丛枝菌根(AM)真菌扩培技术研究进展

doi:10.11924/j.issn.1000-6850.casb2021-0427

中国农学通报 ›› 2022, Vol. 38 ›› Issue (9): 115-122.doi: 10.11924/j.issn.1000-6850.casb2021-0427

所属专题：生物技术；植物保护

丛枝菌根(AM)真菌扩培技术研究进展

李少杰¹^,²^,³(), 肖清山¹^,²^,³, 宋福强¹^,², 王歆¹^,²^,³()

¹黑龙江大学生命科学学院,农业微生物技术教育部工程研究中心,哈尔滨 150500
²黑龙江大学生命科学学院,黑龙江省寒地生态修复与资源利用重点实验室,哈尔滨 150080
³黑龙江大学生命科学学院,黑龙江省普通高校分子生物学重点实验室,哈尔滨 150080

收稿日期:2021-04-22 修回日期:2021-08-13 出版日期:2022-03-25 发布日期:2022-04-02
通讯作者: 王歆
作者简介:李少杰,男,1998年出生,山西晋城人,硕士,研究方向：修复生态学。通信地址：150080 黑龙江省哈尔滨市南岗区学府路74号黑龙江大学生命科学学院,Tel：15234632688,E-mail： 2201575@s.hlju.edu.cn。
基金资助:
国家自然科学基金面上项目“氮沉降条件下阔叶红松林功能真菌群落结构的响应及其对土壤有机碳动态的调控”(31971527);黑龙江省自然科学基金研究团队项目“寒区农业土壤面源污染微生物调控技术的研究”(TD2019C002)

Propagation of Arbuscular Mycorrhizal Fungi: A Review

LI Shaojie¹^,²^,³(), XIAO Qingshan¹^,²^,³, SONG Fuqiang¹^,², WANG Xin¹^,²^,³()

¹Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, School of Life Sciences, Heilongjiang University, Harbin 150500
²Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region, School of Life Sciences, Heilongjiang University, Harbin 150080
³Key Laboratory of Molecular Biology, College of Heilongjiang, Heilongjiang University, Harbin 150080

Received:2021-04-22 Revised:2021-08-13 Online:2022-03-25 Published:2022-04-02
Contact: WANG Xin

摘要/Abstract

摘要：

AM真菌在没有植物共生的情况下还无法像其他种类真菌一样在培养基上纯培养,这一问题极大地限制了对AM真菌的基础研究和应用研究。为了明确不同丛枝菌根(AM)真菌培养的特点,最终实现AM真菌的纯培养目标,本文归纳并比较了AM真菌与完整植物体共生培养的方法,包括土壤盆栽法、营养液膜法、汽雾栽培法、培养基培养法;总结了AM真菌与植物根系双重培养的方式,包括原生根培养和转化根培养。同时提出了相关后续的研究重点,以期为AM真菌培养及共生机制研究提供参考。

关键词: 丛枝菌根真菌, 共生培养, 双重培养, 共生机制

Abstract:

In the absence of plant symbiosis, arbuscular mycorrhiza (AM) fungi cannot be purely cultured on medium like other fungi, which greatly restricts the basic and applied research on AM fungi. This study aims at clarifying the development process and characteristics of different culture methods of arbuscular mycorrhiza (AM) fungi, and achieving the goal of pure culture of AM fungi. The authors reviewed and compared the symbiotic culture methods between AM fungi and the whole plant, including pot culture method, nutrient film technique, aeroponics and medium culture. The authors also summarized the dual culture methods between AM fungi and plant roots, including non-transformed root culture and transformed root culture. At last, the authors put forward some future research focuses in order to provide reference for the research of AM fungi culture and symbiotic mechanism.

Key words: arbuscular mycorrhizal fungi, symbiotic culture, dual culture, symbiotic mechanism

中图分类号:

S154.3

李少杰, 肖清山, 宋福强, 王歆. 丛枝菌根(AM)真菌扩培技术研究进展[J]. 中国农学通报, 2022, 38(9): 115-122.

LI Shaojie, XIAO Qingshan, SONG Fuqiang, WANG Xin. Propagation of Arbuscular Mycorrhizal Fungi: A Review[J]. Chinese Agricultural Science Bulletin, 2022, 38(9): 115-122.

图/表 2

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AM真菌种类	繁殖体	宿主根	培养基	培养方式	参考文献
Glomus intraradicesMUCL 49410	孢子	Medicago truncatula	MSR	单室培养	[12]
Glomus intraradicesDAOM 197198	孢子	Lycopersicon esculentum wild-type 76R and rmc	M	单室培养	[13]
Glomus intraradicesMUCL 43204	孢子	Lunularia cruciata	SRV	单室培养	[14]
Glomus intraradices	孢子	Solanum lycopersicum L.	M	单室培养	[15]
Gigaspora margarita	孢子	Lycopersicon esculentum Mill.	MS	单室培养	[16]
Gigaspora margarita	孢子	Linum usitatissimim L. Tagetes pastula L. Althaea offificinalis L.	M	单室培养	[17]
Glomus proliferumMUCL 41827	孢子	Lunularia cruciata	SRV	单室培养	[14]
Glomus mosseae	孢子	Lycopersicum esculentum Mill. Trifolium pratense L.	White	单室培养	[11]
Glomus mosseae	孢子	Saccharum offificinarum Sorghum vulgare	M	单室培养	[18]
Glomus versiforme	囊泡	Solanum lycipersicum L.	M	单室培养	[19]

AM真菌种类	繁殖体	宿主根	培养基	培养方式	参考文献
Glomus intraradicesMUCL 49410	孢子	Medicago truncatula	MSR	单室培养	[12]
Glomus intraradicesDAOM 197198	孢子	Lycopersicon esculentum wild-type 76R and rmc	M	单室培养	[13]
Glomus intraradicesMUCL 43204	孢子	Lunularia cruciata	SRV	单室培养	[14]
Glomus intraradices	孢子	Solanum lycopersicum L.	M	单室培养	[15]
Gigaspora margarita	孢子	Lycopersicon esculentum Mill.	MS	单室培养	[16]
Gigaspora margarita	孢子	Linum usitatissimim L. Tagetes pastula L. Althaea offificinalis L.	M	单室培养	[17]
Glomus proliferumMUCL 41827	孢子	Lunularia cruciata	SRV	单室培养	[14]
Glomus mosseae	孢子	Lycopersicum esculentum Mill. Trifolium pratense L.	White	单室培养	[11]
Glomus mosseae	孢子	Saccharum offificinarum Sorghum vulgare	M	单室培养	[18]
Glomus versiforme	囊泡	Solanum lycipersicum L.	M	单室培养	[19]

丛枝菌根真菌种类	繁殖体	发根农杆菌种类	宿主根	培养基	培养方式	参考文献
Glomus intraradices CMCCROC7	孢子	Ri1600	Solanum tuberosum var ‘Pukhraj'	M	单室培养	[23]
Glomus intraradices MUCL43194	孢子	K599	Astragalua sinicus L.	MSR	单室培养	[24]
Glomus intraradices	孢子	clone Gp	Daucus carota L.	M	单室培养	[25]
	孢子	UNSW	Daucus carota L.	M	单室培养	[26]
	孢子	A4	Lycopersicon esculentum Micro-Tom	M	单室培养	[27]
	孢子	---	Daucus carota L.	M	单室培养	[15]
	孢子	---	Daucus carota L.	M	双室培养	[28]
	囊泡	---	Daucus carota L.	MSR	单室培养	[29]
	孢子	A4T	Medicago truncatula Gaertn.	M	单室培养	[30]
	囊泡	---	Daucus carota L.	M	双室培养	[31]
Rhizophagus intraradices (GC3)	孢子	A4	Daucus carota L.	M+土壤	双室培养	[32]
Rhizophagus irregularis DAOM 181602	孢子	A4	Daucus carota L.	M	单室培养	[33]
Rhizophagus irregularis C3、DAOM 197198	孢子	A4	Daucus carota L.	M、MS	双室培养	[34]
Rhizophagus intraradices	孢子	LBA9402	Lotus japonicus	MSR	单室培养	[35]
	孢子	---	Ocimum basilicum	M	单室培养	[36]
Gigaspora margarita	孢子	A4、LBA9402、ATCC15834	Lycopersicon esculantum L.var. Pusa Ruby.	MS、M	单室培养	[37]
	孢子	clone Gp	Daucus carota L.	M	单室培养	[25]
	孢子	A4	Daucus carota L.	M	单室培养	[38]
Gigaspora decipiens (JA2 strain)	孢子	A4	Daucus carota L.	M	单室培养	[39]
Gigaspora decipiens	孢子	A4	Daucus carota L.	M	单室培养	[40]
Glomus clarum	孢子	A4	Daucus carota L.	M	单室培养	[40]
	孢子	LBA9402	Daucus carota L. Trifolium repens L.	M	单室培养	[41]
Glomus proliferum	囊泡	---	Daucus carota L.	MSR	单室培养双室培养	[42]
Gigaspora gigantea	孢子	A4、R1000	Solanum lycopersicum L.	MSR	单室培养	[43]
	孢子	A4	Daucus carota L.	M	单室培养	[38]
	孢子	----	Daucus carota L.	G+T	单室培养	[44]
Funneliformis mosseae	孢子	K599	Daucus carota L.	MSR	单室培养	[45]
Gigaspora roseae	孢子	A4	Daucus carota L.	M	单室培养	[46]
Scutellospora reticulata	孢子	----	Daucus carota L.	MSR	单室培养	[47]
Acaulospora rehmii	孢子	---	Daucus carota L.	MSR	单室培养	[48]
Glomus caledonium	孢子	----	Daucus carota L.	M	单室培养	[49]
Glomus etunicatum	孢子	A4	Daucus carota L.	M	单室培养	[50]
Glomus fasciculatum	囊泡	---	Daucus carota L.	MSR	单室培养	[29]
Glomus fistulosum	孢子	A4	Fragaria x Ananassa Duchesne.	M	单室培养	[51]
Glomus mosseae	孢子	---	Daucus carota L. Convolvulus sepium L. Solanum tuberosum L. Vigna unguiculata Walp.		双室培养	[21]
	孢子+囊泡	---	Daucus carota L.	M	单室培养	[17]
	孢子	---	Daucus carota L.	M	单室培养	[52]
Glomus macrocarpum	囊泡	---	Daucus carota L.	MSR	单室培养	[29]
Glomus versiforme	囊泡	---	Daucus carota L.	MSR	单室培养	[29]
Rhizophagus aggregatus (GX4)	孢子	A4	Daucus carota L.	M+土壤	双室培养	[32]

丛枝菌根真菌种类	繁殖体	发根农杆菌种类	宿主根	培养基	培养方式	参考文献
Glomus intraradices CMCCROC7	孢子	Ri1600	Solanum tuberosum var ‘Pukhraj'	M	单室培养	[23]
Glomus intraradices MUCL43194	孢子	K599	Astragalua sinicus L.	MSR	单室培养	[24]
Glomus intraradices	孢子	clone Gp	Daucus carota L.	M	单室培养	[25]
	孢子	UNSW	Daucus carota L.	M	单室培养	[26]
	孢子	A4	Lycopersicon esculentum Micro-Tom	M	单室培养	[27]
	孢子	---	Daucus carota L.	M	单室培养	[15]
	孢子	---	Daucus carota L.	M	双室培养	[28]
	囊泡	---	Daucus carota L.	MSR	单室培养	[29]
	孢子	A4T	Medicago truncatula Gaertn.	M	单室培养	[30]
	囊泡	---	Daucus carota L.	M	双室培养	[31]
Rhizophagus intraradices (GC3)	孢子	A4	Daucus carota L.	M+土壤	双室培养	[32]
Rhizophagus irregularis DAOM 181602	孢子	A4	Daucus carota L.	M	单室培养	[33]
Rhizophagus irregularis C3、DAOM 197198	孢子	A4	Daucus carota L.	M、MS	双室培养	[34]
Rhizophagus intraradices	孢子	LBA9402	Lotus japonicus	MSR	单室培养	[35]
	孢子	---	Ocimum basilicum	M	单室培养	[36]
Gigaspora margarita	孢子	A4、LBA9402、ATCC15834	Lycopersicon esculantum L.var. Pusa Ruby.	MS、M	单室培养	[37]
	孢子	clone Gp	Daucus carota L.	M	单室培养	[25]
	孢子	A4	Daucus carota L.	M	单室培养	[38]
Gigaspora decipiens (JA2 strain)	孢子	A4	Daucus carota L.	M	单室培养	[39]
Gigaspora decipiens	孢子	A4	Daucus carota L.	M	单室培养	[40]
Glomus clarum	孢子	A4	Daucus carota L.	M	单室培养	[40]
	孢子	LBA9402	Daucus carota L. Trifolium repens L.	M	单室培养	[41]
Glomus proliferum	囊泡	---	Daucus carota L.	MSR	单室培养双室培养	[42]
Gigaspora gigantea	孢子	A4、R1000	Solanum lycopersicum L.	MSR	单室培养	[43]
	孢子	A4	Daucus carota L.	M	单室培养	[38]
	孢子	----	Daucus carota L.	G+T	单室培养	[44]
Funneliformis mosseae	孢子	K599	Daucus carota L.	MSR	单室培养	[45]
Gigaspora roseae	孢子	A4	Daucus carota L.	M	单室培养	[46]
Scutellospora reticulata	孢子	----	Daucus carota L.	MSR	单室培养	[47]
Acaulospora rehmii	孢子	---	Daucus carota L.	MSR	单室培养	[48]
Glomus caledonium	孢子	----	Daucus carota L.	M	单室培养	[49]
Glomus etunicatum	孢子	A4	Daucus carota L.	M	单室培养	[50]
Glomus fasciculatum	囊泡	---	Daucus carota L.	MSR	单室培养	[29]
Glomus fistulosum	孢子	A4	Fragaria x Ananassa Duchesne.	M	单室培养	[51]
Glomus mosseae	孢子	---	Daucus carota L. Convolvulus sepium L. Solanum tuberosum L. Vigna unguiculata Walp.		双室培养	[21]
	孢子+囊泡	---	Daucus carota L.	M	单室培养	[17]
	孢子	---	Daucus carota L.	M	单室培养	[52]
Glomus macrocarpum	囊泡	---	Daucus carota L.	MSR	单室培养	[29]
Glomus versiforme	囊泡	---	Daucus carota L.	MSR	单室培养	[29]
Rhizophagus aggregatus (GX4)	孢子	A4	Daucus carota L.	M+土壤	双室培养	[32]

丛枝菌根(AM)真菌扩培技术研究进展

Propagation of Arbuscular Mycorrhizal Fungi: A Review

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