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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (12): 22-25.

所属专题： 园艺

• 生物技术科学 • 上一篇    下一篇

花椰菜RAPD随机引物的筛选

陈阳1,周先治1,陈晟1,吴宇芬1,赵依杰2   

  • 收稿日期:2010-01-26 修回日期:2010-02-22 出版日期:2010-06-20 发布日期:2010-06-20
  • 基金资助:

    省科技厅重点项目“台湾十字花科、葫芦科蔬菜良种引进筛选及栽培技术研究”;省自然基金“镧对西瓜光合系统能量传递和强光防御机制调节作用的研究”

Screening Random Primers for RAPD analysis in Cauliflower Cultivars

Chen Yang1, Zhou Xianzhi1, Chen Sheng1, Wu Yufen1, Zhao Yijie2   

  • Received:2010-01-26 Revised:2010-02-22 Online:2010-06-20 Published:2010-06-20

PDF (PC)

1089

摘要：

【研究目的】为了解37份花椰菜种质资源的遗传多样性和亲缘关系，为花椰菜育种提供理论基础，本研究以其中7份来自不同产地的花椰菜品种为模板对80条随机引物进行了初步筛选。【结果】48条随机引物能获得多态性扩增，25条引物的RAPD分析结果无多态性，7条引物的未获得扩增条带；【结论】 引物 S11、S15、S16、S17、S18和 S65 扩增条带比较稳定、清晰且具有多态性，多态性比例在 16.7% - 75.0% 之间，可作为花椰菜RAPD分析的随机引物。

关键词: PDA, PDA, 水稻, 精确施氮, 决策支持系统

Abstract:

【OBJECTIVE】To elucidate the genetic diversity and phylogenetic relationships of 37 cauliflower cultivars, provide a theoretical basis for cauliflower breeding, 80 random primers were carried out a preliminary screening with 7 among the cauliflower cultivars as amplification template. 【RESULTS】Among which 48 primers gave polymorphism, 25 primers gave monomorphic and 7 primers without amplified bands within the varieties. 【CONCLUSION】Stable, clear and polymorphic bands could be obtained with primers S11, S15, S16, S17, S18 and S65, the percentage of polymorphic bands ranging from 16.7% to 75.0%, and these primers appear to be useful for genetic diversity studies in cauliflower cultivars.