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中国农学通报

中国农学通报 ›› 2026, Vol. 42 ›› Issue (3): 119-124.doi: 10.11924/j.issn.1000-6850.casb2025-0278

• 资源·环境·生态·土壤·气象 • 上一篇    下一篇

中低产土壤接种根际促生细菌对玉米叶片生理特性的影响

刘桂民1(), 宿磊2, 郑晓宁3, 陈迪1(), 刘丙花1, 孙铭婕1, 刘幸红1, 徐英梅4()   

  1. 1 山东省林业科学研究院， 济南 250014
    2 山东省林业科技培训中心， 济南 250013
    3 无棣县检验检测中心， 山东无棣 251900
    4 山东省沂水县杨庄镇农业综合服务中心， 山东临沂 276412
  • 收稿日期:2025-04-26 修回日期:2025-08-15 出版日期:2026-02-15 发布日期:2026-02-09
  • 通讯作者:
    陈迪，男，1977年出生，山东肥城人，本科，研究方向：森林土壤。通信地址：250014 山东省济南市历下区文化东路42号，山东省林业科学研究院，E-mail：；
    徐英梅，女，1980年出生，山东临沂人，副高级工程师，主要从事资源调查规划工作。通信地址：276412 山东沂水县杨庄镇人民政府，沂水县杨庄镇农业综合服务中心，E-mail：。
  • 作者简介:

    刘桂民，男，1974年出生，山东邹平人，研究员，本科，研究方向：植物营养与肥料。通信地址：250014 山东省济南市历下区文化东路42号，山东省林业科学研究院，E-mail：。

  • 基金资助:
    山东省重点研发计划(重大科技创新工程)项目“中低产田改良与产能提升关键技术研究与应用”(2021CXGC010804)

Effects of Inoculating Plant Growth-Promoting Rhizobacteria on Physiological Characteristics of Maize Leaves in Medium- and Low-yield Soils

LIU Guimin1(), SU Lei2, ZHENG Xiaoning3, CHEN Di1(), LIU Binghua1, SUN Mingjie1, LIU Xinghong1, XU Yingmei4()   

  1. 1 Shandong Academy of Forest, Jinan 250014
    2 Shandong Forestry Science and Technology Training Center, Jinan 250013
    3 Inspection and Testing center of Wudi County, Wudi, Shandong 251900
    4 Agricultural Comprehensive Service Center of Yangzhuang Town, Yishui County, Shandong Province, Linyi, Shandong 276412
  • Received:2025-04-26 Revised:2025-08-15 Published:2026-02-15 Online:2026-02-09

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摘要：

针对中低产土壤养分亏缺制约玉米生长的问题，为明确根际促生细菌(PGPR)的缓解效应及机制，以玉米品种‘郑单958’为试材，设置对照、接种培养基、接种灭活菌剂、接种菌剂等4个处理，通过盆栽试验，试剂盒法测定叶片抗氧化酶活性、非酶抗氧化物质、渗透调节物质及内源激素含量等指标，分析PGPR对玉米生理特性的影响。结果表明，与对照相比，接种菌剂处理能显著提高玉米叶片的过氧化物酶(POD)、谷胱甘肽还原酶(GR)活性和非酶抗氧化物质、促进类激素含量，以及IAA/ABA、GA/ABA、ZT/ABA、(IAA+GA+ZT)/ABA比值，显著降低叶片可溶性糖、脯氨酸和脱落酸(ABA)含量，其中POD和GR活性分别较对照显著高出59.46%和56.91%，可溶性糖和ABA含量分别较对照显著降低40.32%和23.87%。此外，与接种菌剂处理相比，接种培养基和灭活菌剂处理对玉米叶片生理特性影响较小。综合分析可知，在玉米根部接种PGPR菌剂，能够通过调节玉米叶片抗氧化系统、渗透调节物质和内源激素含量以及内源激素平衡，进而增强玉米植株在中低产土壤中的适应能力。未来可明确PGPR接种浓度优化方案，开展多点田间试验，结合产量与品质指标，为中低产田玉米提质增效提供更全面的技术支撑。

关键词: 中低产土壤, 根际促生细菌, 玉米, 抗氧化系统, 内源激素平衡, 渗透调节物质, 抗逆性

Abstract:

This study aimed to investigate the effect of inoculating plant growth-promoting rhizobacteria (PGPR) on the physiological characteristics of maize leaves in medium- and low-yield soils. A pot experiment was conducted using the maize variety ‘Zhengdan 958’ as the test material. Four treatments were established, namely the control, inoculation with the culture medium, inoculation with inactivated PGPR, and inoculation with active PGPR. The indices including antioxidant enzymes, non-enzymatic antioxidant substances, osmotic regulatory substances and endogenous hormones in maize leaves were determined by the kit method. The results indicated that compared with the control, the inoculation with PGPR treatment significantly increased the activities of peroxidase (POD) and glutathione reductase (GR), as well as the contents of non-enzymatic antioxidant substances and promoting hormones, and the ratios of IAA/ABA, GA/ABA, ZT/ABA, and (IAA + GA + ZT)/ABA in maize leaves. Conversely, it significantly decreased the contents of soluble sugars, proline, and abscisic acid (ABA) in the leaves, showing 59.46% and 56.91% increases in the activities of POD and GR, as well as 40.32% and 23.87% decreases in the contents of soluble sugars and ABA, in comparison to the control group, respectively. Additionally, compared with the PGPR inoculation treatment, the effects of culture medium inoculation and inactivated PGPR inoculation on the physiological characteristics of maize leaves were relatively smaller. Comprehensive analysis revealed that root inoculation with PGPR could enhance the adaptability of maize plants in medium- and low-yield soils by regulating the antioxidant system, the contents of osmotic adjustment substances and endogenous hormones, and the balance of these hormones in maize leaves.

Key words: medium- and low-yield soils, plant growth-promoting rhizobacteria, maize, antioxidant system, endogenous hormone balance, osmotic adjustment substance, tolerance