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中国农学通报

中国农学通报 ›› 2025, Vol. 41 ›› Issue (33): 121-130.doi: 10.11924/j.issn.1000-6850.casb2024-0753

• 生物科学 • 上一篇    下一篇

桂花OfBBXs基因家族鉴定及成花调控基因的备选

勾啸1(), 温鹏花1, 阮沐宁1, 方众力2, 张鑫3   

  1. 1 广州市林业和园林科学研究院，广州 510000
    2 广州市白云园林建设工程有限公司，广州 510000
    3 连南瑶族自治县绿连林业发展有限公司，广东清远 513399
  • 收稿日期:2024-12-10 修回日期:2025-02-15 出版日期:2025-11-25 发布日期:2025-12-01
  • 作者简介:

    勾啸，女，1978年出生，林业高级工程师，本科，研究方向：园林植物。通信地址：510080 广州市广园中路428号，广州市林业和园林科学研究院，Tel：13318833316，E-mail：。

  • 基金资助:
    山苍子高效栽培关键技术研究与示范(2025KJQT009)

Identification of OfBBXs Gene Family in Osmanthus fragrans and Screening of Candidate Genes for Flowering Regulation

GOU Xiao1(), WEN Penghua1, RUAN Muning1, FANG Zhongli2, ZHANG Xin3   

  1. 1 Guangzhou Institute of Forestry and Landscape Architecture, Guangzhou 510000
    2 Guangzhou Baiyun Horticulture Construction Engineering Co.,Ltd., Guangzhou 510000
    3 Liannan Yao Autonomous County Lyulian Forestry Development Co., Ltd., Qingyuan, Guangdong 513399
  • Received:2024-12-10 Revised:2025-02-15 Published:2025-11-25 Online:2025-12-01

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摘要：

为筛选参与桂花成花调控的候选基因，本研究以‘日香桂’全基因组为试材，采用生物信息学方法鉴定OfBBX基因家族成员，对其理化性质、系统进化关系、桂花种内及桂花-拟南芥种间共线性关系、组织表达模式进行综合分析，并结合启动子区顺式作用元件预测其功能。结果共鉴定出42个OfBBX基因，其编码蛋白分子量20.54k~53.71 kDa，等电点4.46~8.61，疏水指数-0.904~-0.027。系统进化将OfBBX分为I-V五组（11/7/5/14/7个成员），各组Motif与B-box/CCT结构域高度保守。检测到OfBBX基因发生了串联重复或片段复制事件，与拟南芥存在48对共线BBX基因。其中7个OfBBX基因在花组织中表达量较高，启动子富含光反应型元件；OfBBX19与拟南芥AtBBX1(CONSTANS)同支、共线、同源，其表达量在花器官中显著上调。本研究筛选出OfBBX18/19等7个候选基因，OfBBX19可能是CONSTANS同源基因并介导光周期调控，为桂花花发育机制研究提供分子标记。

关键词: 桂花, BBX, 基因家族, 转录因子, 生物信息学

Abstract:

To screen candidate genes involved in flowering regulation in Osmanthus fragrans, this study used the genome of ‘Rixianggui’ as the experimental material and employed bioinformatics methods to identify members of the OfBBX gene family and analyze the physicochemical properties, phylogenetic evolution, synteny relationships both within Osmanthus fragrans and between Osmanthus fragrans and Arabidopsis, and tissue expression patterns of OfBBX genes, combined with cis-acting element prediction. A total of 42 OfBBX genes were identified, encoding proteins with molecular weights ranging from 20.54k to 53.71 kDa, isoelectric points from 4.46 to 8.61, and grand average of hydropathicity (GRAVY) from -0.904 to -0.027. Phylogenetic analysis analysis classified these genes into five groups (I-V) containing 11, 7, 5, 14, and 7 members, respectively, with highly conserved motif structures and B-box/CCT domains across all groups. Tandem duplication and segmental duplication events were detected in OfBBX genes, and 48 BBX syntenic gene pairs were identified between 'Rixianggui' and Arabidopsis. Seven OfBBX genes showed high expression levels in floral tissues, with their promoters containing abundant light-response elements. Notably, OfBBX19 is phylogenetically clustered with, collinear to, and orthologous with AtBBX1 (CONSTANS) and is significantly up-regulated in floral organs. This study identified seven candidate genes including OfBBX18 and OfBBX19, with OfBBX19 potentially acting as a CONSTANS ortholog mediating photoperiod regulation, thereby providing molecular markers for further investigation into the floral organ development mechanism in Osmanthus fragrans.

Key words: Osmanthus fragrans, BBX, gene family, transcription factor, bioinformatics