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中国农学通报

中国农学通报 ›› 2026, Vol. 42 ›› Issue (9): 145-154.doi: 10.11924/j.issn.1000-6850.casb2025-0050

• 生物科学 • 上一篇    下一篇

花生胚小叶高效离体再生体系的建立与优化

李婷婷1(), 张建成2, 迟晓元2, 姜骁2, 刘晓芹1()   

  1. 1 北京大学现代农业研究院， 潍坊现代农业山东省实验室， 山东潍坊 261000
    2 山东省花生研究所， 山东青岛 266100
  • 收稿日期:2025-01-21 修回日期:2025-05-15 出版日期:2026-05-15 发布日期:2026-05-15
  • 通讯作者:
    刘晓芹，女，1987年出生，山东潍坊人，研究员，博士，研究方向：花生功能基因组学与种质资源创新。通信地址：262113 山东省潍坊市坊子区峡山生态经济开发区滨湖路699号，北京大学现代农业研究院，Tel：0536-6030888，E-mail：。
  • 作者简介:

    李婷婷，女，1997年出生，河南南阳人，科研助理，硕士研究生，研究方向：花生遗传转化。通信地址：262113 山东省潍坊市坊子区峡山生态经济开发区滨湖路699号，北京大学现代农业研究院，Tel：18738375978，E-mail：。

  • 基金资助:
    山东省重点研发计划“基于大数据与基因编辑的野生花生高产抗病性状驯化及种质创制”(2024LZGC035); 泰山学者工程资助“花生分子设计育种与种质资源创新”(tsqn2021031610); 潍坊市科技发展计划项目“花生重要农艺性状调控基因挖掘与育种应用”(2024JZ0016); 财政部和农业农村部：国家现代农业产业技术体系“抗逆品种改良”(CARS-13)

Arachis hypogaea L. Embryo Leaflet: Establishment and Optimization of in Vitro Regeneration System

LI Tingting1(), ZHANG Jiancheng2, CHI Xiaoyuan2, JIANG Xiao2, LIU Xiaoqin1()   

  1. 1 Institute of Advanced Agricultural Sciences of Peking University / Shandong Laboratory of Advanced Agriculture Sciences in Weifang, Weifang, Shandong 261000
    2 Shandong Peanut Research Institute, Qingdao, Shandong 266100
  • Received:2025-01-21 Revised:2025-05-15 Published:2026-05-15 Online:2026-05-15

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摘要：

本研究旨在建立高效的不同品种花生胚小叶离体再生体系，为花生的遗传改良和生物技术应用提供有力的技术支持。本试验以‘莱阳四粒红’、‘83’（‘云花24号’）2个不同品种的花生胚小叶为外植体，采用组织培养法研究不同植物生长调节剂、开盖炼苗时间等对胚小叶再生体系建立的影响。结果表明：‘莱阳四粒红’胚小叶芽丛诱导最适宜的生长调节剂为3.25 mg/L 6-BA+0.2 mg/L NAA，芽丛抽芽为1.5 mg/L TDZ+3.0 mg/L 6-BA+1.0 mg/L GA3，芽丛伸长为2.0 mg/L TDZ+4.0 mg/L 6-BA+0.1 mg/L NAA+1.5 mg/L GA3，生根培养为2.0 mg/L NAA+0.3 mg/L 2,4-D。‘83’（‘云花24号’）胚小叶芽丛诱导最适宜的生长调节剂为3.25 mg/L 6-BA+0.3 mg/L NAA，芽丛抽芽为1.5 mg/L TDZ+4.0 mg/L 6-BA+1.5 mg/L GA3，芽丛伸长为2.0 mg/L TDZ+3.5 mg/L 6-BA+1.0 mg/L NAA+2.0 mg/L GA3，生根培养为2.5 mg/L NAA+0.1 mg/L 2,4-D。2个花生品种炼苗移栽最佳开盖时间为7 d，‘莱阳四粒红’、‘83’（‘云花24号’）再生苗坐果率分别为57.63%、48.67%。本研究成功建立‘莱阳四粒红’和‘83’（‘云花24号’）2个花生品种的胚小叶离体再生体系，明确了各阶段关键调控因子与配比，显著提升再生效率与移栽成活率，成果不仅完善了花生组织培养技术体系，更为后续花生遗传改良研究及功能基因挖掘，提供了重要的转基因技术平台支持。

关键词: 花生, 胚小叶芽丛, 离体再生体系, 组织培养法, 植物生长调节剂

Abstract:

The purpose of this study was to establish an efficient in vitro regeneration system of embryo lobules of different peanut varieties, and to provide strong technical support for genetic improvement and biotechnology application of peanut. In this experiment, the embryo lobules of two different peanut varieties ‘Laiyang Silihong’ and ‘83’ (‘Yunhua 24’) were used as explants, and the effects of different plant growth regulators and seedling hardening time on the establishment of embryo lobule regeneration system were studied by tissue culture method. The results showed that the most suitable growth regulator for ‘Laiyang Silihong’ was 3.25 mg/L 6-BA+0.2 mg/L NAA for bud cluster induction, 1.5 mg/L TDZ+3.0 mg/L 6-BA+1.0 mg/L GA3 for bud cluster extraction, 2.0 mg/L TDZ+4.0 mg/L 6-BA+0.1 mg/L NAA+1.5 mg/L GA3 for bud cluster elongation, and 2.0 mg/L NAA+0.3 mg/L 2,4-D for rooting culture. The most suitable growth regulator for ‘83’ (‘Yunhua 24’) embryo leaflet bud induction was 3.25 mg/L 6-BA+0.3 mg/L NAA, the bud cluster was 1.5 mg/L TDZ+4.0 mg/L 6-BA+1.5 mg/L GA3, the bud cluster elongation was 2.0 mg/L TDZ+3.5 mg/L 6-BA+1.0 mg/L NAA+2.0 mg/L GA3, and the rooting culture was 2.5 mg/L NAA+0.1 mg/L 2,4-D. The best transplanting time of the two peanut varieties was 7 days, and the fruit setting rates of ‘Laiyang Silihong’ and ‘83’ (‘Yunhua 24’) were 57.63% and 48.67%, respectively. In this study, the in vitro regeneration system of embryo leaflets of two peanut varieties, ‘Laiyang Silihong’ and ‘83’ (‘Yunhua 24’), was successfully established. The results not only improved the peanut tissue culture technology system, but also provided important transgenic technology platform support for subsequent peanut genetic improvement research and functional gene mining.

Key words: peanut, embryo leaflet buds, in vitro regeneration system, tissue culture method, plant growth regulators

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