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中国农学通报 ›› 2025, Vol. 41 ›› Issue (11): 146-151.doi: 10.11924/j.issn.1000-6850.casb2023-0781

• 植物保护 • 上一篇    下一篇

葡萄孢属菌株HY-021对藜的侵染机制研究

朱海霞(), 李欢, 程亮   

  1. 青海大学农林科学院/农业部西宁作物有害生物科学观测实验站/青海省农业有害生物综合治理重点实验室,西宁 810016
  • 收稿日期:2024-08-06 修回日期:2024-10-25 出版日期:2025-04-15 发布日期:2025-04-11
  • 作者简介:

    朱海霞,女,1985年出生,副研究员,博士,研究方向:生物防治。通信地址:810016 中国青海省西宁市城北区宁大路253号,E-mail:

  • 基金资助:
    青海省科技国际合作专项(2023-HZ-808)

Study on Infection Mechanism of Botrytis spp. Strain HY-021 on Chenopodium album L.

ZHU Haixia(), LI Huan, CHENG Liang   

  1. Academy of Agriculture and Forestry Sciences of Qinghai University/Scientific Observing and Experimental Station of Crop Pest in Xining, Ministry of Agriculture and Rural Affairs /Key Laboratory of Agricultural Integrated Pest Management in Qinghai Province, Xining 810016
  • Received:2024-08-06 Revised:2024-10-25 Published:2025-04-15 Online:2025-04-11

摘要:

为了明确除草活性菌株HY-021对藜(Chenopodium album L.)的侵染作用机理,利用电镜扫描技术观察该菌株接种藜后对藜叶片的侵染过程,并使用生理指标测定法对接种后藜植株体内活性物质含量变化进行了测定。结果表明,HY-021菌株的菌丝通过气孔侵入叶片组织,并寄生繁殖产孢,逐渐破坏组织,同时叶片表面开始失绿并伴随着病斑的形成,最终导致植株不能进行光合作用而死亡。HY-021菌株接种于藜植株后体内MDA含量逐渐升高,SOD、CAT、POD的活性呈现出先上升后下降的趋势。表明该菌株对植株生理防御机制中的酶有多方面的影响,接种后引起植物广泛的代谢变化。可溶性蛋白质含量总体上高于对照,表明受到菌株侵染后,可溶性蛋白质含量增加,植物的抵御能力增强。

关键词: 除草菌株HY-021, 藜, 侵染过程, 生理机制, 除草活性

Abstract:

In order to clarify the mechanism of herbicidal activity of strain HY-021 on Chenopodium album L., electron microscope scanning technology was used to observe the process of infestation of C. album leaves by the strain after inoculation, and changes in the activity content of C. album plants after inoculation were determined using physiological index assay. The results showed that the mycelium of the HY-021 strain invaded leaf tissues through stomata, parasitized, proliferated and produced spores, which gradually destroyed the tissue. Concurrently, leaf surfaces began to show chlorosis and developed disease spots, ultimately leading to plant death due to an inability to perform photosynthesis. Following inoculation of C. album plants with the HY-021 strain, the content of MDA inside the plant bodies gradually increased, while the activities of enzymes such as SOD, CAT, and POD showed trend of increasing and then decreasing after inoculation. It showed that the strain had multiple effects on the plant physiological mechanisms and defense enzymes, and caused a wide range of metabolic changes in the plant after inoculation. The overall content of soluble proteins was higher than the control, indicating that soluble protein content increased following infection by the strain, which enhanced the plant's resistance capabilities.

Key words: herbicidal strain HY-021, Chenopodium album L., infection process, physiological mechanism, herbicidal activity