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中国农学通报

中国农学通报 ›› 2025, Vol. 41 ›› Issue (19): 30-35.doi: 10.11924/j.issn.1000-6850.casb2024-0616

• 生物科学 • 上一篇    下一篇

卷丹百合LlAGPS1基因克隆与表达分析

张进忠1(), 孙嘉曼2, 孙明艳3, 黄绍力1()   

  1. 1 广州市农业农村科学院，广州 510335
    2 嘉应学院生命科学学院，广东梅州 514015
    3 广西农业科学院，南宁 530007
  • 收稿日期:2024-09-27 修回日期:2024-12-15 出版日期:2025-07-05 发布日期:2025-07-10
  • 通讯作者:
    黄绍力，男，1972年出生，广西贺州人，研究员，硕士，研究方向：作物遗传育种。通信地址：510335 广州市海珠区琶洲大道17-19号 广州市农业农村科学院，Tel：020-86392433，E-mail：。
  • 作者简介:

    张进忠，男，1979年出生，湖南澧县人，副研究员，博士，研究方向：作物遗传育种。通信地址：510335 广州市海珠区琶洲大道17-19号 广州市农业农村科学院，Tel：020-86392433，E-mail：。

  • 基金资助:
    广西农业科学院基本科研业务专项“百合组培鳞茎培育研究”(桂农科2019M26); 嘉应学院引进人才科研启动项目(2022RC97)

Cloning and Expression Analysis of LlAGPS1 Gene in Lilium lancifolium

ZHANG Jinzhong1(), SUN Jiaman2, SUN Mingyan3, HUANG Shaoli1()   

  1. 1 Guangzhou Academy of Agricultural and Rural Sciences, Guangzhou 510335
    2 School of Life Sciences, Jiaying University, Meizhou, Guangdong 514015
    3 Guangxi Academy of Agricultural Sciences, Nanning 530007
  • Received:2024-09-27 Revised:2024-12-15 Published:2025-07-05 Online:2025-07-10

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摘要：

腺苷二磷酸葡萄糖焦磷酸化酶(ADP-glucose pyrophosphorylase，AGPase)是植物淀粉合成途径的限速酶。克隆卷丹百合AGPase同源基因并分析其表达特征，有助于深入了解珠芽生长发育期的淀粉合成规律，为开发利用珠芽作为种子的价值或膳食功能提供依据。本研究运用同源克隆技术，从卷丹百合中成功克隆到一个腺苷二磷酸葡萄糖焦磷酸化酶小亚基编码基因(LlAGPS1)，并采用荧光定量PCR技术对LlAGPS1在叶片、珠芽组织部位的表达差异进行分析。研究表明，LlAGPS1基因编码蛋白具有PLN02241、GlgC保守结构域，属于cl33437超级家族蛋白；蛋白序列比对显示，其与其他作物AGPase小亚基序列具有较高同源性。qPCR分析表明，LlAGPS1基因主要在珠芽中表达，其表达量显著高于叶片中表达量。摘花蕾处理能上调叶片中LlAGPS1的表达量，同时该基因在叶片和珠芽中的表达量日变化均呈现下降规律。LlAGPS1基因编码蛋白具有cl33437家族特有的PLN02241、GlgC保守结构域，是编码AGPase蛋白的同源基因，具有明显的组织表达特异性，在珠芽生长发育过程中呈现高表达。本研究结果可为后续研究LlAGPS1基因表达调节技术对卷丹百合珠芽淀粉合成及生长发育的影响，以及开发珠芽价值提供参考。

关键词: 卷丹百合, 珠芽, 腺苷二磷酸葡萄糖焦磷酸化酶, LlAGPS1, 基因表达

Abstract:

Adenosine diphosphate glucose pyrophosphorylase (AGPase) is the rate-limiting enzyme in the starch synthesis pathway of plants. Cloning the homologous gene of AGPase in Lilium lancifolium and analyzing its expression characteristics are helpful to understand the law of starch synthesis during the growth and development of bulbils, and provide a basis for the development and utilization of bulbils as a seed resource or dietary ingredient. In this study, a gene encoding a small subunit of adenosine diphosphate glucose pyrophosphorylase (LlAGPS1) was cloned from Lilium lancifolium by homologous cloning technology. The expression of LlAGPS1 in leaves and bulbils was analyzed by fluorescence quantitative PCR. The results showed that the protein encoded by LlAGPS1 gene had PLN02241, GlgC conserved domain and belonged to cl33437 super family protein. Protein multiple sequence alignment showed that it had high homology with AGPase small subunit sequences of other crops. The qPCR analysis showed that LlAGPS1 gene was mainly expressed in bulbils, which was significantly higher than that in leaves. The flower bud removal treatment could up-regulate the expression of LlAGPS1 in leaves, and the diurnal variation of the gene expression in leaves and bulbils showed a decreasing trend. The protein encoded by LlAGPS1 gene has PLN02241 and GlgC conserved domains unique to cl33437 family. It is a homologous gene encoding AGPase protein with obvious tissue expression specificity and high expression in the growth and development of bulbils. The results provide a reference for the follow-up study of the effect of LlAGPS1 gene expression regulation technology on the starch synthesis, growth and development of Lilium lancifolium bulbils, and the development of bulbil value.

Key words: Lilium lancifolium, bulbil, adenosine diphosphate glucose pyrophosphorylase, LlAGPS1, gene expression