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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (4): 47-49.

所属专题： 玉米

• 生物技术科学 • 上一篇    下一篇

糯玉米的成熟胚培养

何婷,王亦菲,陆瑞菊,等   

  • 收稿日期:2008-12-01 修回日期:2008-12-30 出版日期:2009-02-20 发布日期:2009-02-20

Mature Embryo Culture in Waxy Maize

  • Received:2008-12-01 Revised:2008-12-30 Online:2009-02-20 Published:2009-02-20

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摘要： 以糯玉米成熟胚为外植体作离体培养，研究在培养基中添加植物激素，硝酸银及有机物等对玉米愈伤组织的诱导和胚性愈伤继代生长的影响。结果表明：在诱导培养阶段，N6+6-BA 0.2mg/L+2,4-D 2mg/L的培养基诱导率最高，愈伤质量好；在继代培养阶段，MS+6-BA 0.2mg/L+2,4-D 2mg/L +AgNO310mg/L或CW (Coconut Water) 10%的培养基可改善胚性愈伤的质量。

关键词: 土壤, 土壤, 肥料, 配方施肥, 信息技术

Abstract: Mature waxy maize embryos were cultured as explants in vitro, and the effects on callus induction and subculturing were investigated under culture mediums with different additives, such as plant hormones, AgNO3, organic compounds, etc. The results showed that the combination of N6 + 0.2mg/L 6-BA + 2mg/L 2,4-D could obtain the most calli of higher quality in the induction phase, while the combination of MS + 0.2mg/L 6-BA + 2mg/L 2,4-D +10mg/L AgNO3 or 10% CW could make sure the calli of higher quality further in the stage of subculturing. Key words: Waxy maize, Mature embryo, Embryogenic callus