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中国农学通报

中国农学通报 ›› 2020, Vol. 36 ›› Issue (12): 104-110.doi: 10.11924/j.issn.1000-6850.casb19010025

所属专题： 生物技术

• 生物科学 • 上一篇    下一篇

苎麻体细胞植株再生体系优化研究

何鹏亮, 蒋美艳, 邓淑琼, 伍旭东, 揭雨成(), 邢虎成   

  1. 湖南农业大学苎麻研究所,长沙 410128
  • 收稿日期:2019-01-05 修回日期:2019-01-31 出版日期:2020-04-25 发布日期:2020-04-21
  • 通讯作者: 揭雨成
  • 作者简介:何鹏亮,男,1995年出生,湖南郴州人,在读硕士。通信地址：410128 湖南省长沙市芙蓉区 湖南农业大学苎麻研究所101室,E-mail： 1525676750@qq.com
  • 基金资助:
    国家自然基金项目“矿区野生苎麻镉富集性和耐性及分子标记与相关基因的克隆”(31371704);“国家饲草与饲纤兼用作物种质资源平台”(NICGR2018-072);“南方饲草与饲纤兼用作物保护与利用”(2018NWB030-24)

Plant Regeneration System Optimization of Ramie Somatic Cells

He Pengliang, Jiang Meiyan, Deng Shuqiong, Wu Xudong, Jie Yucheng(), Xing Hucheng   

  1. Institute of Ramie, Hunan Agricultural University, Changsha 410128
  • Received:2019-01-05 Revised:2019-01-31 Online:2020-04-25 Published:2020-04-21
  • Contact: Yucheng Jie

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摘要：

旨在探索苎麻体细胞植株再生体系的优化方案,为苎麻组织培养及品种改良提供理论与技术依据。本试验以‘湘苎3号’为试材,优化苎麻愈伤诱导植株再生体系,再将此优化的再生体系应用于其他4个种质材料,得出5个种质的愈伤诱导率、芽诱导率、再生苗生根率,来比较不同基因型之间的体细胞植株再生率差异。结果表明,‘湘苎3号’最佳愈伤诱导培养基为附加2,4-D (0.1 mg/L)和6-BA (2.5 mg/L)的MS培养基;‘湘苎3号’、‘咸丰大叶绿’、‘A’、‘B’、‘中苎1号’的愈伤诱导率分别是：83.3%、80.0%、76.7%、70.0%、53.3%。‘湘苎3号’最佳芽诱导培养基为附加TDZ (0.5 mg/L）、2,4-D (0.02 mg/L)和IAA (0.03 mg/L)的1/2MS培养基,‘湘苎3号’、‘咸丰大叶绿’、‘A’、‘B’、‘中苎1号’的芽诱导率分别是：28.4%、21.2%、12.8%、8.9%、4.9%。‘湘苎3号’最佳生根培养基为附加TDZ (0.02 mg/L)+NAA (0.05 mg/L)+IAA (0.02 mg/L)的MS培养基,‘湘苎3号’、‘咸丰大叶绿’、‘A’、‘B’、‘中苎1号’的生根率都是100%。

关键词: 苎麻, 体细胞, 组织培养, 愈伤组织, 植株再生

Abstract:

This experiment aims at exploring the optimization scheme of plant regeneration system of ramie somatic cell and provide theoretical and technical basis for tissue culture and variety improvement of ramie. In this experiment, ‘Xiangzhu 3’ was used as the test material to optimize the plant regeneration system of ramie callus induction, and then the optimized regeneration system was applied to the other four germplasms of materials, and the callus induction rate, the bud induction rate and the regenerated seedling rooting rate of the five germplasms were obtained. Differences of plant regeneration rates of somatic cells were compared among different genotypes. The results showed that the optimum callus induction medium for ‘Xiangzhu 3’ was MS medium supplemented with 2,4-D (0.1 mg/L) and 6-BA (2.5 mg/L); the callus induction rate of ‘Xiangzhu 3’, ‘Xianfeng Dayelv’, ‘A’, ‘B’ and ‘Zhongzhu 1’ was 83.3%, 80.0%, 76.7%, 70.0% and 53.3%, respectively. The optimum bud induction medium for ‘Xiangzhu 3’ was 1/2MS medium supplemented with TDZ (0.5 mg/L), 2,4-D (0.02 mg/L) and IAA (0.03 mg/L); the bud induction rate of ‘Xiangzhu 3’, ‘Xianfeng Dayelv’, ‘A’, ‘B’ and ‘Zhongzhu 1’ was 28.4%, 21.2%, 12.8%, 8.9% and 4.9%, respectively. The optimum rooting medium of ‘Xiangzhu 3’ was MS medium supplemented with TDZ (0.02 mg/L) + NAA (0.05 mg/L) + IAA (0.02 mg/L). The rooting rate of ‘Xiangzhu 3’, ‘Xianfeng Dayelv’, ‘A’, ‘B’ and ‘Zhongzhu 1’ were all 100%.

Key words: ramie, somatic cell, tissue culture, callus, plant regeneration

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