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中国农学通报

中国农学通报 ›› 2023, Vol. 39 ›› Issue (2): 8-15.doi: 10.11924/j.issn.1000-6850.casb2021-0657

所属专题： 水稻

• 生物科学 • 上一篇    下一篇

转EPSPS基因抗除草剂水稻恢复系的培育及育种评价

肖本泽(), 王资霖   

  1. 华中农业大学植物科学技术学院，武汉 430070
  • 收稿日期:2021-07-13 修回日期:2021-10-13 出版日期:2023-01-15 发布日期:2023-01-17
  • 作者简介:

    肖本泽，男，1976年出生，湖北江陵人，副教授，博士，研究方向为水稻遗传育种。通信地址：430070 湖北省武汉市洪山区狮子山街1号 华中农业大学植物科技学院，Tel：027-87282130，E-mail：。

  • 基金资助:
    国家转基因专项“抗逆转基因水稻新品种培育”(2016ZX08001003)

Cultivation and Breeding Assessment of Rice Transgenic Restorers Carrying Herbicide-resistant EPSPS Gene

XIAO Benze(), WANG Zilin   

  1. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070
  • Received:2021-07-13 Revised:2021-10-13 Online:2023-01-15 Published:2023-01-17

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摘要：

为了培育既抗除草剂、综合性状又优良的杂交水稻组合，将3份抗除草剂衍生恢复系（华84EP-1、2、3）与5份不育系（红香2A、Y58S、广占63-4S、华1015S、天源6S）配组，对亲本、杂交组合的除草剂抗性和10个主要农艺性状（单株产量、结实率、千粒重、生育期、株高、有效穗数、着力密度、穗总粒数、穗实粒数、穗长）的配合力效应进行了分析。结果表明，所选育的恢复系的除草剂抗性接近完全(>96%)，利用苗期喷施农达可有效地提高杂交品种纯度；恢复系、不育系的一般配合力分别在5个性状（结实率、生育期等）、7个性状（结实率、千粒重等）上达到了显著差异；杂交组合的特殊配合力仅在生育期上达到了显著差异。杂交组合中比对照增产最高的分别是天源6S/华84EP-1、红香2A/华84EP-3和华1015S/华84EP-1。因此，利用抗除草剂恢复系配制水稻杂交组合不仅可以提高品种纯度，也可以培育出强优势组合。

关键词: 抗除草剂, 水稻, EPSPS, 配合力, 杂种优势

Abstract:

For cultivating the herbicide-resistant rice cultivars with excellent agronomic traits, 3 derived herbicide-resistant restorer lines (Hua 84EP-1, 2 and 3) harboring EPSPS gene were mated with 5 sterile lines (Hongxiang2A, Y58S, Guangzhan63-4S, Hua1015S and Tianyuan6S). The herbicide resistance of parents and mated hybrids and the combining ability of 10 main agronomic traits of those mated hybrids, including YP (yield per plant), SSR (seed setting ratio), TGW (1000-grain weight), GP (growth period), PH (plant height), EP (effective panicles per plant), GD (grain density), NG (number of grains per panicle), NFG (number of filled grains per panicle) and PL (panicle length), were analyzed. The results showed that the herbicide resistance of 3 restorer lines was nearly complete (>96%), and it was very effective for improving the purity of rice hybrids to spray herbicide Roundup at seedling stage; the general combining ability of restorer lines and sterile lines reached significant differences in 5 traits (SSR, GP, etc.) and 7 traits (SSR, TGW, etc.) respectively; and the special combining ability of the mated hybrids only reached significant difference in GP. Among these hybrids, Tianyuan6S/Hua84EP-1, Hongxiang2A/Hua84EP-3 and Hua1015S/Hua84EP-1 had the highest yield increase compared with the control. Therefore, using herbicide-resistant restorer lines as male parent can not only improve the purity of hybrids, but also cultivate strong-heterosis combinations.

Key words: herbicide-resistance, rice, EPSPS, combining ability, heterosis