解中金属砷诱导的蚯蚓氧化应激胁迫及其作用机理研究”(X202110448060)

The Contact Toxicity of Arsenic to Earthworms and Regulatory Effect of Selenium

YANG Ying1,2(), WANG Zhimin1,2, DU Jingjing2, ZHOU Zihan2, ZHOU Shengzhi2, FU Xiaowen2, WANG Ruiping1, YUE Shizhong1()   

  1. 1 Institute of Biophysics, Dezhou University/Shandong Key Laboratory of Biophysics, Dezhou, Shandong 253023
    2 College of Life Sciences, Dezhou University, Dezhou, Shandong 253023
  • Received:2023-07-14 Revised:2023-11-28 Published:2024-06-15 Online:2024-06-11

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摘要：

为探究重金属砷(As)对蚯蚓的滤纸接触急性毒性及硒(Se)的调节效果，将赤子爱胜蚓(Eisenia fetida)在不同Se (IV)浓度的基质（滤纸和土壤）中暴露适应后再进行As (V)的滤纸接触法急性毒性试验。结果表明：蚯蚓直接暴露于As染毒滤纸上的24、48 h的半致死浓度(LC50)分别为31.33、12.30 μg/cm2；蚯蚓在Se浓度为0.5 μg/cm2的湿润滤纸上适应6 h后，As对其的LC50分别为30.20 μg/cm2 (24 h)和15.85 μg/cm2 (48 h)；蚯蚓在Se浓度为6、12 mg/kg的土壤中适应一周后，As对其的LC50分别为34.67~35.48 μg/cm2 (24 h)和18.62~21.88 μg/cm2 (48 h)。蚯蚓经含Se滤纸和土壤适应后在48 h的LC50有所升高，说明Se能够在一定程度上提高蚯蚓短时间内对As的耐受能力。本研究为缓解土壤生物所受污染胁迫提供了新的研究思路与方法。

关键词: 砷, 硒, 赤子爱胜蚓, 接触毒性, 缓解

Abstract:

To investigate the acute filter paper contact toxicity of heavy metal arsenic (As) to earthworms and the regulatory effect of selenium (Se), Eisenia fetida was exposed to two media (filter paper and soil) with different concentrations of Se, and then the acute toxicity test of As was conducted by filter paper contact method. The results showed that the half lethal concentrations (LC50) on E. fetida directly exposed to As (V) contaminated filter paper for 24 and 48 h were 31.33 and 12.30 μg/cm2, respectively. After a 6 h period of adaptation on wet filter paper with Se concentration of 0.5 μg/cm2, the LC50 of As on E. fetida were 30.20 (24 h) and 15.85 μg/cm2 (48 h), respectively. After adapting to soil with Se concentrations of 6 and 12 mg/kg for one week, the LC50 of As to earthworms were 34.67-35.48 μg/cm2 (24 h) and 18.62-21.88 μg/cm2 (48 h), respectively. In conclusion, the pre-exposure of earthworms in Se-containing filter paper and soil both stimulated the improvement of LC50 values at 48 h, indicating that Se can improve the ability of earthworms to tolerate As stress during short exposure periods. This study provides new research ideas and methods for alleviating heavy metal pollution stress on soil biota.

Key words: arsenic, selenium, Eisenia fetida, exposure toxicity, alleviate