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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (13): 294-297.

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

甜瓜SSR引物在西葫芦中的通用性研究

刘栓桃 赵智中 董艳敏 卢亚楠 卢金东 何启伟   

  • 收稿日期:2010-01-15 修回日期:2010-03-01 出版日期:2010-07-05 发布日期:2010-07-05
  • 基金资助:

    西葫芦SSR分子标记做图F2代及双单倍体(DH)群体构建与SSR引物的开发;西葫芦SSR引物开发及分子标记连锁图谱构建;大白菜、西葫芦种质创新与分子标记研究及高效育种技术体系构建

Transferability of SSRs from Cucumis melo to Summer squash

  • Received:2010-01-15 Revised:2010-03-01 Online:2010-07-05 Published:2010-07-05

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摘要：

本文分析了25对甜瓜SSR引物在西葫芦基因组中的可转移性，以期为西葫芦分子遗传育种研究提供分析工具。结果表明，约24%的甜瓜SSR引物可在西葫芦基因组中有效扩增。多态性分析发现6对引物中有4对在34份西葫芦种质中可以检测到多态性，等位变异数从3~6不等，平均为4。UPGMA 聚类分析表明，来自市场主栽F1的纯化材料和双单倍体材料之间多样性较差，说明现有栽培品种基因谱较窄；而从国家蔬菜种质资源库引进的材料多样性比较丰富，对这些材料的整理和重新评价有助于发掘新的基因源，为西葫芦杂交育种提供新材料。

关键词: 非直线双曲线模型, 非直线双曲线模型, 净光合速率, 季节变异规律, 半同胞家系, 湿地松

Abstract:

Simple sequence repeat (SSR) is a popular tool in genetic studies. Currently, only a few SSR markers are available in summer squash (Cucurbita pepo L.), whereas plenty of SSR markers have been developed in its close relative Cucumis melo. In this study, 25 SSR primer pairs from Cucumis melo were tested in summer squash. Approximately 24% of them generated repeatable and clear products in summer squash, indicating a relative high homology between genomes of the two species. Of the transferable SSR markers, 6 primer pairs generated polymorphic products in 34 summer squash genotypes with a total of 16 alleles. Based on the SSR fingerprints, a relative low level of polymorphism was detected within the materials originated from F1 hybrid commonly seen in market while a high level of polymorphism was detected in those introduced from National Vegetable Ggermplasms Resource Center（NVGRC）