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中国农学通报

中国农学通报 ›› 2023, Vol. 39 ›› Issue (21): 109-117.doi: 10.11924/j.issn.1000-6850.casb2022-0674

• 生物科学 • 上一篇    下一篇

木蓝愈伤组织诱导与不定芽分化培养

贾薇1(), 雍嘉仪1, 李傲天1, 李成1, 马聪慧1, 胡海英1,2()   

  1. 1 宁夏大学农学院，银川 750021
    2 宁夏草牧业工程技术研究中心，宁夏大学，银川 750021
  • 收稿日期:2022-08-28 修回日期:2022-11-27 出版日期:2023-07-25 发布日期:2023-07-24
  • 通讯作者: 胡海英，女，1976年出生，宁夏平罗人，教授，博士，主要从事牧草生理生态、植物生物技术。通信地址：750021 宁夏银川市贺兰山路489号 宁夏大学农学院，Tel：0951-2061351，E-mail：haiying@nxu.edu.cn。
  • 作者简介:

    贾薇，女，1998年出生，宁夏石嘴山人，本科，主要从事牧草生物技术研究。通信地址：750021 宁夏银川市贺兰山路489号 宁夏大学农学院，Tel：18295325625，E-mail：。

  • 基金资助:
    宁夏高等学校一流学科建设（草学学科）资助项目(NXYLXK2017A01); 宁夏大学2021年度大学生创新创业计划项目资助“本氏木蓝离体快繁与移栽技术研究”(2021107490608)

Indigofera bungeana walp.: Callus Induction and Adventitious Bud Differentiation Culture

JIA Wei1(), YONG Jiayi1, LI Aotian1, LI Cheng1, MA Conghui1, HU Haiying1,2()   

  1. 1 School of Agriculture, Ningxia University, Yinchuan 750021
    2 Ningxia Grassland and Animal Husbandry Engineering Technology Research Centre, Ningxia University, Yinchuan 750021
  • Received:2022-08-28 Revised:2022-11-27 Online:2023-07-25 Published:2023-07-24

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摘要：

初步建立了木蓝(Indigofera bungeana Walp.)的离体再生培养体系，为其基因工程育种研究提供前期技术参考。通过种子无菌播种技术获得野生木蓝无菌实生苗，以胚轴、茎段和叶片为外植体，筛选木蓝愈伤组织诱导、不定芽分化、增殖、壮苗培养的最适培养基配方。结果显示，木蓝种子无菌播种发芽率达98%，最适培养基为1/2MS。3种外植体在不同培养基下均能诱导出愈伤组织，根据愈伤组织生长及质地色泽筛选出最适诱导培养基，其中，叶片为MS+0.5 mg/L 6-BA+0.5 mg/L 2,4-D+0.5 mg/L NAA；茎段为MS+0.5 mg/L 6-BA+0.1 mg/L 2,4-D+0.1 mg/L NAA；胚轴为MS+0.1 mg/L 6-BA+0.1 mg/L 2,4-D+0.5 mg/L NAA。相比较叶片和茎段，胚轴来源的愈伤组织适于不定芽分化，最适分化培养基为MS+0.5 mg/L 6-BA+0.1 mg/L 2,4-D+0.1 mg/L NAA和MS+2.0 mg/L 6-BA。不定芽增殖培养基为MS+1.0 mg/L 6-BA+0.1 mg/L NAA，壮苗和生根的最适培养基均为1/2MS。

关键词: 木蓝, 无菌播种, 愈伤组织诱导, 不定芽分化, 生根培养

Abstract:

The system of in vitro regeneration of Indigofera bungeana walp. was established in order to provide a preliminary experimental basis for genetic-engineering breeding of Indigofera bungeana walp.. Aseptic seedlings of Indigofera bungeana walp. were obtained through aseptic seeding. Using plumular axis, stem segments and leaves as explants, the optimum medium formula was selected for callus induction, adventitious bud differentiation, propagation and elongation. The results showed that the germination rate of the Indigofera bungeana walp. seeds reached 98% on the 1/2MS medium. The three explants could be induced callus in different media, and the suitable medium was selected according to the growth, texture and color of callus. For the leaves, the optimum medium was MS+0.5 mg/L 6-BA+0.5 mg/L 2,4-D+0.5 mg/L NAA, for the stem segment, the optimum medium was MS+0.5 mg/L 6-BA+0.1 mg/L 2,4-D+0.1mg/L NAA, for the plumular axis, the optimum medium was MS+0.1 mg/L 6-BA+0.1 mg/L 2,4-D+0.5 mg/L NAA. Compare with stem segments and leaves, the callus from plumular axis were suitable for adventitious bud differentiation, and the optimum medium were MS+0.5 mg/L 6-BA+0.1 mg/L 2,4-D+0.1 mg/L NAA and MS+2.0 mg/L 6-BA. The adventitious bud propagation medium was MS+1.0 mg/L 6-BA+0.1 mg/L NAA, and the optimum medium for elongation and rooting was 1/2MS.

Key words: Indigofera bungeana walp., aseptic seeding, callus induction, adventitious bud differentiation, rooting culture