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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (11): 231-235.

所属专题： 农业生态

• 资源 环境 生态 土壤 气象 • 上一篇    下一篇

土壤微生物多样性的研究方法

吴才武 赵兰坡   

  • 收稿日期:2010-11-23 修回日期:2010-12-06 出版日期:2011-05-15 发布日期:2011-05-15
  • 基金资助:

    东北玉米主产区土壤生态环境变化及其监测控制研究

Technologies on Soil Microbiology Diversity

  • Received:2010-11-23 Revised:2010-12-06 Online:2011-05-15 Published:2011-05-15

PDF (PC)

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摘要：

为了更加全面有效地研究土壤微生物多样性，笔者通过对于当前国内外研究土壤微生物多样性的研究方法，包括微生物稀释平板记数法、Biolog微平板法、PLFA分析法、分子生物学方法进行了阐述，说明了几种方法操作性的优缺点，重点说明了在实验过程中应该选取的方法，为研究者选取研究方法时提供基本信息及建议。

关键词: 营养成分, 营养成分

Abstract:

In order to be more effective and completive to research soil microbiology diversity, a series of laboratory methods for the current study soil microbial diversity at home and abroad were reviewed, including plate count, Biolog micro-plates, PFLA analysis and molecular biological techniques. The author illustrated the advantages and disadvantages of several methods, especially emphasis on which method should be selected during the experiment, provided a lot of basic information and advice for researchers.