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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (18): 202-204.

所属专题： 小麦

• 农学 农业基础科学 • 上一篇    下一篇

PEG胁迫对六倍体小黑麦幼苗SOD POD活性及MDA含量的影响

朱俊刚,王曙光,李晓燕,杨海峰,孙黛珍   

  • 收稿日期:2009-04-03 修回日期:2009-05-19 出版日期:2009-09-20 发布日期:2009-09-20

Effect of PEG stress on the activities of superoxide dismutase (SOD), peroxidase(POD) and the content of Malonaldehyde (MDA)in the seedlings of triticale

  • Received:2009-04-03 Revised:2009-05-19 Online:2009-09-20 Published:2009-09-20

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摘要：

为了解水分胁迫对小黑麦叶片保护酶活性及膜脂过氧化作用的影响，以10个抗旱性不同的六倍体小黑麦为材料，用20%PEG-6000模拟干旱胁迫，研究了不同抗旱性小黑麦品种SOD，POD活性及MDA含量对水分胁迫的反应。结果表明，各品种SOD，POD活性及MDA含量均显著高于对照，但是不同品种增加幅度明显不同。其中, SOD活性胁迫系数依次为S4＞S2＞S7＞S8＞S6＞S9＞S10＞S1＞S5＞S3， 最大是品种S4（Prego）为7.48； POD活性胁迫系数依次为S2＞S9＞S6＞S4＞S8＞S7＞S5＞S10＞S1＞S3，最大是S2（Fidelio）为3.19；而MDA含量胁迫系数依次为S3＞S10＞S1＞S5＞S8＞S2＞S7＞S6＞S9＞S4，最大是S3（Tewo）为4.71, 推断在所有品种中，Prego的抗旱性最强，而Tewo抗旱性则较弱。

关键词: 苹果（Malus domestica B.）, 苹果（Malus domestica B.）, 细胞质型苹果酸酶, 半定量RT-PCR, 原核表达

Abstract:

The effect of water deficit on leaf protective enzymes and permeability of plasma membrane of ten triticale cultivars with different drought resistance was studied.Under 20 % PEG 6000 simulated drought stress , superoxide dismutase (SOD) activity，peroxidase(POD) activity and Malonaldehyde (MDA) content of triticale with different drought resistance were measured .The results showed that the activities of superoxide dismutase (SOD)，peroxidase(POD) and Malonaldehyde (MDA) content are higher than that of control. While they changed apparently between different cultivars. The stress coefficient of SOD activity are as follows: S4＞S2＞S7＞S8＞S6＞S9＞S10＞S1＞S5＞S3, the biggest of which is S4(Prego) that is 7.48, the stress coefficient of POD activity are as follows: S2＞S9＞S6＞S4＞S8＞S7＞S5＞S10＞S1＞S3，the biggest of which is S2(Fidelio) that is 3.19 , and the stress coefficient of MDA content are as follows: S3＞S10＞S1＞S5＞S8＞S2＞S7＞S6＞S9＞S4, the biggest of which is S3(Tewo) that is 4.71 ,This indicted that Prego might was a cultivar with the strongest drought resistance and Tewo might was drought-sensitive cultivar in all cultivars.