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中国农学通报

中国农学通报 ›› 2026, Vol. 42 ›› Issue (9): 138-144.doi: 10.11924/j.issn.1000-6850.casb2025-0605

• 生物科学 • 上一篇    下一篇

甜茶组培快繁技术体系的建立

张亚斐1(), 张成才1,2, 代晓雨2, 张小波1, 王升1,2, 乔萍2, 汪奕衡1,2(), 孙嘉惠1,2()   

  1. 1 中国中医科学院中药资源中心/道地药材品质保障与资源持续利用全国重点实验室， 北京 100700
    2 德兴市中医研究院试验培训基地/中药资源可持续利用江西省重点实验室， 江西德兴 334220
  • 收稿日期:2025-07-18 修回日期:2026-02-25 出版日期:2026-05-15 发布日期:2026-05-15
  • 通讯作者:
    孙嘉惠，女，1989年出生，山东人，副研究员，博士，研究方向：药用植物多样性与系统进化基因组学。通信地址：100700 北京市东城区东直门内南小街16号中国中医科学院中药资源中心，Tel：010-64081790，E-mail：；
    汪奕衡，男，1991年出生，浙江人，助理研究员，博士，研究方向：药用植物群体遗传。通信地址：100700 北京市东城区东直门内南小街16号 中国中医科学院中药资源中心，Tel：010-64081790，E-mail：。
  • 作者简介:

    张亚斐，女，2002年出生，山东人，硕士在读，研究方向：覆盆子群体遗传与品质评价。Tel：010-64081790，E-mail：。

  • 基金资助:
    科技基础资源调查专项“具有潜在药用价值的中药资源调查”(2022FY101004); 中国中医科学院科技创新工程项目资助(CI2026A039); 中国中医科学院科技创新工程项目“道地药材五味子、覆盆子、黄连的药用植物多样性与进化”(CI2023E002); 国家中医药管理局高水平中医药重点学科建设项目“中药资源生态学”(zyyzdxk-2023244); 财政部和农业农村部国家现代农业产业技术体系(CARS-21)

Establishment of Rubus chingii var. suavissimus Tissue Culture Rapid Propagation Technology System

ZHANG Yafei1(), ZHANG Chengcai1,2, DAI Xiaoyu2, ZHANG Xiaobo1, WANG Sheng1,2, QIAO Ping2, WANG Yiheng1,2(), SUN Jiahui1,2()   

  1. 1 State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs/National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700
    2 Dexing Research and Training Center of Chinese Medical Sciences/Jiangxi Key Laboratory for Sustainable Utilization of Chinese Materia Medica Resources, Dexing, Jiangxi 334220
  • Received:2025-07-18 Revised:2026-02-25 Published:2026-05-15 Online:2026-05-15

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摘要：

为解决药用植物甜茶快速繁殖难题，以其带芽嫩茎为外植体，系统研究外植体消毒时间、培养基激素配比及移栽基质配比对无菌系建立、丛生芽增殖、试管苗生根及移栽成活的影响。采用正交试验设计结合逐步回归分析，筛选各环节最优条件。结果表明：甜茶外植体的最佳消毒方式为0.1%升汞溶液消毒7 min，成活率80%；最佳继代增殖培养基为MS+ 6-BA 1.0 mg/L+ NAA 0.1 mg/L，增殖系数为27.33；最佳生根培养基为1/2MS+ IBA 0.5 mg/L，生根率100%，平均生根数约13条，平均根长1.74 cm；最佳移栽土壤基质配比为蛭石:营养土:椰糠=1:1:1，成活率高达86.3%。成功构建甜茶的组织培养快速繁殖体系，该体系繁殖效率高、操作简便，为甜茶优质种质资源扩繁、规模化生产及遗传多样性保护提供了科学依据与技术支撑，未来可进一步优化激素组合与炼苗条件，提升体系稳定性与产业化适配性。

关键词: 甜茶, 覆盆子, 组织培养, 快速繁殖, 离体再生, 生长调节剂, 增殖, 生根

Abstract:

The paper aims to develop a rapid propagation system for the medicinal resource Rubus chingii var. suavissimus. Using tender stem segments with buds as explants, the study investigated the effects of sterilization duration, hormone concentration ratios in subculture proliferation media and rooting media, as well as transplanting substrate ratios on aseptic line establishment, cluster bud proliferation, test-tube plantlet rooting, and transplant growth of tissue-cultured seedlings. Orthogonal test design combined with stepwise regression analysis was used to screen the optimal conditions of each link. Results showed that the optimal sterilization method for explants was treatment with 0.1% HgCl2 solution for 7 minutes, achieving a survival rate of 80%. The optimal subculture proliferation medium was MS+ 6-BA 1.0 mg/L+ NAA 0.1 mg/L, with a proliferation coefficient of 27.33. The optimal rooting medium was 1/2MS+ IBA 0.5 mg/L, resulting in 100% rooting rate, approximately 13 average roots per plant, and average root length of 1.74 cm. The optimal transplanting substrate ratio was vermiculite: nutrient soil: coconut coir=1:1:1, with a survival rate as high as 86.3%. The successful establishment of a rapid tissue culture propagation system for Rubus chingii var. suavissimus holds significant importance for the sustainable development of sweet tea resources and the conservation of genetic diversity in characteristic medicinal plants.

Key words: Rubus chingii var. suavissimus, Rubus chingii, tissue culture, rapid propagation, in vitro regeneration, plant growth regulator, proliferation, rooting

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