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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (4): 43-46.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

小金海棠超氧化物歧化酶基因MxSod2克隆与序列分析

张玉刚,郭绍霞,韩振海   

  • 收稿日期:2008-10-30 修回日期:2008-12-18 出版日期:2009-02-20 发布日期:2009-02-20

Cloning and Sequence Analysis of Superoxide dismutase gene (MxSod2) in Malus xiaojinensis

  • Received:2008-10-30 Revised:2008-12-18 Online:2009-02-20 Published:2009-02-20

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摘要： 本实验以苹果属植物小金海棠（Malus xiaojinensis Cheng et Jiang）为试材，利用差异筛选消减cDNA文库和RACE相结合的方法，分离到了小金海棠超氧化物歧化酶基因MxSod2（Genbank登录号为 DQ431473），该基因全长805个碱基，开放阅读框为471bp，编码157个氨基酸，推测分子量15kDa。该基因具有CuZn-SOD基因的典型结构域特征。通过系统进化树分析，小金海棠MxSod2与龙眼、荔枝等非禾本科植物保持了较近的亲缘关系，与花生、水稻、玉米等禾本科植物亲缘关系较远。

关键词: 石油污染, 石油污染, 土壤修复, 植物筛选

Abstract: A superoxide dismutase gene was cloned by differential screening of the subtracted cDNA library and RACE from Malus xiaojinensis Cheng et Jiang, which was named MxSod2 (Accession No. DQ431473). The full-length cDNA of MxSod2 is 805 bp, including the open reading frame (ORF) of 471 bp. It encodes a protein of 157 amino acids, with a molecular weight of 15 kDa. The MxSod2 contained the conserved domains shared in all known SOD. The phylogenetic tree suggested that MxSod2 was closer to the SOD of non-graminaceous plants including longan and litchi, but farther with the SOD of peanut, rice and maize.