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中国农学通报 ›› 2025, Vol. 41 ›› Issue (1): 13-18.doi: 10.11924/j.issn.1000-6850.casb2024-0015

• 农学·农业基础科学 • 上一篇    下一篇

PEG模拟干旱胁迫下不同玉米品种萌芽期及苗期抗旱性鉴定

徐奕琳(), 徐东东, 张盼盼, 张逸康, 李鲜花()   

  1. 榆林学院生命科学学院,陕西榆林 719000
  • 收稿日期:2024-01-11 修回日期:2024-04-15 出版日期:2025-01-05 发布日期:2025-01-01
  • 通讯作者:
    李鲜花,女,1978年出生,山西阳泉人,副教授,硕士,主要从事作物遗传育种研究。通信地址:719000 榆林学院生命科学学院,Tel:0912-3891342,E-mail:
  • 作者简介:

    徐奕琳,女,1999年出生,贵州黔南人,在读硕士研究生,主要从事作物遗传育种研究。通信地址:719000 榆林学院生命科学学院,E-mail:

  • 基金资助:
    国家自然科学基金项目(31760209); 榆林市科技计划项目(CXY202190); 榆林学院2024年研究生创新基金项目(2024YLYCX17)

Drought Resistance Evaluation of Different Maize Varieties During Germination and Seedling Stage Under PEG Simulated Drought Stress

XU Yilin(), XU Dongdong, ZHANG Panpan, ZHANG Yikang, LI Xianhua()   

  1. College of Life Science, Yulin University, Yulin, Shaanxi 719000
  • Received:2024-01-11 Revised:2024-04-15 Published:2025-01-05 Online:2025-01-01

摘要:

为研究PEG模拟干旱胁迫下玉米萌发指标和生理指标变化,设置20% PEG-6000进行干旱处理,对玉米萌芽期发芽率、发芽势、萌发指数、胚根长及苗期叶绿素值(SPAD)、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量等指标进行测定,并采用隶属函数法对不同品种的抗旱性进行评价。结果显示,在干旱胁迫条件下,各玉米品种发芽率、发芽势、萌发指数、胚根长较对照均有所下降,叶绿素含量和SOD活性降低,MDA含量增加。利用隶属函数法评价玉米抗旱性,‘新农008’的隶属函数平均值大于0.7,属于强抗旱品种;‘中榆9号’、‘中榆1号’和‘沃玉3号’隶属函数平均值均小于0.4,属于弱抗旱品种。

关键词: PEG模拟干旱胁迫, 玉米, 萌芽期, 苗期, 生理指标, 抗旱性, 隶属函数法

Abstract:

In order to study the changes of germination index and physiological index of maize under PEG simulated drought stress, 20% PEG-6000 was set up for drought treatment. The germination rate, germination potential, germination index, radicle length, chlorophyll value (SPAD), superoxide dismutase (SOD) activity and malondialdehyde (MDA) content of maize at germination stage were measured, and the drought resistance of different varieties was evaluated by membership function method. The results showed that compared with CK, the germination rate, germination potential, germination index and radicle length of each maize variety decreased under drought stress, the chlorophyll value and SOD activity decreased, and the MDA content increased. The average value of the membership function of ‘Xinnong 008’ was greater than 0.7, which was a strong drought-resistant variety. The average value of membership function of ‘Zhongyu No.9’, ‘Zhongyu No.1’ and ‘Woyu No.3’ was less than 0.4, which belonged to weak drought-resistant varieties.

Key words: PEG simulated drought stress, corn, embryonic stage, seedling stage, physiological index, drought resistance, membership function method