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中国农学通报

中国农学通报 ›› 2019, Vol. 35 ›› Issue (30): 49-57.doi: 10.11924/j.issn.1000-6850.casb18060076

所属专题： 生物技术； 园艺

• 林学 园艺 园林 • 上一篇    下一篇

牛蒡根酶解物的制备及其对甜瓜生长与品质的影响

寇娟妮1, 辛寒晓2, 孙国科2, 刘丽英1, 孙中涛1   

  1. 1.山东农业大学生命科学学院;2.山东佐田氏生物科技有限公司
  • 收稿日期:2018-06-17 修回日期:2019-09-27 接受日期:2018-12-20 出版日期:2019-10-25 发布日期:2019-10-25
  • 通讯作者: 孙中涛
  • 基金资助:
    山东省科技重大专项“盐渍土快速改良与地力培肥产品的研发与应用”(2017CXGC0306)；济南市科学技术发展计划项目“大棚盐渍化与酸 化土壤改良专用生物肥料的生产技术与应用示范”(201704026)；山东农业大学泉林黄腐酸肥料工程实验室开放研发基金项目“抗病-促生微生物与生 物腐植酸联用研制新型微生物肥料”(QL2016-6)；山东省西部经济隆起带和省扶贫重点区域人才项目“综合利用菌糠制备有机水溶肥和双源腐植酸 生物有机肥的关键技术研究与应用示范”（2017 年）。

Hydrolysate of Burdock Root: Preparation and Its Effect on Melon Growth and Quality

  • Received:2018-06-17 Revised:2019-09-27 Accepted:2018-12-20 Online:2019-10-25 Published:2019-10-25

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摘要： 为了提高牛蒡根下脚料酶解物的收率并验证其肥效，笔者采用响应面法对复合酶的组成和酶解条件进行优化，并通过盆栽试验验证酶解物对甜瓜生长和品质的影响。结果表明，复合酶的最佳组成为纤维素酶47.3%、中性蛋白酶1.0%、碱性蛋白酶51.7%，最佳酶解条件为49.9℃、pH 6.42、底物浓度6.94%、酶底比2.536%。在此条件下水解3 h，牛蒡根酶解物的收率可达74.83%，可溶性蛋白得率可达11.06%，可溶性糖得率可达37.49%。牛蒡根酶解物富含有机质、可溶性糖和氨基酸等肥效成分，具有促进甜瓜生长，提高其产量和品质的作用。复合酶法水解牛蒡根下脚料具有技术可行性，酶解产物收率高且富含有机养分，可用作水溶性有机肥料。

关键词: 水稻, 水稻, 多蘖矮杆, 基因定位, htd7(t)

Abstract: The paper aims to increase the yield of the enzymatic hydrolysate of burdock root waste and to test its fertilizer efficiency. We optimized the complex enzyme composition and the enzymatic hydrolysis conditions by adopting the response surface method, and tested the effects of the enzymatic hydrolysate on the growth and quality of melon through the pot experiments. The results showed that: the optimum composition of the complex enzyme was cellulase 47.3%, neutral protease 1.0%, and alkaline protease 51.7%, the optimum hydrolysis conditions were 49.9℃, pH 6.42, substrate concentration 6.94%, and the ratio of enzyme to substrate 2.536%; when burdock root waste was hydrolyzed under the optimized conditions for 3 h, the yield of the enzymatic hydrolysate was 74.83%, the yield of soluble protein was 11.06%, and the yield of soluble sugar was 37.49%. The enzymatic hydrolysate was rich in organic matter, soluble sugar and amino acids, which could promote the growth of melon and improve its yield and quality. It is technically feasible to hydrolyze burdock root waste with complex enzyme; the enzymatic hydrolysate is of high yield and rich in organic nutrients which can be used as water-soluble organic fertilizer.