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中国农学通报 ›› 2011, Vol. 27 ›› Issue (27): 272-276.

• 植物保护 农药 • 上一篇    下一篇

内生真菌HND5分子鉴定及其生物学特性研究

刘先宝 郭志凯 时涛 王永壮 霍姗姗 黄贵修   

  • 收稿日期:2010-08-18 修回日期:2010-09-29 出版日期:2011-10-25 发布日期:2011-10-25

Molecular Identification and Biological Characteristics of Endophytic HND5

  • Received:2010-08-18 Revised:2010-09-29 Online:2011-10-25 Published:2011-10-25

摘要:

HND5具有内生性并且对多种病原真菌有拮抗作用,是一株很有应用潜力的生防菌株。本研究对该菌株核糖体内转录间隔区和β微管蛋白基因内转录间隔区进行了分析,其序列和紧密枝顶孢同源性最高。生物学特性研究表明其生长最适培养基为PDA,最适温度为28℃,最适pH为7,最适碳氮源分别为蔗糖和硝酸钠,菌株对重铬酸钾具有耐受性。

关键词: 阳离子盐, 阳离子盐, 奶牛, 生产性能, 离子平衡

Abstract:

HND5 is an endophytic fungi and had antagonistic activity on varieties of pathogenic fungi, so it had application potential for biocontrol. Sequences analysis of ribosomal DNA and β-tubulin internal transcribed spacer region show HND5 had the highest homologous with Acremonium strictum. Biological characteristics research showed the optimum medium was PDA, the optimum temperature was 28℃, and the optimum pH was 7. The optimum carbon and nitrogen source were cane sugar and NaNO3, respectively. HND5 had the tolerance for potassium dichromate.