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中国农学通报

中国农学通报 ›› 2021, Vol. 37 ›› Issue (7): 59-67.doi: 10.11924/j.issn.1000-6850.casb2020-0032

所属专题： 资源与环境； 烟草种植与生产

• 资源·环境·生态·土壤·气象 • 上一篇    下一篇

海南新收集烟草种质资源的鉴定与遗传多样性分析

向小华1(), 李媛2, 张兴伟2, 刘国祥2, 杨菁3, 赵文涛3, 闻刚4, 邵雨4, 范静苑5(), 吕洪坤1()   

  1. 1中国烟草总公司海南省公司海口雪茄研究所,海口 571100
    2中国农业科学院烟草研究所,山东青岛 266101
    3上海新型烟草制品研究院有限公司,上海 201315
    4海南省烟草公司儋州公司,海南儋州 571700
    5四川中烟工业有限责任公司长城雪茄烟厂,四川什邡 618400
  • 收稿日期:2020-04-23 修回日期:2020-11-11 出版日期:2021-03-05 发布日期:2021-03-17
  • 通讯作者: 范静苑,吕洪坤
  • 作者简介:向小华,男,1987年出生,中级农艺师,博士,研究方向为雪茄烟育种,E-mail： xiangxiaohuacaas@163.com。
  • 基金资助:
    中国烟草总公司海南省公司科技计划项目“雪茄烟种质资源SNP指纹图谱构建及鉴定评价和信息平台建立的研究”(201846000024055);中国烟草总公司海南省公司项目“口含烟优质原料生产技术研究与应用”([110201901010(XX-10)])

Identification and Genetic Diversity Analysis of Newly Collected Tobacco Germplasm Resources in Hainan Province

Xiang Xiaohua1(), Li Yuan2, Zhang Xingwei2, Liu Guoxiang2, Yang Jing3, Zhao Wentao3, Wen Gang4, Shao Yu4, Fan Jingyuan5(), Lv Hongkun1()   

  1. 1Haikou Cigar Research Institute, Hainan Provincial Branch of China National Tobacco Corporation, Haikou 571100
    2Chinese Academy of Agricultural Sciences, Qingdao Shandong 266101
    3Shanghai New Tobacco Institution, Shanghai 201315
    4Hainan Tobacco Company Danzhou Company, Danzhou Hainan 571700
    5The Greatwall Cigar Factory, China Tobacco Sichuan Industrial Co., Ltd., Shifang Sichuan 618400
  • Received:2020-04-23 Revised:2020-11-11 Online:2021-03-05 Published:2021-03-17
  • Contact: Fan Jingyuan,Lv Hongkun

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摘要：

为将海南收集到的23份烟草种质资源编目和保存,通过田间表型性状调查,并利用SSR引物进行遗传多样性分析,构建指纹图谱。结果表明,供试种质的农艺性状和质量性状差异较大,不同农艺性状变异系数为20.69%~29.41%。聚类分析将收集的烟草种质资源分为3个类群,其中第Ⅱ类群的植株最高、叶片最大,可用于高产育种。从2000对SSR引物中筛选出12对扩增清晰、重复性和多态性好的引物,共检测到46个等位基因,平均每个标记3.83个,观测杂合度(Ho)平均值为0.5301,预期杂合度(He)平均值为0.4700。Shannon’s信息指数(I)为0.8930,Nei’s多样性指数(H)为0.4593,遗传多样性相对丰富。UPGMA聚类分析表明,在相似性系数0.206处,可将供试烟草资源分为2个类群。同时,利用12对引物构建了23份烟草种质的指纹图谱,为晒烟品种鉴定体系的研究提供理论参考。研究结果可为晒烟种质资源的鉴定与利用、雪茄烟育种亲本材料的选择等提供科学依据。

关键词: 晒烟, 种质资源, 表型性状, 简单序列重复, 遗传多样性

Abstract:

To catalogue and preserve 23 tobacco germplasm resources collected from Hainan Province, agronomic characters and genetic diversity of these tobacco germplasm resources were investigated, and the fingerprints were constructed. The results showed that the agronomic traits and qualitative traits of tested germplasms varied greatly. The coefficient of variation ranged from 20.69% to 29.41% in different agronomic traits. These collected tobacco germplasms were divided into 3 groups by clustering analysis. The germplasms from the second group were the tallest with the largest leaf, which could be used for high-yield breeding. In addition, 12 pairs of primers with specific amplification were screened from 2000 pairs of SSR primers, and 46 polymorphic bands were obtained. An average of 3.83 alleles was observed for each primer pair with the average heterozygosity (Ho) of 0.5301, and the average expected heterozygosity (He) of 0.4700. Shannon’s index was 0.8930, Nei’s index was 0.4593. The genetic diversity of the 23 tobacco germplasm resources was relatively abundant. These varieties were clustered into two groups at the genetic similarly coefficient of 0.206 by UPGMA cluster analysis. At the same time, 12 pairs of primers were used to construct the fingerprints of the 23 tobacco germplasm resources, which laid a theoretical foundation for the research on the identification system of sun-cured tobacco varieties. The study can provide reference for identification and utilization of sun-cured tobacco germplasm resources and selection of parent materials for cigar breeding.

Key words: sun-cured tobacco, germplasm resources, phenotypic traits, simple sequence repeat (SSR), genetic diversity

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