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中国农学通报 ›› 2023, Vol. 39 ›› Issue (25): 54-63.doi: 10.11924/j.issn.1000-6850.casb2022-0831

• 林学·园艺·园林 • 上一篇    下一篇

施用微生物菌剂对林下三七品质的影响

张澳1(), 李彪2, 何舒1, 熊冰杰1, 严星茹1, 黄佑国1, 王澍1, 施蕊1()   

  1. 1 西南山地森林资源保育与利用教育部重点实验室/昆明生态林业产业国际科技研发中心/西南林业大学园林与园艺学院,昆明 650224
    2 玉溪三农高原特色现代农业有限责任公司,云南澄江 652500
  • 收稿日期:2022-10-08 修回日期:2023-05-18 出版日期:2023-09-05 发布日期:2023-08-28
  • 通讯作者: 施蕊,女,1982年出生,云南昆明人,教授,博士,研究方向:天然药物化学。通信地址:650224 云南省昆明市盘龙区青云街道300号 西南林业大学,Tel:0871-63863380,E-mail:shirui@swfu.edu.cn
  • 作者简介:
    张澳,男,1999年出生,山东济宁人,在读硕士,研究方向:林下中药材生态种植。通信地址:650224 云南省昆明市盘龙区青云街道300号 西南林业大学,Tel:0871-63863380,E-mail:
  • 基金资助:
    国家自然科学基金“22GJ根际土壤微生态系统对林下三七药效形成的反馈机制研究”(32260720); 国家重点研发计划项目“林下中药材优质生产关键技术研究与集成示范”(2021YFD1000202); 国家现代农业产业技术体系(CARS-21-05B); 云南省重大科技专项“2021YK林下石斛、天麻有机种植关键技术及森林生态系统评估体系研究”(202102AE090042); 云南省级财政林业科技推广示范专项“澳洲坚果良种采橞圃营建及种植示范推广”(〔2020〕ts09号); 昆明市科技揭榜制项目“22KM林下三七产品开发和品牌打造”(2021JH002)

Effect of Microbial Fungicide Application on the Quality of Forest Panax notoginseng

ZHANG Ao1(), LI Biao2, HE Shu1, XIONG Bingjie1, YAN Xingru1, HUANG Youguo1, WANG Shu1, SHI Rui1()   

  1. 1 Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education/ International Ecological Foresty Research Center of Kunming/ College of Horticulture and Landscape Architecture, Southwest Forestry University, Kunming 650224
    2 Yuxi Sannong Plateau Characteristic Modern Agriculture Co., Ltd., Chengjiang, Yunnan 652500
  • Received:2022-10-08 Revised:2023-05-18 Online:2023-09-05 Published:2023-08-28

摘要:

为进一步提高云南省林下三七品质,采用液相色谱质谱(LC-MS)技术,同时结合多元统计分析,进行微生物菌剂林下三七与未施用菌剂林下三七根部糖醇类、维生素类和其他类差异代谢物筛选和KEGG注释。2组三七根中共检测到代谢物82种。差异代谢物26种,上调11种,包括糖醇类9种、维生素类2种;下调15种,包括糖醇类11种、维生素类2种、其他类2种。施用微生物菌剂的三七根中部分糖醇类、维生素代谢物增多,如D-苏糖醇、核酮糖-5-磷酸和维生素C(抗坏血酸)等,可见施用微生物菌剂对林下三七品质提升有一定促进作用。但菌剂施用也导致了三七中部分代谢物含量的降低,如阿魏酸钠、piscidionl A和D-氨基葡萄糖等。

关键词: 差异代谢物, 微生物菌剂, 糖醇类, 维生素, 林下三七

Abstract:

To further improve the quality of forest Panax notoginseng in Yunnan Province, liquid chromatography-mass spectrometry (LC-MS) technique combined with multivariate statistical analysis was used for the screening and KEGG annotation of sugar alcohols, vitamins and other classes of differential metabolites in the roots of forest P. notoginseng applied with microbial fungicides. A total of 82 metabolites were detected in the 2 groups of P. notoginseng roots. There were 26 different metabolites, 11 up-regulated, including 9 sugar alcohols and 2 vitamins; 15 down-regulated, including 11 sugar alcohols, 2 vitamins and 2 others. Some sugar alcohols and vitamin metabolites, such as D-threitol, ribulose-5-phosphate and vitamin C (ascorbic acid), were increased in P. notoginseng roots with microbial fungicide application, which showed that microbial fungicide application had some promotion effect on the quality improvement of P. notoginseng under the forest. However, microbial fungicide application also led to a decrease in the content of some metabolites in P. notoginseng, such as sodium ferulate, piscidionl A and D-glucosamine.

Key words: differential metabolites, microbial fungicides, sugar alcohols, vitamins, forest Panax notoginseng