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中国农学通报

中国农学通报 ›› 2024, Vol. 40 ›› Issue (35): 49-55.doi: 10.11924/j.issn.1000-6850.casb2024-0202

• 林学·园艺·园林 • 上一篇    下一篇

患根结线虫病三七的根际土壤细菌群落特征分析

彭翠仙(), 王灿, 李雪萍, 屈用函, 孙宏伟, 杨玉玲, 赵大伟, 陶永宏()   

  1. 云南省文山州农业科学院，文山云南 663000
  • 收稿日期:2024-03-19 修回日期:2024-07-22 出版日期:2024-12-15 发布日期:2024-12-12
  • 通讯作者:
    陶永宏，男，1982年出生，云南红河人，研究员，博士，研究方向：中药材种质资源遗传育种研究。通信地址：663000 云南省文山壮族苗族自治州文山市泰康西路2号，Tel：0876-3037538，E-mail：。
  • 作者简介:

    彭翠仙，女，1988年出生，云南曲靖人，农艺师，硕士，研究方向：中药资源收集及开发利用研究。通信地址：663000 云南省文山壮族苗族自治州文山市泰康西路2号，Tel：0876-3037538，E-mail：。

  • 基金资助:
    绿色高效三七种植技术提升与应用示范(文财农[2024]3号); 道地药材（三七、草果）林下生态高效栽培关键技术集成示范(202304BP090026); 文山州兴文英才项目(WS-KJLJ0009); 云南省科技特派队-麻栗坡县下金厂乡铁厂乡科技特派队(WS031020)

Analysis of Rhizosphere Bacterial Community Characteristics in the Disease Caused by Root-knot Nematodes on Panax notoginseng

PENG Cuixian(), WANG Can, LI Xueping, QU Yonghan, SUN Hongwei, YANG Yuling, ZHAO Dawei, TAO Yonghong()   

  1. Wenshan Academy of Agricultural Sciences, Wenshan, Yunnan 663000
  • Received:2024-03-19 Revised:2024-07-22 Published:2024-12-15 Online:2024-12-12

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摘要：

根结线虫病主要危害三七根部，同时也导致三七病原侵入引起复合侵染从而造成根的坏死与腐烂，影响三七产量。为了解健康三七与患根结线虫病的三七根际土壤中细菌群落多样性。通过收集健康三七根际土壤(HK)、根结线虫病三七根际土壤(GB)和空白对照(CK)，并利用16S rRNA基因对细菌群落进行多样性分析，结果显示健康三七皂苷含量(Rg1、Re、Rd)显著高于根结线虫病三七。在群落多样性分析中，健康三七根际细菌群落独有OTU个数为497个显著高于感病三七427个和CK 460个。多样性指数比较中(ace、chao1、Shannon)健康与根结线虫病三七根际细菌群落无显著差异，但均显著高于空白对照(CK)，PCoA分析3者细菌群落结构相似性较低(R2=0.544，P=0.008)，各处理土壤中优势细菌存在差异，CK中Bradyrhizobium、JG30a-KF-32、Saccharimonadales、Granulicella、WPS-2等菌属显著富集；健康三七中显著富集的是Chujaibacter、Bryobacter、AD3等；根结线虫病三七中Gemmatimonas、Rhodanobacte等显著富集。根结线虫侵染使三七皂苷含量下降，同时LEfSe分析指示健康三七根际中富集更多生防菌如Bryobacter等，这为三七致病菌的生物防治工作提供了新的思路。

关键词: 三七, 根结线虫, 细菌多样性, 16S rRNA, 皂苷含量, 生防菌

Abstract:

Root-knot nematodiasis mainly harms the roots of Panax notoginseng, meanwhile, its compound infection was caused by pathogen invasion of panax notoginseng, which leads to the necrosis and decay of root and ultimately made its yield was decreased. To understand the diversity of bacterial community in rhizosphere soil of Panax notoginseng in both healthy and it was invaded root-knot nematode disease conditions. We collected samples come from healthy soil (HK), infected soil (GB) and blank control (CK), and their bacterial communities were analyzed by using 16S rRNA sequencing. The results showed that the content of saponins (Rg1, Re, Rd) in healthy Panax notoginseng was significantly higher than that in affected by root-knot nematode. In the analysis of community diversity, the number of OTU was 497 in the rhizosphere bacterial community of healthy panax notoginseng, which was significantly higher than that of 427 in susceptible panax notoginseng and of 460 in CK. In the comparison of diversity index (ace, chao1, Shannon), although rhizosphere bacterial community had not significant difference in healthy and root-knot nematode, but it was significantly higher than that of blank control (CK). PCoA analysis showed that the similarity of bacterial community structure was low for three groups treatments (R2=0.544, P=0.008). However, the dominant bacteria existed differences in each treatment soil. Bradyrhizobium, JG30a-KF-32, Saccharimonadales, Granulicella and WPS-2 were significantly enriched in CK. Chujaibacter, Bryobacter, AD3 were significantly enriched in healthy Panax notoginseng. Gemmatimonas and Rhodanobacte were significantly enriched in Panax notoginseng was infected root-knot nematodiasis. The content of saponins was decreased by infecting root-knot nematodes. LEfSe analysis demonstrated that more biocontrol bacteria such as Bryobacter were enriched in the rhizosphere of healthy panax notoginseng, which provided help for the biological control of pathogenic bacteria of panax notoginseng.

Key words: Panax notoginseng, root-knot nematodes, bacterial diversity, 16S rRNA, saponin content, biocontrol bacteria