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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (14): 232-237.

• 林学 园艺 园林 • 上一篇    下一篇

21个兰花品种的延长RAPD(ERAPD)和PCR-RFLP分析

蹇黎 武海 张以忠 于丹凤 秦小军 朱利泉   

  • 收稿日期:2010-03-22 修回日期:2010-04-02 出版日期:2010-07-20 发布日期:2010-07-20
  • 基金资助:
    贵州省教育厅自然科学研究项目

Molecular Characterization of Chinese cymbidium based on Extended RAPD (ERAPD) and PCR-RFLP Markers

  • Received:2010-03-22 Revised:2010-04-02 Online:2010-07-20 Published:2010-07-20

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摘要： 【研究目的】明确中国兰花品种间在DNA水平上的遗传多样性和亲缘关系。【方法】应用ERAPD（延长随机引物扩增）、叶绿体和线粒体基因组PCR-RFLP对21个中国兰花品种进行分子鉴定。【结果】RAPD分析中共有8个(10%)引物和88条(83.81%)多态性带纹；ERAPD分析中能获得一条2.5KbRAPD特异性片段；叶绿体基因组（cpDNA）的PCR-RFLP分析中有4个（66.67%）引物和12个(25%)引物-酶组合；线粒体基因组中（mtDNA）的PCR-RFLP分析中有2个（33.33%）引物和1个（4%）引物-酶组合能揭示材料间遗传差异。【结论】3种分子标记均能揭示品种间在DNA水平上存在遗传差异，而且遗传学分类与形态学分类基本吻合。

关键词: 玉米, 玉米, 马唐, 间作, 重金属, 生物富集

Abstract: 【objective】In order to reveal the genetic variations and relationship of 21 Chinese cymbidium cultivars.【Method】21 cultivars from 7 cymbidium species were analyzed by ERAPD, cpDNA and mtDNA PCR-RFLP markers.【Result】Eighty-eight polymorphic bands are observed in 8 out of 80 random primers (10%) in RAPD analyses, the 2.5-kb RAPD fragment are obtained in ERAPD analysis, Genetic difference are revealed in 4 primers （66.67%）and 12 primer-enzyme combinations(25%) by cpDNA PCR-RFLP analysis, 2 primers （33.33%）and 1 primer-enzyme combinations(4%) by mtDNA PCR-RFLP analysis.【conclusion】The results indicate that these molecular markers can detect the genetic difference in 21 Chinese cymbidium cultivars.