欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2025, Vol. 41 ›› Issue (18): 47-56.doi: 10.11924/j.issn.1000-6850.casb2024-0275

• 农学·农业基础科学 • 上一篇    下一篇

不同浓度PEG6000干旱胁迫处理的三七叶片代谢组分析

王晓静(), 孙宏伟()   

  1. 云南省文山州农业科学院，云南文山 663000
  • 收稿日期:2024-04-07 修回日期:2024-08-15 出版日期:2025-06-25 发布日期:2025-07-06
  • 通讯作者:
    孙宏伟，男，1982年出生，河南周口人，高级农艺师，博士，从事中药材病虫害研究。通信地址：663000 云南省文山州文山市新平街道泰康西路2号文山州农业科学院，Tel：0876-3037538，E-mail：。
  • 作者简介:

    王晓静，女，1988年出生，湖北荆州人，农艺师，硕士，主要从事中药材高效栽培及质量控制研究。通信地址：663000 云南省文山州文山市新平街道泰康西路2号文山州农业科学院，Tel：0876-3037538，E-mail：。

  • 基金资助:
    文山州科研发展专项资金项目“文山特色中药材根腐病拮抗细菌的分离鉴定及应用研究”(WS-KYFZXM23009)

Metabonomic Analysis of Panax notoginseng Leaves Under Drought Stress with Different Concentrations of PEG 6000

WANG Xiaojing(), SUN Hongwei()   

  1. Wenshan Academy of Agricultural Sciences, Wenshan, Yunnan 663000
  • Received:2024-04-07 Revised:2024-08-15 Published:2025-06-25 Online:2025-07-06

RichHTML

1

PDF (PC)

7

摘要：

为了解不同浓度PEG6000处理对三七叶片代谢产物及代谢通路的影响，以1年生三七为材料，采用自然干旱(CK)、2.5%PEG6000 (HE)、5%PEG6000 (HM)、7.5%PEG6000 (HK) 4种处理方式。用液相色谱-质谱(LC-MS)技术测定不同方式处理的三七叶片代谢产物。2.5%PEG6000干旱处理后三七叶片差异代谢物氨基酸类、甘油酯类、脂肪酰类、核苷酸类、内酯类、有机酸类、酮类和脂质类等化合物显著上调表达，并在甘油磷脂代谢、α-亚麻酸代谢、神经鞘脂质代谢、聚酮糖单元生物合成等通路上显著富集。5%PEG6000干旱处理后三七叶片差异代谢物生物碱类、氨基酸类、甘油磷脂类、苯类、脂质类和杂环类等化合物显著上调表达，并在亚油酸代谢、类胡萝卜素生物合成、核苷酸代谢、玉米素生物合成等通路上显著富集。7.5%PEG6000干旱处理后三七叶片差异代谢物黄酮类、甘油磷脂类、脂质类和杂环类等化合物显著上调表达，并在次生代谢产物的生物合成、卟啉代谢、核黄素代谢、β-丙氨酸代谢等通路上显著富集。不同浓度PEG6000处理后三七叶片的代谢产物存在一定差异，并通过不同的代谢途径来应答干旱胁迫，进而提高自身的抗旱能力。

关键词: 三七, PEG 6000, 干旱胁迫, 液相色谱-质谱, 代谢组, 代谢产物

Abstract:

In order to understand the effects of drought stress with different concentrations of PEG6000 on metabolites and metabolic pathway of Panax notoginseng leaves, one-year-old Panax notoginseng were treated by natural drought (CK), 2.5% PEG6000 (HE), 5% PEG6000 (HM), and 7.5% PEG6000 (HK). The metabolites of Panax notoginseng leaves treated in different methods were measured by using liquid chromatography-mass spectrometry (LC-MS) technology. The results showed that the metabolites of amino acids, glycerides, fatty acyl, nucleotides, lactones, organic acids, ketones and lipids were significantly up-regulated in expression under drought stress by 2.5% PEG 6000, and were significantly enriched in metabolic pathways such as glycerophosphate metabolism, α-linolenic acid metabolism, nerve sheath lipid metabolism and polyketide unit biosynthesis. After drought stress by 5% PEG6000, the metabolites of alkaloids, amino acids, glycerophosphates, benzenes, lipids and heterocyclic compounds were significantly up-regulated in expression, and were significantly enriched in metabolic pathways such as linoleic acid metabolism, carotenoid biosynthesis, nucleotide metabolism, and zeatin biosynthesis. After drought stress by 7.5% PEG6000, the metabolites of flavonoids, glycerophosphates, lipids and heterocyclic compounds were significantly up-expressed in expression, and were significantly enriched in metabolic pathways such as secondary metabolites biosynthesis, porphyrin metabolism, riboflavin metabolism and β-alanine metabolism. The metabolites of Panax notoginseng leaves existed some differences under drought stress with different concentrations of PEG6000, and they responded to drought stress through different metabolic pathways, thus to improve their ability of drought resistance.

Key words: Panax notoginseng, PEG6000, drought stress, liquid chromatography-mass spectrometry, metabolomic, metabolite