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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (15): 227-232.

所属专题： 玉米

• 农学 农业基础科学 • 上一篇    下一篇

羧甲基壳聚糖-铜(Ⅱ)-多吡啶型SOD模拟物的制备及其对玉米幼苗抗盐害作用的影响

乐学义 王彩霞   

  • 收稿日期:2010-02-02 修回日期:2010-04-01 出版日期:2010-08-05 发布日期:2010-08-05
  • 基金资助:

    生物质新材料的功能化研究

The Synthesis of SOD Mimics Carboxymethyl Chitosan - Cu(Ⅱ)-Polypyridine and Their Effect on the Salt Stress of Maize Seedlings

  • Received:2010-02-02 Revised:2010-04-01 Online:2010-08-05 Published:2010-08-05

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摘要：

【研究目的】试验旨在合成2个新型羧甲基壳聚糖-铜(Ⅱ)-多吡啶型超氧化物歧化酶模拟化合物，并研究这些模拟物对农作物抗逆作用的影响。【方法】用红外光谱，紫外-可见光谱，原子吸收光谱对这些抹模拟物: cmcts-Cu(Ⅱ)-bpy（1）和cmcts-Cu(Ⅱ)-phen（2）(cmcts=羧甲基壳聚糖, bpy=2,2’-联二吡啶，phen=1,10-邻菲咯啉)进行了表征；并研究了模拟物在中度土壤盐害的情况下对玉米幼苗抗盐害作用的影响。【结果】结果表明：在中度盐害情况下，经这些SOD模拟物浸种、叶面喷洒处理，发现生玉米物量提高2.38~53.02 %（茎叶长）和25.00~64.29 %（鲜重），体内SOD活性提高最高达95.99 %。【结论】初步表明这些SOD模拟物具有较高的SOD活性，能有效抑制玉米幼苗所受的盐害胁迫作用。

关键词: DUS测试, DUS测试, 数量性状, 样本容量, 置信区间, 参数估计

Abstract:

【OBJECTIVE】The experiment was conducted to synthesize two novel superoxide dismutase complexes, and study their effect on the resistance environmental stress of crop.【METHOD】The SOD mimics: cmcts-Cu(Ⅱ)-bpy（1）and cmcts-Cu(Ⅱ)-phen（2）(cmcts= carboxymethyl chitosan, bpy=2,2’-bipyridine，phen=1,10-pehnanthroline) were characterized by infrared absorption spectrum,electronic absorption spectrum and atomic absorption spectrometry, and the effect of the SOD Mimics on the salt stress of maize seedlings has been studied by the way of soaking and spraying 0.08~0.24 mg?L-1 SOD mimics.【RESULTS】The results showed that the SOD mimics increased the biomass [2.38~53.02 %(leaf),25.00~64.29 %(fresh)],and enhanced the high SOD activities (0~95.99 %) of the maize seedlings.【CONCLUSION】The SOD mimics had good SOD-like activities, and could effectively protect the maize seedlings against the salt stress.