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中国农学通报 ›› 2011, Vol. 27 ›› Issue (10): 122-126.

所属专题: 园艺

• 林学 园艺 园林 • 上一篇    下一篇

铁皮石斛不同外植体组培快繁技术比较研究

戴小英 张淑霞 周莉荫 江香梅   

  • 收稿日期:2010-11-15 修回日期:2010-12-31 出版日期:2011-05-15 发布日期:2011-05-15

The Study of Comparative Techniques for Tissue Culture with Different Explants in Dendrobium officinale

  • Received:2010-11-15 Revised:2010-12-31 Online:2011-05-15 Published:2011-05-15

摘要:

运用铁皮石斛不同的外植体进行组培繁殖研究,选择出适宜当前中草药发展合理的繁殖途经。本研究以铁皮石斛种子及茎段为外植体进行组培快繁技术试验,结果表明,二者在诱导初始条件、增殖方式、增殖系数、继代周期、根的诱导分化及苗木移栽等方面存在差异。通过比较试验认为,铁皮石斛快繁体系建立以茎段外植体为宜,建立的繁育体系具有经济高效稳定等优点,为规模化育苗提供物质和技术参考。

关键词: 小麦, 小麦

Abstract:

In this paper, different explants were used in the study on tissue culture of Dendrobium officinale, for the purpose of choosing proper propagation ways of development in medicinal herbs. It’s cultured with seed embryo and stem segments as explants in Dendrobium officinale. The results indicated that there were differences between their tissue cultural techniques, such as initial conditions, propagation procedure, propagation coefficient, subculture cycle, rooting differentiation and seedlings transplanting. According to the comparative test, it’s thought that it was more suitable for constructing the tissue cultural system with the stem segments as explants, which is more economical, more efficient and steady. It’ll be the reference of material and technical bases in large scale seedlings production.