水稻OsSIG5基因对植物激素和非生物胁迫的响应分析

doi:10.11924/j.issn.1000-6850.casb2024-0420

中国农学通报 ›› 2025, Vol. 41 ›› Issue (14): 32-40.doi: 10.11924/j.issn.1000-6850.casb2024-0420

水稻OsSIG5基因对植物激素和非生物胁迫的响应分析

詹嘉涛¹(), 缪乐怡¹, 纪子贤¹, 王一婷¹, 卢月¹, 谭恩¹, 马帅鹏², 王丽敏¹()

¹ 仲恺农业工程学院农业与生物学院，广州 510225
² 中国热带农业科学院广州实验站，广州 510145

收稿日期:2024-06-27 修回日期:2024-10-15 出版日期:2025-05-15 发布日期:2025-05-14
通讯作者:
王丽敏，女，1984年出生，河北承德人，特聘副教授，博士，主要从事水稻高抗性状的分子机理及高光效育种研究。通信地址：510225广东省广州市海珠区仲恺路501号科学楼四楼，E-mail：wanglimin@zhku.edu.cn。
作者简介:
詹嘉涛，男，2002年出生，广东揭阳人，本科在读，研究方向：水稻叶绿体发育相关基因的克隆及分子机理。通信地址：510225 广东省广州市海珠区仲恺路501号科学楼四楼，E-mail：2906530588@qq.com。
基金资助:
广东省自然科学基金面上项目“OsDG1调控水稻苗期叶绿体发育和冷胁迫响应的分子机制”(2024A1515012960); 仲华基因产业学院项目“水稻AT-hook基因家族成员OsAHLa的功能研究”(KA2103139); 海南省自然科学基金面上项目“水稻粒形基因GL12的图位克隆及设计育种应用”(320MS079)

Response Analysis of OsSIG5 Gene to Phytohormones and Abiotic Stresses in Rice

ZHAN Jiatao¹(), MIAO Leyi¹, JI Zixian¹, WANG Yiting¹, LU Yue¹, TAN En¹, MA Shuaipeng², WANG Limin¹()

¹ College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225
² Guangzhou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Guangzhou 510145

Received:2024-06-27 Revised:2024-10-15 Published:2025-05-15 Online:2025-05-14

摘要/Abstract

摘要：

本研究聚焦水稻(Oryza sativa)sigma因子编码基因OsSIG5，旨在深入探究其对逆境胁迫和激素的响应模式，以期为进一步挖掘OsSIG5及其同源基因的功能提供参考依据。首先，运用生物信息学方法对OsSIG5展开详细分析，结果显示，OsSIG5编码区全长1503 bp，包含6个外显子，编码500个氨基酸。OsSIG5蛋白具有3个σ⁷⁰家族的保守结构域，单子叶植物的SIG5同源蛋白与OsSIG5具有较近的亲缘关系。OsSIG5的启动子区域存在16个植物激素响应元件、20个环境胁迫响应元件。随后，利用实时荧光定量PCR (qRT-PCR)技术，系统分析了OsSIG5在正常条件及胁迫条件下的表达模式。研究发现，OsSIG5主要在水稻叶片中表达。在激素处理方面，经过细胞分裂素(6-BA)处理后，OsSIG5的表达水平显著提高；而在赤霉素(GA₃)、生长素(IAA)、脱落酸(ABA)处理下，其表达水平显著降低。在逆境胁迫处理方面，经过高温、低温、干旱和盐胁迫等逆境条件处理后，OsSIG5的表达水平均显著降低。综上所述，本研究结果清晰表明OsSIG5能够对多种激素和非生物胁迫信号做出响应。

关键词: 水稻, sigma因子, OsSIG5, 系统进化分析, 顺式作用元件分析, 表达模式, 植物激素, 非生物胁迫

Abstract:

Analysis of the response pattern of sigma factor coding gene OsSIG5 in rice (Oryza sativa) to stress and hormones could provide references for further exploring the functions of OsSIG5 and its homologous genes. Firstly, the bioinformatics of OsSIG5 was performed, and the expression patterns of OsSIG5 in rice under normal and stress conditions were analyzed by quantitative PCR (qRT-PCR). The results showed that the coding region of OsSIG5 was 1503 bp in length, containing 6 exons and encoding 500 amino acids. The OsSIG5 protein had three conserved domains of the σ⁷⁰ family, and phylogenetic analyses showed that the SIG5 homologous protein of monocotyledonous plants was closely related to OsSIG5. The promoter region of OsSIG5 contained 16 plant hormone response cis-elements and 20 environmental stress response cis-elements. OsSIG5 was mainly expressed in rice leaves. The expression of OsSIG5 in leaves was significantly increased under 6-Benzylaminopurine (6-BA) treatment, but significantly decreased under gibberellic acid (GA₃), indole acetic acid (IAA) and abscisic acid (ABA) treatment. In addition, the expression level of OsSIG5 in leaves was significantly decreased after high temperature, low temperature, drought and salt stress treatment. This study showed that OsSIG5 could respond to various hormone signals and abiotic stress signals.

Key words: rice, sigma factors, OsSIG5, phylogenetic analysis, cis-acting element analysis, expression pattern, plant hormones, abiotic stress

詹嘉涛, 缪乐怡, 纪子贤, 王一婷, 卢月, 谭恩, 马帅鹏, 王丽敏. 水稻OsSIG5基因对植物激素和非生物胁迫的响应分析[J]. 中国农学通报, 2025, 41(14): 32-40.

ZHAN Jiatao, MIAO Leyi, JI Zixian, WANG Yiting, LU Yue, TAN En, MA Shuaipeng, WANG Limin. Response Analysis of OsSIG5 Gene to Phytohormones and Abiotic Stresses in Rice[J]. Chinese Agricultural Science Bulletin, 2025, 41(14): 32-40.

图/表 8

引物名称	引物序列(5’-3’)
OsSIG5-qF	TCGACTGGAAGCGGATGAAG
OsSIG5-qR	CGCCTCATCTGAAGAACGGA
Actin1-qF	GCATCTCTCAGCACATTCCA
Actin1-qR	ACCACAGGTAGCAATAGGTA

表1. 引物序列信息

图1. OsSIG5基因的结构及蛋白结构域深灰色填充框表示3’UTR区及5’UTR区，黑色填充框表示外显子，黑色细线表示内含子，数字表示外显子碱基数

图2. OsSIG5及其同源蛋白的氨基酸序列比对 XP_015637716.1：水稻(Oryza sativa)；XP_004961057.1：小米(Setaria italica)；XP_025806622.1：黍(Panicum hallii)；XP_039799656.1：柳枝稷(Panicum virgatum)；OEL12738.1：寡桑托穗二花雀稗(Dichanthelium oligosanthes)；XP_047044900.1：硬直黑麦草(Lolium rigidum)；XP_051210581.1：黑麦草(Lolium perenne)；XP_002440351.1：高粱(Sorghum bicolor)；XP_037473750.1：野生二粒小麦(Triticum dicoccoides)；XP_020176520.1：节节麦(Aegilops tauschii subsp. strangulata)；XP_044424429.1：小麦(Triticum aestivum)；XP_008650095.1：玉米(Zea mays)；NP_001330408.1：拟南芥(Arabidopsis thaliana)

图3. OsSIG5及其同源蛋白的进化关系分析

顺式作用元件	数量
光响应	18
生长素响应	3
低温响应	1
水杨酸响应	1
脱落酸响应	10
厌氧诱导相关	1
茉莉酸甲酯反应响应	2
根特异性相关	1
MYB结合位点相关	2

表2. OsSIG5启动子顺式作用元件分析

图4. OsSIG5的表达模式分析 Actin1为内参基因；数据表示为平均值±SD，n=3；**，与根部差异显著(P<0.01)，t检验

图5. OsSIG5对不同激素处理的表达响应 Actin1为内参基因，数据表示为平均值± SD，n=3，**，P < 0.01，t 检验

图6. 非生物胁迫处理后OsSIG5在水稻叶片中的表达水平分析 Actin1为内参基因，数据表示为平均值± SD，n = 3，**，P < 0.01，t检验

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水稻OsSIG5基因对植物激素和非生物胁迫的响应分析

Response Analysis of OsSIG5 Gene to Phytohormones and Abiotic Stresses in Rice

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