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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (1): 100-104.

所属专题： 生物技术； 油料作物

• 农学 农业基础科学 • 上一篇    下一篇

抗草甘膦转基因大豆对根际土壤细菌多样性的影响

李刚 赵建宁 杨殿林   

  • 收稿日期:2010-07-02 修回日期:2010-07-28 出版日期:2011-01-07 发布日期:2011-01-07
  • 基金资助:

    转基因生物新品种培育重大专项课题;中央级公益性科研院所基本科研业务费专项资金（农业部环境保护科研监测所）资助项目

Effects of Glyphosate Resistant Transgenic Soybean on Bacterial Diversity in Rhizospheric Soil

  • Received:2010-07-02 Revised:2010-07-28 Online:2011-01-07 Published:2011-01-07

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摘要：

采用DGGE-cloning测序技术研究抗草甘膦转基因大豆在生长期内对根际土壤细菌多样性的影响。结果分析表明，不同生长期内抗草甘膦转基因大豆与非转基因大豆根际土壤细菌16S rDNA DGGE指纹图谱谱形相似，仅在出苗期和鼓粒期出现两条差异带，分别属于芽单胞菌门（Uncultured Gemmatimonadetes bacterium clone，缺失）和壁厚菌门（Uncultured Firmicutes bacterium clone GASP-KC3W1_H04，增加）；不同生长期内抗草甘膦转基因大豆与非转基因大豆根际土壤细菌16S rDNA DGGE指纹图谱的多样性指数和均匀度指数无显著差异。因此，抗草甘膦转基因大豆对土壤细菌多样性无显著影响。

关键词: ACCase, ACCase, accB, 载体构建, 原核表达, IPTG诱导

Abstract:

DGGE-cloning sequencing method was chosen to study the effects of the planting of glyphosate resistant transgenic soybean (Roundup Reandy Soybean, RRS) on bacterial diversity in the rhizospheric soil during the growing period. The results showed that the rhizospheric bacterial 16S rDNA DGGE fingerprintings between glyphosate resistant transgenic soybean and non-transgenic soybean were similar within different growing stages, with only two different bands in the stages of emerging and feed filling, the two bands belonged to uncultured Gemmatimonadetes bacterium clone (vanished) and uncultured Firmicutes bacterium clone GASP-KC3W1_H04 (appeared) respectively; the diversity index and evenness index of 16S rDNA DGGE fingerprintings between glyphosate resistant transgenic soybean and non-transgenic soybean were with no significant differences. The glyphosate resistant transgenic soybean had no significant effects on the soil bacteria.

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